Accelerated cell cycle progression of human regulatory T cell-like cell line caused by continuous exposure to asbestos fbers

Suni Lee, Hidenori Matsuzaki, Megumi Maeda, Shoko Yamamoto, Naoko Kumagai-Takei, Tamayo Hatayama, Miho Ikeda, Kei Yoshitome, Yasumitsu Nishimura, Takemi Otsuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Asbestos exposure causes malignant tumors such as lung cancer and malignant mesothelioma. Based on our hypothesis in which continuous exposure to asbestos of immune cells cause reduction of antitumor immunity, the decrease of natural killer cell killing activity with reduction of NKp46 activating receptor expression, inhibition of cytotoxic T cell clonal expansion, reduced CXCR3 chemokine receptor expression and production of interferon-γ production in CD4+ T cells were reported using cell line models, freshly isolated peripheral blood immune cells from health donors as well as asbestos exposed patients such as pleural plaque and mesothelioma. In addition to these fndings, regulatory T cells (Treg) showed enhanced function through cell-cell contact and increased secretion of typical soluble factors, interleukin (IL)-10 and transforming growth factor (TGF)-β, in a cell line model using the MT-2 human polyclonal T cells and its sublines exposed continuously to asbestos fbers. Since these sublines showed a remarkable reduction of FoxO1 transcription factor, which regulates various cell cycle regulators in asbestosexposed sublines, the cell cycle progression in these sublines was examined and compared with that of the original MT-2 cells. Results showed that cyclin D1 expression was markedly enhanced, and various cyclin-dependent kinase-inhibitors were reduced with increased S phases in the sublines. Furthermore, the increase of cyclin D1 expression was regulated by FoxO1. The overall findings indicate that antitumor immunity in asbestos-exposed individuals may be reduced in Treg through changes in the function and volume of Treg.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalInternational Journal of Oncology
Volume50
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Asbestos
Regulatory T-Lymphocytes
Cell Cycle
Cell Line
Cyclin D1
T-Lymphocytes
Natural Cytotoxicity Triggering Receptor 1
Immunity
CXCR3 Receptors
Cyclin-Dependent Kinases
Chemokine Receptors
Mesothelioma
Transforming Growth Factors
S Phase
Natural Killer Cells
Interleukin-10
Interferons
Lung Neoplasms
Blood Cells
Transcription Factors

Keywords

  • Asbestos
  • Cell cycle
  • Cyclin D1
  • FoxO1
  • Regulatory T cell

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Accelerated cell cycle progression of human regulatory T cell-like cell line caused by continuous exposure to asbestos fbers. / Lee, Suni; Matsuzaki, Hidenori; Maeda, Megumi; Yamamoto, Shoko; Kumagai-Takei, Naoko; Hatayama, Tamayo; Ikeda, Miho; Yoshitome, Kei; Nishimura, Yasumitsu; Otsuki, Takemi.

In: International Journal of Oncology, Vol. 50, No. 1, 01.01.2017, p. 66-74.

Research output: Contribution to journalArticle

Lee, S, Matsuzaki, H, Maeda, M, Yamamoto, S, Kumagai-Takei, N, Hatayama, T, Ikeda, M, Yoshitome, K, Nishimura, Y & Otsuki, T 2017, 'Accelerated cell cycle progression of human regulatory T cell-like cell line caused by continuous exposure to asbestos fbers', International Journal of Oncology, vol. 50, no. 1, pp. 66-74. https://doi.org/10.3892/ijo.2016.3776
Lee, Suni ; Matsuzaki, Hidenori ; Maeda, Megumi ; Yamamoto, Shoko ; Kumagai-Takei, Naoko ; Hatayama, Tamayo ; Ikeda, Miho ; Yoshitome, Kei ; Nishimura, Yasumitsu ; Otsuki, Takemi. / Accelerated cell cycle progression of human regulatory T cell-like cell line caused by continuous exposure to asbestos fbers. In: International Journal of Oncology. 2017 ; Vol. 50, No. 1. pp. 66-74.
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