Absorption spectrometry using a diffractive optical element constructed by gel electrophoresis

Ayako Shigyo, Shinichi Miki, Takashi Kaneta, Totaro Imasaka

Research output: Contribution to journalArticle


A novel diffractive optical element was constructed by means of gel electrophoresis. Five capillaries were filled with a methylene blue solution and inserted into a slab gel. An electrical potential was then applied to the slab gel. Methylene Blue in the capillaries underwent electrophoretic migration, forming clear blue bands in the gel. The bands functioned as a transmission grating, and diffracted a laser beam. The intensity of the diffracted light increased with increasing the concentration of methylene blue.

Original languageEnglish
Pages (from-to)795-798
Number of pages4
JournalAnalytical Sciences
Issue number5
Publication statusPublished - May 1 2003
Externally publishedYes


ASJC Scopus subject areas

  • Analytical Chemistry

Cite this