Abnormal expression of the human CD44 gene in early colorectal malignancy with special reference to variant exon 9 (9v)

K. Kawahara, Tadashi Yoshino, N. Kawasaki, K. Miyake, T. Akagi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aims - To examine the expression of CD44 variant exon 9 in early colorectal malignancies. Methods - Formalin fixed, paraffin wax embedded tissue sections from 30 cases of tubular adenoma and 35 cases of adenoma with focal carcinoma of the colon were examined immunohistochemically using a monoclonal antibody (MAb 11.24) directed against CD44-9v. Results - In the normal colorectal mucosa immunoreactivity was confined to the basal part of the crypts and was expressed in less than 10% of crypt cells. CD44-9v was expressed in the superficial part of tubular adenoma with mild atypia in 67% of the cases and in 19% of the tumour cells. The immunoreactivity was observed along the basement-membrane in mild atypia, as in the non-neoplastic crypts. In the course of progression to severe atypia the spatial polarity of immunoreactivity was lost, and the extent of CD44-9v expression increased in intensity and in the percentage of positive cases and positive cells. In the carcinomatous lesions of adenoma with focal carcinoma, 94% of the cases and 44% of the tumour cells were positive for CD44-9v protein. Conclusion - CD44-9v may be overexpressed at the early stage of colorectal tumorigenesis and this increase continues thoughout the course of the disease.

Original languageEnglish
Pages (from-to)478-481
Number of pages4
JournalJournal of Clinical Pathology
Volume49
Issue number6
Publication statusPublished - 1996

Fingerprint

Adenoma
Exons
Genes
Neoplasms
Carcinoma
Waxes
Basement Membrane
Paraffin
Formaldehyde
Colon
Carcinogenesis
Mucous Membrane
Monoclonal Antibodies
Proteins

Keywords

  • Adenoma with focal carcinoma
  • CD44 variant exon 9
  • Colorectal carcinoma
  • Colorectal tumorigenesis
  • Immunohistochemistry
  • Tumour marker

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Abnormal expression of the human CD44 gene in early colorectal malignancy with special reference to variant exon 9 (9v). / Kawahara, K.; Yoshino, Tadashi; Kawasaki, N.; Miyake, K.; Akagi, T.

In: Journal of Clinical Pathology, Vol. 49, No. 6, 1996, p. 478-481.

Research output: Contribution to journalArticle

@article{27d4af5ac2c14fc2b9319fbca9c98b93,
title = "Abnormal expression of the human CD44 gene in early colorectal malignancy with special reference to variant exon 9 (9v)",
abstract = "Aims - To examine the expression of CD44 variant exon 9 in early colorectal malignancies. Methods - Formalin fixed, paraffin wax embedded tissue sections from 30 cases of tubular adenoma and 35 cases of adenoma with focal carcinoma of the colon were examined immunohistochemically using a monoclonal antibody (MAb 11.24) directed against CD44-9v. Results - In the normal colorectal mucosa immunoreactivity was confined to the basal part of the crypts and was expressed in less than 10{\%} of crypt cells. CD44-9v was expressed in the superficial part of tubular adenoma with mild atypia in 67{\%} of the cases and in 19{\%} of the tumour cells. The immunoreactivity was observed along the basement-membrane in mild atypia, as in the non-neoplastic crypts. In the course of progression to severe atypia the spatial polarity of immunoreactivity was lost, and the extent of CD44-9v expression increased in intensity and in the percentage of positive cases and positive cells. In the carcinomatous lesions of adenoma with focal carcinoma, 94{\%} of the cases and 44{\%} of the tumour cells were positive for CD44-9v protein. Conclusion - CD44-9v may be overexpressed at the early stage of colorectal tumorigenesis and this increase continues thoughout the course of the disease.",
keywords = "Adenoma with focal carcinoma, CD44 variant exon 9, Colorectal carcinoma, Colorectal tumorigenesis, Immunohistochemistry, Tumour marker",
author = "K. Kawahara and Tadashi Yoshino and N. Kawasaki and K. Miyake and T. Akagi",
year = "1996",
language = "English",
volume = "49",
pages = "478--481",
journal = "Journal of Clinical Pathology - Clinical Molecular Pathology",
issn = "0021-9746",
publisher = "BMJ Publishing Group",
number = "6",

}

TY - JOUR

T1 - Abnormal expression of the human CD44 gene in early colorectal malignancy with special reference to variant exon 9 (9v)

AU - Kawahara, K.

AU - Yoshino, Tadashi

AU - Kawasaki, N.

AU - Miyake, K.

AU - Akagi, T.

PY - 1996

Y1 - 1996

N2 - Aims - To examine the expression of CD44 variant exon 9 in early colorectal malignancies. Methods - Formalin fixed, paraffin wax embedded tissue sections from 30 cases of tubular adenoma and 35 cases of adenoma with focal carcinoma of the colon were examined immunohistochemically using a monoclonal antibody (MAb 11.24) directed against CD44-9v. Results - In the normal colorectal mucosa immunoreactivity was confined to the basal part of the crypts and was expressed in less than 10% of crypt cells. CD44-9v was expressed in the superficial part of tubular adenoma with mild atypia in 67% of the cases and in 19% of the tumour cells. The immunoreactivity was observed along the basement-membrane in mild atypia, as in the non-neoplastic crypts. In the course of progression to severe atypia the spatial polarity of immunoreactivity was lost, and the extent of CD44-9v expression increased in intensity and in the percentage of positive cases and positive cells. In the carcinomatous lesions of adenoma with focal carcinoma, 94% of the cases and 44% of the tumour cells were positive for CD44-9v protein. Conclusion - CD44-9v may be overexpressed at the early stage of colorectal tumorigenesis and this increase continues thoughout the course of the disease.

AB - Aims - To examine the expression of CD44 variant exon 9 in early colorectal malignancies. Methods - Formalin fixed, paraffin wax embedded tissue sections from 30 cases of tubular adenoma and 35 cases of adenoma with focal carcinoma of the colon were examined immunohistochemically using a monoclonal antibody (MAb 11.24) directed against CD44-9v. Results - In the normal colorectal mucosa immunoreactivity was confined to the basal part of the crypts and was expressed in less than 10% of crypt cells. CD44-9v was expressed in the superficial part of tubular adenoma with mild atypia in 67% of the cases and in 19% of the tumour cells. The immunoreactivity was observed along the basement-membrane in mild atypia, as in the non-neoplastic crypts. In the course of progression to severe atypia the spatial polarity of immunoreactivity was lost, and the extent of CD44-9v expression increased in intensity and in the percentage of positive cases and positive cells. In the carcinomatous lesions of adenoma with focal carcinoma, 94% of the cases and 44% of the tumour cells were positive for CD44-9v protein. Conclusion - CD44-9v may be overexpressed at the early stage of colorectal tumorigenesis and this increase continues thoughout the course of the disease.

KW - Adenoma with focal carcinoma

KW - CD44 variant exon 9

KW - Colorectal carcinoma

KW - Colorectal tumorigenesis

KW - Immunohistochemistry

KW - Tumour marker

UR - http://www.scopus.com/inward/record.url?scp=0029882563&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029882563&partnerID=8YFLogxK

M3 - Article

C2 - 8763262

AN - SCOPUS:0029882563

VL - 49

SP - 478

EP - 481

JO - Journal of Clinical Pathology - Clinical Molecular Pathology

JF - Journal of Clinical Pathology - Clinical Molecular Pathology

SN - 0021-9746

IS - 6

ER -