Abnormal expression of the human CD44 gene in early colorectal malignancy with special reference to variant exon 9 (9v)

K. Kawahara, T. Yoshino, N. Kawasaki, K. Miyake, T. Akagi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aims - To examine the expression of CD44 variant exon 9 in early colorectal malignancies. Methods - Formalin fixed, paraffin wax embedded tissue sections from 30 cases of tubular adenoma and 35 cases of adenoma with focal carcinoma of the colon were examined immunohistochemically using a monoclonal antibody (MAb 11.24) directed against CD44-9v. Results - In the normal colorectal mucosa immunoreactivity was confined to the basal part of the crypts and was expressed in less than 10% of crypt cells. CD44-9v was expressed in the superficial part of tubular adenoma with mild atypia in 67% of the cases and in 19% of the tumour cells. The immunoreactivity was observed along the basement-membrane in mild atypia, as in the non-neoplastic crypts. In the course of progression to severe atypia the spatial polarity of immunoreactivity was lost, and the extent of CD44-9v expression increased in intensity and in the percentage of positive cases and positive cells. In the carcinomatous lesions of adenoma with focal carcinoma, 94% of the cases and 44% of the tumour cells were positive for CD44-9v protein. Conclusion - CD44-9v may be overexpressed at the early stage of colorectal tumorigenesis and this increase continues thoughout the course of the disease.

Original languageEnglish
Pages (from-to)478-481
Number of pages4
JournalJournal of Clinical Pathology
Volume49
Issue number6
DOIs
Publication statusPublished - 1996

Keywords

  • Adenoma with focal carcinoma
  • CD44 variant exon 9
  • Colorectal carcinoma
  • Colorectal tumorigenesis
  • Immunohistochemistry
  • Tumour marker

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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