Acceleration of bone union after structural bone grafts with a collagen-binding basic fibroblast growth factor anchored-collagen sheet for critical-size bone defects

Masaki Ueno, Kentaro Uchida, Wataru Saito, Osamu Matsushita, Mizuki Yogoro, Nozomu Nishi, Takayuki Ogura, Shunji Hattori, Gen Inoue, Keisuke Tanaka, Naonobu Takahira, Masashi Takaso

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Bone allografts are commonly used for the repair of critical-size bone defects. However, the loss of cellular activity in processed grafts markedly reduces their healing potential compared with autografts. To overcome this obstacle, we developed a healing system for critical-size bone defects that consists of overlaying an implanted bone graft with a collagen sheet (CS) loaded with basic fibroblast growth factor (bFGF) fused to the collagen-binding domain derived from a Clostridium histolyticum collagenase (CB-bFGF). In a murine femoral defect model, defect sites treated with CS/CB-bFGF had a significantly larger callus volume than those treated with CS/native bFGF. In addition, treatment with CS/CB-bFGF resulted in the rapid formation of a hard callus bridge and a larger total callus volume at the host-graft junction than treatment with CS/bFGF. Our results suggest that the combined use of CS and CB-bFGF helps accelerate the union of allogenic bone grafts.

Original languageEnglish
Article number035014
JournalBiomedical Materials (Bristol)
Volume9
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Fibroblast Growth Factor 2
Fibroblasts
Collagen
Grafts
Bone
Transplants
Bone and Bones
Defects
Bony Callus
Microbial Collagenase
Clostridium
Autografts
Thigh
Intercellular Signaling Peptides and Proteins
Allografts
Repair

Keywords

  • Basic fibroblast growth factor
  • Bone defect
  • Bone graft
  • Collagen sheet
  • Collagen-binding domain

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Mechanics of Materials
  • Biochemistry
  • Medicine(all)

Cite this

Acceleration of bone union after structural bone grafts with a collagen-binding basic fibroblast growth factor anchored-collagen sheet for critical-size bone defects. / Ueno, Masaki; Uchida, Kentaro; Saito, Wataru; Matsushita, Osamu; Yogoro, Mizuki; Nishi, Nozomu; Ogura, Takayuki; Hattori, Shunji; Inoue, Gen; Tanaka, Keisuke; Takahira, Naonobu; Takaso, Masashi.

In: Biomedical Materials (Bristol), Vol. 9, No. 3, 035014, 2014.

Research output: Contribution to journalArticle

Ueno, Masaki ; Uchida, Kentaro ; Saito, Wataru ; Matsushita, Osamu ; Yogoro, Mizuki ; Nishi, Nozomu ; Ogura, Takayuki ; Hattori, Shunji ; Inoue, Gen ; Tanaka, Keisuke ; Takahira, Naonobu ; Takaso, Masashi. / Acceleration of bone union after structural bone grafts with a collagen-binding basic fibroblast growth factor anchored-collagen sheet for critical-size bone defects. In: Biomedical Materials (Bristol). 2014 ; Vol. 9, No. 3.
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