Abstract
Head and neck squamous cell carcinoma (HNSCC) is one of the most frequently occurring types of cancer worldwide. We focused on the fact that the aberrant function of Wnt/β-catenin signaling is a frequent event in malignancies. Dickkopf (Dkk)-3 is a major negative regulator of Wnt/β catenin signaling, which is a known tumor suppressor and is down regulated in various types of cancer. However, the expression profile of the Dkk-3 protein in HNSCC has not yet been reported. The present study was conducted to investigate Dkk-3 protein expression in 90 cases of HNSCC tissue samples and HNSCC-derived cell lines. In contrast to findings available on other types of cancer, the Western blot analysis revealed that HNSCC cell lines expressed the Dkk-3 protein. In immunohistochemistry, 76 cases (84.4%) out of 90 tissue samples were Dkk-3-positive, whereas only 14 cases (15.6%) were negative. Notably, survival analysis showed that the Dkk-3 (-) group exhibited significantly longer disease-free survival (p=0.038), metastasis-free survival (p=0.013) and longer overall survival (p=0.155). The results showed that the Dkk-3 protein was dominantly expressed and may be involved in carcinogenesis and metastasis in HNSCC. Moreover, the findings suggest that the function of Dkk-3 differs depending on the tissue of origin, and that it may exert an oncogenic function in HNSCC.
| Original language | English |
|---|---|
| Pages (from-to) | 273-280 |
| Number of pages | 8 |
| Journal | Oncology Letters |
| Volume | 3 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2012 |
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Keywords
- Dkk-3
- Head and neck squamous cell carcinoma
- Immunohistochemistry
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Absence of Dickkopf (Dkk)-3 protein expression is correlated with longer disease-free survival and lower incidence of metastasis in head and neck squamous cell carcinoma. / Katase, Naoki; Lefeuvre, Mathieu; Gunduz, Mehmet; Gunduz, Esra; Beder, Levent Bekir; Grenman, Reidar; Fujii, Masae; Tamamura, Ryo; Tsujigiwa, Hidetsugu; Nagatsuka, Hitoshi.
In: Oncology Letters, Vol. 3, No. 2, 02.2012, p. 273-280.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Absence of Dickkopf (Dkk)-3 protein expression is correlated with longer disease-free survival and lower incidence of metastasis in head and neck squamous cell carcinoma
AU - Katase, Naoki
AU - Lefeuvre, Mathieu
AU - Gunduz, Mehmet
AU - Gunduz, Esra
AU - Beder, Levent Bekir
AU - Grenman, Reidar
AU - Fujii, Masae
AU - Tamamura, Ryo
AU - Tsujigiwa, Hidetsugu
AU - Nagatsuka, Hitoshi
PY - 2012/2
Y1 - 2012/2
N2 - Head and neck squamous cell carcinoma (HNSCC) is one of the most frequently occurring types of cancer worldwide. We focused on the fact that the aberrant function of Wnt/β-catenin signaling is a frequent event in malignancies. Dickkopf (Dkk)-3 is a major negative regulator of Wnt/β catenin signaling, which is a known tumor suppressor and is down regulated in various types of cancer. However, the expression profile of the Dkk-3 protein in HNSCC has not yet been reported. The present study was conducted to investigate Dkk-3 protein expression in 90 cases of HNSCC tissue samples and HNSCC-derived cell lines. In contrast to findings available on other types of cancer, the Western blot analysis revealed that HNSCC cell lines expressed the Dkk-3 protein. In immunohistochemistry, 76 cases (84.4%) out of 90 tissue samples were Dkk-3-positive, whereas only 14 cases (15.6%) were negative. Notably, survival analysis showed that the Dkk-3 (-) group exhibited significantly longer disease-free survival (p=0.038), metastasis-free survival (p=0.013) and longer overall survival (p=0.155). The results showed that the Dkk-3 protein was dominantly expressed and may be involved in carcinogenesis and metastasis in HNSCC. Moreover, the findings suggest that the function of Dkk-3 differs depending on the tissue of origin, and that it may exert an oncogenic function in HNSCC.
AB - Head and neck squamous cell carcinoma (HNSCC) is one of the most frequently occurring types of cancer worldwide. We focused on the fact that the aberrant function of Wnt/β-catenin signaling is a frequent event in malignancies. Dickkopf (Dkk)-3 is a major negative regulator of Wnt/β catenin signaling, which is a known tumor suppressor and is down regulated in various types of cancer. However, the expression profile of the Dkk-3 protein in HNSCC has not yet been reported. The present study was conducted to investigate Dkk-3 protein expression in 90 cases of HNSCC tissue samples and HNSCC-derived cell lines. In contrast to findings available on other types of cancer, the Western blot analysis revealed that HNSCC cell lines expressed the Dkk-3 protein. In immunohistochemistry, 76 cases (84.4%) out of 90 tissue samples were Dkk-3-positive, whereas only 14 cases (15.6%) were negative. Notably, survival analysis showed that the Dkk-3 (-) group exhibited significantly longer disease-free survival (p=0.038), metastasis-free survival (p=0.013) and longer overall survival (p=0.155). The results showed that the Dkk-3 protein was dominantly expressed and may be involved in carcinogenesis and metastasis in HNSCC. Moreover, the findings suggest that the function of Dkk-3 differs depending on the tissue of origin, and that it may exert an oncogenic function in HNSCC.
KW - Dkk-3
KW - Head and neck squamous cell carcinoma
KW - Immunohistochemistry
UR - http://www.scopus.com/inward/record.url?scp=84855218276&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855218276&partnerID=8YFLogxK
U2 - 10.3892/ol.2011.473
DO - 10.3892/ol.2011.473
M3 - Article
AN - SCOPUS:84855218276
VL - 3
SP - 273
EP - 280
JO - Oncology Letters
JF - Oncology Letters
SN - 1792-1074
IS - 2
ER -