Accumulation of aberrant CpG hypermethylation by Helicobacter pylori infection promotes development and progression of gastric MALT lymphoma

Takami Kondo, Takashi Oka, Hiaki Sato, Yoko Shinnou, Kana Washio, Masayuki Takano, Toshiaki Morito, Katsuyoshi Takata, Nobuya Ohara, Mamoru Oouchida, Kenji Shimizu, Tadashi Yoshino

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Aberrant DNA hypermethylation is an important mechanism for the inactivation of tumor-related genes in human tumors. Gastric mucosa-associated lymphoid tissue (MALT) lymphomas arise from Helicobacter pylori-associated chronic gastritis; most patients are H. pylori-positive and eradication therapy is highly effective. In the present study, we used methylation-specific PCR to analyze the DNA methylation status of 11 tumor-related genes (Kip2, p16, hMLH-1, p15, p73, MGMT, DAPK, MINT1, MINT2, MINT31 and HCAD) in 21 specimens of MALT lymphoma, 5 specimens of MALT lymphoma with large cell component (high-grade MALT lymphoma), 15 specimens of diffuse large B-cell lymphoma (DLBCL), 8 specimens of complete remission of MALT lymphoma after eradication therapy, 5 specimens with no evidence of malignancy and PBMCs from 10 healthy donors. The average number of methylated genes was significantly greater in gastric lymphomas as compared to normal controls (P

Original languageEnglish
Pages (from-to)547-557
Number of pages11
JournalInternational Journal of Oncology
Volume35
Issue number3
DOIs
Publication statusPublished - 2009

Fingerprint

Marginal Zone B-Cell Lymphoma
Helicobacter Infections
Gastric Mucosa
Helicobacter pylori
Neoplasms
p16 Genes
Lymphoma, Large B-Cell, Diffuse
Gastritis
DNA Methylation
Cellular Structures
Non-Hodgkin's Lymphoma
Methylation
Genes
Tissue Donors
Polymerase Chain Reaction
DNA
Therapeutics

Keywords

  • Helicobacter pylori
  • Methylation
  • Methylation-specific PCR
  • Mucosa-associated lymphoid tissue lymphoma
  • Progression

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Accumulation of aberrant CpG hypermethylation by Helicobacter pylori infection promotes development and progression of gastric MALT lymphoma. / Kondo, Takami; Oka, Takashi; Sato, Hiaki; Shinnou, Yoko; Washio, Kana; Takano, Masayuki; Morito, Toshiaki; Takata, Katsuyoshi; Ohara, Nobuya; Oouchida, Mamoru; Shimizu, Kenji; Yoshino, Tadashi.

In: International Journal of Oncology, Vol. 35, No. 3, 2009, p. 547-557.

Research output: Contribution to journalArticle

Kondo, Takami ; Oka, Takashi ; Sato, Hiaki ; Shinnou, Yoko ; Washio, Kana ; Takano, Masayuki ; Morito, Toshiaki ; Takata, Katsuyoshi ; Ohara, Nobuya ; Oouchida, Mamoru ; Shimizu, Kenji ; Yoshino, Tadashi. / Accumulation of aberrant CpG hypermethylation by Helicobacter pylori infection promotes development and progression of gastric MALT lymphoma. In: International Journal of Oncology. 2009 ; Vol. 35, No. 3. pp. 547-557.
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