Abnormalities of glycogenes in tonsillar lymphocytes in IgA nephropathy

Tatsuyuki Inoue, Hitoshi Sugiyama, Masashi Kitagawa, Keiichi Takiue, Hiroshi Morinaga, Yoko Kikumoto, Yohei Maeshima, Kunihiro Fukushima, Kazunori Nishizaki, Hirofumi Akagi, Yoshiyuki Hiki, Hirofumi Makino

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Citations (Scopus)

Abstract

Glycosylation, which represents the most complex post-translational modification, plays a pivotal role during protein maturation, and is orchestrated by numerous glycosyltransferases. Aberrant O-glycosylation of serum and tonsillar IgA1 is presumed to be one of the pathogeneses of IgA nephropathy (IgAN). However, the synthesis of underglycosylated IgA1 in tonsils has not yet been characterized. This study investigated tonsillar B lymphocytes of IgAN using tonsils from patients with chronic tonsillitis and sleep apnea syndrome. Gene expression of β1,3-galactosyltransferase (β3GalT), Cosmc, UDP-N-acetyl-α-D-galactosamine: polypeptide N-acetylgalactosaminyl- transferase 2, were significantly down regulated in tonsillar CD19-positive B lymphocytes from IgAN patients compared to control as determined by real-time RT-PCR. In contrast, the level of sialyltransferase was not significantly different among the three groups. Tonsillar B cell β3GalT gene expression significantly correlated with estimated GFR and negatively correlated with proteinuria and glomerular or interstitial injury score. Double immunofluorescent staining showed that some IgA-positive cells in the intrafollicular area were also positive for β3GalT staining. Western blotting showed the protein expression of β3GalT in the tonsils to significantly decrease in IgAN in comparison to the controls. These data suggest the downregulation of β3GalT in tonsillar B lymphocytes to be closely associated with the clinical characteristics of IgAN.

Original languageEnglish
Title of host publicationRecent Advances in Tonsils and Mucosal Barriers of the Upper Airways
Subtitle of host publication7th International Symposium on Tonsils and Mucosal Barriers of the Upper Airways, Asahikawa, July 2010: Proceedings
EditorsYasuaki Harabuchi, Tatsuya Hayashi, Akihiro Katada
Pages71-74
Number of pages4
DOIs
Publication statusPublished - Aug 2011

Publication series

NameAdvances in Oto-Rhino-Laryngology
Volume72
ISSN (Print)0065-3071

ASJC Scopus subject areas

  • Otorhinolaryngology

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