Acceleration of bone union after structural bone grafts with a collagen-binding basic fibroblast growth factor anchored-collagen sheet for critical-size bone defects

Masaki Ueno, Kentaro Uchida, Wataru Saito, Osamu Matsushita, Mizuki Yogoro, Nozomu Nishi, Takayuki Ogura, Shunji Hattori, Gen Inoue, Keisuke Tanaka, Naonobu Takahira, Masashi Takaso

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11 Citations (Scopus)


Bone allografts are commonly used for the repair of critical-size bone defects. However, the loss of cellular activity in processed grafts markedly reduces their healing potential compared with autografts. To overcome this obstacle, we developed a healing system for critical-size bone defects that consists of overlaying an implanted bone graft with a collagen sheet (CS) loaded with basic fibroblast growth factor (bFGF) fused to the collagen-binding domain derived from a Clostridium histolyticum collagenase (CB-bFGF). In a murine femoral defect model, defect sites treated with CS/CB-bFGF had a significantly larger callus volume than those treated with CS/native bFGF. In addition, treatment with CS/CB-bFGF resulted in the rapid formation of a hard callus bridge and a larger total callus volume at the host-graft junction than treatment with CS/bFGF. Our results suggest that the combined use of CS and CB-bFGF helps accelerate the union of allogenic bone grafts.

Original languageEnglish
Article number035014
JournalBiomedical Materials (Bristol)
Issue number3
Publication statusPublished - Jun 2014



  • Basic fibroblast growth factor
  • Bone defect
  • Bone graft
  • Collagen sheet
  • Collagen-binding domain

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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