Accelerated DNA fragmentation of the denture-bearing mucosal epithelium in an animal model of diabetes

Yukinori Maruo, T. Sugimoto, M. Oka, Tetsuya Hara, T. Sato

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study examined the effect of masticatory pressure transmitted directly to the hard palate mucosa on the final stage of terminal differentiation of keratinizing system of rats with and without streptozotocin-induced diabetes mellitus. In the nondiabetic rats with masticatory pressure, the number of terminal-deoxynucleotidyl-transferase-mediated deoxyuridine-triphospate-biotin nick end labelling (TUNEL) positive cells tended to increase about twice as much as in the nondiabetic rats without pressure with and without denture. A similar tendency of increase was observed in the diabetic rats without pressure. The synergy of the mechanical pressure and diabetic condition for 2 weeks greatly accelerated the DNA fragmentation, showing 8-fold increase in TUNEL positive cells over the normal control, and caused exfoliation of the stratum corneum. A 4-week exposure of diabetics to the masticatory pressure induced laminar splitting in the midst of the spinosum. Some cells in the stratum granulosum exhibited a sign of DNA fragmentation when laminar splitting took place in the vital cell layer. Premature DNA fragmentation may disturb the adhesion between spinosum cells and prevent the maturation of stratum corneum. Increase in Bax protein-like immunoreactivity in these epithelial cells as revealed by immunocytochemistry may underlie the premature DNA fragmentation in the oral masticatory epithelium under pressure in diabetic patients.

Original languageEnglish
Pages (from-to)393-399
Number of pages7
JournalJournal of Oral Rehabilitation
Volume28
Issue number4
Publication statusPublished - Apr 2001

Fingerprint

Dentures
DNA Fragmentation
Epithelium
Animal Models
Pressure
Deoxyuridine
Biotin
Cornea
Hard Palate
bcl-2-Associated X Protein
Experimental Diabetes Mellitus
DNA Nucleotidylexotransferase
Transferases
Diabetes Mellitus
Mucous Membrane
Epithelial Cells
Immunohistochemistry

Keywords

  • Bax protein
  • Denture base
  • DNA fragmentation
  • Masticatory pressure
  • Oral mucosa

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Accelerated DNA fragmentation of the denture-bearing mucosal epithelium in an animal model of diabetes. / Maruo, Yukinori; Sugimoto, T.; Oka, M.; Hara, Tetsuya; Sato, T.

In: Journal of Oral Rehabilitation, Vol. 28, No. 4, 04.2001, p. 393-399.

Research output: Contribution to journalArticle

@article{21416ac4a7f54a70947862080fedb941,
title = "Accelerated DNA fragmentation of the denture-bearing mucosal epithelium in an animal model of diabetes",
abstract = "This study examined the effect of masticatory pressure transmitted directly to the hard palate mucosa on the final stage of terminal differentiation of keratinizing system of rats with and without streptozotocin-induced diabetes mellitus. In the nondiabetic rats with masticatory pressure, the number of terminal-deoxynucleotidyl-transferase-mediated deoxyuridine-triphospate-biotin nick end labelling (TUNEL) positive cells tended to increase about twice as much as in the nondiabetic rats without pressure with and without denture. A similar tendency of increase was observed in the diabetic rats without pressure. The synergy of the mechanical pressure and diabetic condition for 2 weeks greatly accelerated the DNA fragmentation, showing 8-fold increase in TUNEL positive cells over the normal control, and caused exfoliation of the stratum corneum. A 4-week exposure of diabetics to the masticatory pressure induced laminar splitting in the midst of the spinosum. Some cells in the stratum granulosum exhibited a sign of DNA fragmentation when laminar splitting took place in the vital cell layer. Premature DNA fragmentation may disturb the adhesion between spinosum cells and prevent the maturation of stratum corneum. Increase in Bax protein-like immunoreactivity in these epithelial cells as revealed by immunocytochemistry may underlie the premature DNA fragmentation in the oral masticatory epithelium under pressure in diabetic patients.",
keywords = "Bax protein, Denture base, DNA fragmentation, Masticatory pressure, Oral mucosa",
author = "Yukinori Maruo and T. Sugimoto and M. Oka and Tetsuya Hara and T. Sato",
year = "2001",
month = "4",
language = "English",
volume = "28",
pages = "393--399",
journal = "Journal of Oral Rehabilitation",
issn = "0305-182X",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Accelerated DNA fragmentation of the denture-bearing mucosal epithelium in an animal model of diabetes

