Abundance of Zetaproteobacteria within crustal fluids in back-arc hydrothermal fields of the Southern Mariana Trough

Shingo Kato, Katsunori Yanagawa, Michinari Sunamura, Yoshinori Takano, Jun Ichiro Ishibashi, Takeshi Kakegawa, Motoo Utsumi, Toshiro Yamanaka, Tomohiro Toki, Takuroh Noguchi, Kensei Kobayashi, Arimichi Moroi, Hiroyuki Kimura, Yutaka Kawarabayasi, Katsumi Marumo, Tetsuro Urabe, Akihiko Yamagishi

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Abstract

Summary To extend knowledge of subseafloor microbial communities within the oceanic crust, the abundance, diversity and composition of microbial communities in crustal fluids at back-arc hydrothermal fields of the Southern Mariana Trough (SMT) were investigated using culture-independent molecular techniques based on 16S rRNA gene sequences. Seafloor drilling was carried out at two hydrothermal fields, on- and off-ridge of the back-arc spreading centre of the SMT. 16S rRNA gene clone libraries for bacterial and archaeal communities were constructed from the fluid samples collected from the boreholes. Phylotypes related to Thiomicrospira in the Gammaproteobacteria (putative sulfide-oxidizers) and Mariprofundus in the Zetaproteobacteria (putative iron-oxidizers) were recovered from the fluid samples. A number of unique archaeal phylotypes were also recovered. Fluorescence in situ hybridization (FISH) analysis indicated the presence of active bacterial and archaeal populations in the fluids. The Zetaproteobacteria accounted for up to 32% of the total prokaryotic cell number as shown by FISH analysis using a specific probe designed in this study. Our results lead to the hypothesis that the Zetaproteobacteria play a role in iron oxidation within the oceanic crust.

Original languageEnglish
Pages (from-to)3210-3222
Number of pages13
JournalEnvironmental Microbiology
Volume11
Issue number12
DOIs
Publication statusPublished - Dec 1 2009

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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    Kato, S., Yanagawa, K., Sunamura, M., Takano, Y., Ishibashi, J. I., Kakegawa, T., Utsumi, M., Yamanaka, T., Toki, T., Noguchi, T., Kobayashi, K., Moroi, A., Kimura, H., Kawarabayasi, Y., Marumo, K., Urabe, T., & Yamagishi, A. (2009). Abundance of Zetaproteobacteria within crustal fluids in back-arc hydrothermal fields of the Southern Mariana Trough. Environmental Microbiology, 11(12), 3210-3222. https://doi.org/10.1111/j.1462-2920.2009.02031.x