Acclimation to low [CO2] by an inorganic carbon-concentrating mechanism in Cyanophora paradoxa

S. C. Burey, V. Poroyko, Z. N. Ergen, S. Fathi-Nejad, C. Schüller, Norikazu Ohnishi, H. Fukuzawa, H. J. Bohnert, W. Löffelhardt

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The glaucocystophyte Cyanophora paradoxa contains cyanelles, plastids with prokaroytic features such as a peptidoglycan wall and a central proteinaceous inclusion body. While this central body includes the majority of the enzyme ribulose 1,5-bisphosphate carboxylase/oxgenase Rubisco), the presence of a carbon-concentrating mechanism (CCM) in C. paradoxa has only been hypothesized. Here, we present physiological data in support of a CCM: CO2 exchange activity as well as apparent affinity against inorganic carbon were found to increase under CO2-limiting stress. Further, expressed sequence tags (ESTs) of C. paradoxa were obtained from two cDNA libraries, one from cells grown in high [CO2] conditions and one from cells grown under low [CO2] conditions. A cDNA microarray platform assembled from 2378 cDNA sequences revealed that 142 genes significantly responded to a shift from high to low [CO2]. Trends in gene expression were comparable to those reported for Chlamydomonas reinhardtii and the cyanobacterium Synechocystis 6803, both possessing a CCM. Among genes regulated by [CO2], transcripts were identified encoding carbonic anhydrases (CAs), Rubisco activase and a putative bicarbonate transporter in C. paradoxa, likely functionally involved in the CCM. These results and the polyhedric appearance of the central body further support the hypothesis of a unique 'eukaryotic carboxysome' in Cyanophora.

Original languageEnglish
Pages (from-to)1422-1435
Number of pages14
JournalPlant, Cell and Environment
Volume30
Issue number11
DOIs
Publication statusPublished - Nov 2007
Externally publishedYes

Fingerprint

Cyanophora
Acclimatization
concentrating
acclimation
Carbon
carbon dioxide
carbon
Ribulose-Bisphosphate Carboxylase
Synechocystis
Chlamydomonas reinhardtii
Plastids
Carbonic Anhydrases
Peptidoglycan
Inclusion Bodies
Expressed Sequence Tags
Synechocystis sp. PCC 6803
Cyanobacteria
Tissue Plasminogen Activator
Bicarbonates
Oligonucleotide Array Sequence Analysis

Keywords

  • Carboxysome
  • cDNA library
  • Cyanelle
  • Microarrays

ASJC Scopus subject areas

  • Plant Science

Cite this

Burey, S. C., Poroyko, V., Ergen, Z. N., Fathi-Nejad, S., Schüller, C., Ohnishi, N., ... Löffelhardt, W. (2007). Acclimation to low [CO2] by an inorganic carbon-concentrating mechanism in Cyanophora paradoxa. Plant, Cell and Environment, 30(11), 1422-1435. https://doi.org/10.1111/j.1365-3040.2007.01715.x

Acclimation to low [CO2] by an inorganic carbon-concentrating mechanism in Cyanophora paradoxa. / Burey, S. C.; Poroyko, V.; Ergen, Z. N.; Fathi-Nejad, S.; Schüller, C.; Ohnishi, Norikazu; Fukuzawa, H.; Bohnert, H. J.; Löffelhardt, W.

In: Plant, Cell and Environment, Vol. 30, No. 11, 11.2007, p. 1422-1435.

Research output: Contribution to journalArticle

Burey, SC, Poroyko, V, Ergen, ZN, Fathi-Nejad, S, Schüller, C, Ohnishi, N, Fukuzawa, H, Bohnert, HJ & Löffelhardt, W 2007, 'Acclimation to low [CO2] by an inorganic carbon-concentrating mechanism in Cyanophora paradoxa', Plant, Cell and Environment, vol. 30, no. 11, pp. 1422-1435. https://doi.org/10.1111/j.1365-3040.2007.01715.x
Burey, S. C. ; Poroyko, V. ; Ergen, Z. N. ; Fathi-Nejad, S. ; Schüller, C. ; Ohnishi, Norikazu ; Fukuzawa, H. ; Bohnert, H. J. ; Löffelhardt, W. / Acclimation to low [CO2] by an inorganic carbon-concentrating mechanism in Cyanophora paradoxa. In: Plant, Cell and Environment. 2007 ; Vol. 30, No. 11. pp. 1422-1435.
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