AU - Maruo, Yukinori

AU - Sugimoto, T.

AU - Oka, M.

AU - Hara, Tetsuya

AU - Sato, T.

PY - 2001/4

Y1 - 2001/4

N2 - This study examined the effect of masticatory pressure transmitted directly to the hard palate mucosa on the final stage of terminal differentiation of keratinizing system of rats with and without streptozotocin-induced diabetes mellitus. In the nondiabetic rats with masticatory pressure, the number of terminal-deoxynucleotidyl-transferase-mediated deoxyuridine-triphospate-biotin nick end labelling (TUNEL) positive cells tended to increase about twice as much as in the nondiabetic rats without pressure with and without denture. A similar tendency of increase was observed in the diabetic rats without pressure. The synergy of the mechanical pressure and diabetic condition for 2 weeks greatly accelerated the DNA fragmentation, showing 8-fold increase in TUNEL positive cells over the normal control, and caused exfoliation of the stratum corneum. A 4-week exposure of diabetics to the masticatory pressure induced laminar splitting in the midst of the spinosum. Some cells in the stratum granulosum exhibited a sign of DNA fragmentation when laminar splitting took place in the vital cell layer. Premature DNA fragmentation may disturb the adhesion between spinosum cells and prevent the maturation of stratum corneum. Increase in Bax protein-like immunoreactivity in these epithelial cells as revealed by immunocytochemistry may underlie the premature DNA fragmentation in the oral masticatory epithelium under pressure in diabetic patients.

AB - This study examined the effect of masticatory pressure transmitted directly to the hard palate mucosa on the final stage of terminal differentiation of keratinizing system of rats with and without streptozotocin-induced diabetes mellitus. In the nondiabetic rats with masticatory pressure, the number of terminal-deoxynucleotidyl-transferase-mediated deoxyuridine-triphospate-biotin nick end labelling (TUNEL) positive cells tended to increase about twice as much as in the nondiabetic rats without pressure with and without denture. A similar tendency of increase was observed in the diabetic rats without pressure. The synergy of the mechanical pressure and diabetic condition for 2 weeks greatly accelerated the DNA fragmentation, showing 8-fold increase in TUNEL positive cells over the normal control, and caused exfoliation of the stratum corneum. A 4-week exposure of diabetics to the masticatory pressure induced laminar splitting in the midst of the spinosum. Some cells in the stratum granulosum exhibited a sign of DNA fragmentation when laminar splitting took place in the vital cell layer. Premature DNA fragmentation may disturb the adhesion between spinosum cells and prevent the maturation of stratum corneum. Increase in Bax protein-like immunoreactivity in these epithelial cells as revealed by immunocytochemistry may underlie the premature DNA fragmentation in the oral masticatory epithelium under pressure in diabetic patients.

KW - Bax protein

KW - Denture base

KW - DNA fragmentation

KW - Masticatory pressure

KW - Oral mucosa

UR - http://www.scopus.com/inward/record.url?scp=0035316899&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035316899&partnerID=8YFLogxK

M3 - Article

C2 - 11350593

AN - SCOPUS:0035316899

VL - 28

SP - 393

EP - 399

JO - Journal of Oral Rehabilitation

JF - Journal of Oral Rehabilitation

SN - 0305-182X

IS - 4

ER -