Accumulation of advanced glycation end products in women with preeclampsia: Possible involvement of placental oxidative and nitrative stress

C. Chekir, Mikiya Nakatsuka, S. Noguchi, H. Konishi, Y. Kamada, A. Sasaki, L. Hao, Y. Hiramatsu

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Advanced glycation end products (AGEs) are known to cause oxidative damage in various cells by binding with its receptor, RAGE. We measured the serum level of AGEs and examined the AGEs, RAGE, and the other biomarkers of oxidative stress in the placentas from preeclamptic women. Competitive ELISA was carried out to measure the AGEs in serum. Western blotting was performed to analyze AGEs and RAGE in the placenta. Immunohistochemical analyses were performed to examine the localization of AGEs, RAGE, and other biomarkers of oxidative stress in the placenta. The mean level of serum AGEs in preeclamptic women was significantly higher than that in healthy non-pregnant women or healthy pregnant women. Western blotting revealed that the level of AGEs or RAGE in preeclamptic placenta was significantly higher than that in normal placenta. Immunohistochemical analyses showed that levels of nitrotyrosine and nitroguanosine, which are formed by reactive nitrogen species, in preeclamptic placenta were higher than those in normal placenta. Accumulation of 4-hydroxy-2-nonenal and 8-hydroxy-2′-deoxyguanosine indicated enhanced oxidative modifications of lipids and DNA in preeclamptic placenta. The AGE-RAGE system, which is upregulated in preeclampsia, is likely to be involved in the oxidative stress of preeclampsia.

Original languageEnglish
Pages (from-to)225-233
Number of pages9
JournalPlacenta
Volume27
Issue number2-3
DOIs
Publication statusPublished - Feb 1 2006

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Keywords

  • 4-Hydroxy-2-nonenal
  • 8-Hydroxy-2′-deoxyguanosine
  • Advanced glycation end product
  • Nitroguanosine
  • Nitrotyrosine
  • Placenta
  • Preeclampsia

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology
  • Developmental Biology

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