Acceptor side effects on the electron transfer at cryogenic temperatures in intact photosystem II

Han Bao, Chunxi Zhang, Keisuke Kawakami, Yanan Ren, Jian-Ren Shen, Jingquan Zhao

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In intact PSII, both the secondary electron donor (TyrZ) and side-path electron donors (Car/ChlZ/Cytb559) can be oxidized by P680 +{radical dot} at cryogenic temperatures. In this paper, the effects of acceptor side, especially the redox state of the non-heme iron, on the donor side electron transfer induced by visible light at cryogenic temperatures were studied by EPR spectroscopy. We found that the formation and decay of the S1TyrZ{radical dot} EPR signal were independent of the treatment of K3Fe(CN)6, whereas formation and decay of the Car+{radical dot}/ChlZ +{radical dot} EPR signal correlated with the reduction and recovery of the Fe3+ EPR signal of the non-heme iron in K3Fe(CN)6 pre-treated PSII, respectively. Based on the observed correlation between Car/ChlZ oxidation and Fe3+ reduction, the oxidation of non-heme iron by K3Fe(CN)6 at 0 °C was quantified, which showed that around 50-60% fractions of the reaction centers gave rise to the Fe3+ EPR signal. In addition, we found that the presence of phenyl-p-benzoquinone significantly enhanced the yield of TyrZ oxidation. These results indicate that the electron transfer at the donor side can be significantly modified by changes at the acceptor side, and indicate that two types of reaction centers are present in intact PSII, namely, one contains unoxidizable non-heme iron and another one contains oxidizable non-heme iron. TyrZ oxidation and side-path reaction occur separately in these two types of reaction centers, instead of competition with each other in the same reaction centers. In addition, our results show that the non-heme iron has different properties in active and inactive PSII. The oxidation of non-heme iron by K3Fe(CN)6 takes place only in inactive PSII, which implies that the Fe3+ state is probably not the intermediate species for the turnover of quinone reduction.

Original languageEnglish
Pages (from-to)1109-1115
Number of pages7
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1777
Issue number9
DOIs
Publication statusPublished - Sep 2008
Externally publishedYes

Fingerprint

Photosystem II Protein Complex
Cryogenics
Iron
Electrons
Paramagnetic resonance
Temperature
Oxidation
Railroad cars
Oxidation-Reduction
Spectrum Analysis
Spectroscopy
Light
Recovery
potassium ferricyanide

Keywords

  • Electron transfer
  • EPR
  • Non-heme iron
  • Photosystem II
  • Side-path electron donor
  • Tyr

ASJC Scopus subject areas

  • Biophysics

Cite this

Acceptor side effects on the electron transfer at cryogenic temperatures in intact photosystem II. / Bao, Han; Zhang, Chunxi; Kawakami, Keisuke; Ren, Yanan; Shen, Jian-Ren; Zhao, Jingquan.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1777, No. 9, 09.2008, p. 1109-1115.

Research output: Contribution to journalArticle

Bao, Han ; Zhang, Chunxi ; Kawakami, Keisuke ; Ren, Yanan ; Shen, Jian-Ren ; Zhao, Jingquan. / Acceptor side effects on the electron transfer at cryogenic temperatures in intact photosystem II. In: Biochimica et Biophysica Acta - Bioenergetics. 2008 ; Vol. 1777, No. 9. pp. 1109-1115.
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abstract = "In intact PSII, both the secondary electron donor (TyrZ) and side-path electron donors (Car/ChlZ/Cytb559) can be oxidized by P680 +{radical dot} at cryogenic temperatures. In this paper, the effects of acceptor side, especially the redox state of the non-heme iron, on the donor side electron transfer induced by visible light at cryogenic temperatures were studied by EPR spectroscopy. We found that the formation and decay of the S1TyrZ{radical dot} EPR signal were independent of the treatment of K3Fe(CN)6, whereas formation and decay of the Car+{radical dot}/ChlZ +{radical dot} EPR signal correlated with the reduction and recovery of the Fe3+ EPR signal of the non-heme iron in K3Fe(CN)6 pre-treated PSII, respectively. Based on the observed correlation between Car/ChlZ oxidation and Fe3+ reduction, the oxidation of non-heme iron by K3Fe(CN)6 at 0 °C was quantified, which showed that around 50-60{\%} fractions of the reaction centers gave rise to the Fe3+ EPR signal. In addition, we found that the presence of phenyl-p-benzoquinone significantly enhanced the yield of TyrZ oxidation. These results indicate that the electron transfer at the donor side can be significantly modified by changes at the acceptor side, and indicate that two types of reaction centers are present in intact PSII, namely, one contains unoxidizable non-heme iron and another one contains oxidizable non-heme iron. TyrZ oxidation and side-path reaction occur separately in these two types of reaction centers, instead of competition with each other in the same reaction centers. In addition, our results show that the non-heme iron has different properties in active and inactive PSII. The oxidation of non-heme iron by K3Fe(CN)6 takes place only in inactive PSII, which implies that the Fe3+ state is probably not the intermediate species for the turnover of quinone reduction.",
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T1 - Acceptor side effects on the electron transfer at cryogenic temperatures in intact photosystem II

