Acceleration of bone formation during fracture healing by injectable collagen powder and human basic fibroblast growth factor containing a collagen-binding domain from Clostridium histolyticum collagenase

Wataru Saito, Kentaro Uchida, Masaki Ueno, Osamu Matsushita, Gen Inoue, Nozomu Nishi, Takayuki Ogura, Shunji Hattori, Hisako Fujimaki, Keisuke Tanaka, Masashi Takaso

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

Growth factor delivered with implantable biomaterials has been used to both accelerate and ensure healing of open fractures in human patients. However, a major limitation of implantable biomaterials is the requirement for open surgical placement. Here, we developed an injectable collagen material-based bone formation system consisting of injectable collagen powder with fibril morphology and collagen triple helix conformation, and basic fibroblast growth factor (bFGF) fused to the collagen-binding domain (CBD) of Clostridium histolyticum collagenase. The affinity of the CBD towards collagen was confirmed by the results of collagen-binding assays. Moreover, the combination of the collagen binding-bFGF fusion protein (CB-bFGF) with injectable collagen powder induced bone formation at protein concentrations lower than those required for bFGF alone in mice fracture models. Taken together, these properties suggest that the CB-bFGF/collagen powder composite is a promising injectable material for bone repair in the clinical setting.

Original languageEnglish
Pages (from-to)3049-3055
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume102
Issue number9
DOIs
Publication statusPublished - Sep 2014

Keywords

  • Basic fibroblast growth factor
  • collagen-binding domain
  • collagenase
  • fracture healing
  • injectable collagen power

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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