AU - Bao, Han

AU - Zhang, Chunxi

AU - Kawakami, Keisuke

AU - Ren, Yanan

AU - Shen, Jian-Ren

AU - Zhao, Jingquan

PY - 2008/9

Y1 - 2008/9

N2 - In intact PSII, both the secondary electron donor (TyrZ) and side-path electron donors (Car/ChlZ/Cytb559) can be oxidized by P680 +{radical dot} at cryogenic temperatures. In this paper, the effects of acceptor side, especially the redox state of the non-heme iron, on the donor side electron transfer induced by visible light at cryogenic temperatures were studied by EPR spectroscopy. We found that the formation and decay of the S1TyrZ{radical dot} EPR signal were independent of the treatment of K3Fe(CN)6, whereas formation and decay of the Car+{radical dot}/ChlZ +{radical dot} EPR signal correlated with the reduction and recovery of the Fe3+ EPR signal of the non-heme iron in K3Fe(CN)6 pre-treated PSII, respectively. Based on the observed correlation between Car/ChlZ oxidation and Fe3+ reduction, the oxidation of non-heme iron by K3Fe(CN)6 at 0 °C was quantified, which showed that around 50-60% fractions of the reaction centers gave rise to the Fe3+ EPR signal. In addition, we found that the presence of phenyl-p-benzoquinone significantly enhanced the yield of TyrZ oxidation. These results indicate that the electron transfer at the donor side can be significantly modified by changes at the acceptor side, and indicate that two types of reaction centers are present in intact PSII, namely, one contains unoxidizable non-heme iron and another one contains oxidizable non-heme iron. TyrZ oxidation and side-path reaction occur separately in these two types of reaction centers, instead of competition with each other in the same reaction centers. In addition, our results show that the non-heme iron has different properties in active and inactive PSII. The oxidation of non-heme iron by K3Fe(CN)6 takes place only in inactive PSII, which implies that the Fe3+ state is probably not the intermediate species for the turnover of quinone reduction.

AB - In intact PSII, both the secondary electron donor (TyrZ) and side-path electron donors (Car/ChlZ/Cytb559) can be oxidized by P680 +{radical dot} at cryogenic temperatures. In this paper, the effects of acceptor side, especially the redox state of the non-heme iron, on the donor side electron transfer induced by visible light at cryogenic temperatures were studied by EPR spectroscopy. We found that the formation and decay of the S1TyrZ{radical dot} EPR signal were independent of the treatment of K3Fe(CN)6, whereas formation and decay of the Car+{radical dot}/ChlZ +{radical dot} EPR signal correlated with the reduction and recovery of the Fe3+ EPR signal of the non-heme iron in K3Fe(CN)6 pre-treated PSII, respectively. Based on the observed correlation between Car/ChlZ oxidation and Fe3+ reduction, the oxidation of non-heme iron by K3Fe(CN)6 at 0 °C was quantified, which showed that around 50-60% fractions of the reaction centers gave rise to the Fe3+ EPR signal. In addition, we found that the presence of phenyl-p-benzoquinone significantly enhanced the yield of TyrZ oxidation. These results indicate that the electron transfer at the donor side can be significantly modified by changes at the acceptor side, and indicate that two types of reaction centers are present in intact PSII, namely, one contains unoxidizable non-heme iron and another one contains oxidizable non-heme iron. TyrZ oxidation and side-path reaction occur separately in these two types of reaction centers, instead of competition with each other in the same reaction centers. In addition, our results show that the non-heme iron has different properties in active and inactive PSII. The oxidation of non-heme iron by K3Fe(CN)6 takes place only in inactive PSII, which implies that the Fe3+ state is probably not the intermediate species for the turnover of quinone reduction.

KW - Electron transfer

KW - EPR

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KW - Photosystem II

KW - Side-path electron donor

KW - Tyr

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