Abnormal desmoglein expression by squamous cell carcinoma cells

Hiroshi Harada, Keiji Iwatsuki, Mikio Ohtsuka, Gang Wen Han, Fumio Kaneko

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Abnormal expression of cell adhesion molecules and related proteins has been observed in various carcinoma cells. We compared expression patterns of desmosomal cadherins, E-cadherin, and cytoplasmic plaque proteins of four different human squamous cell carcinoma cell lines and in vivo squamous cell carcinoma cells with those of normal human keratinocytes. Unlike normal human keratinocytes, the squamous cell carcinoma cells, both in culture and in vivo, exhibited diminished or unusual expression of desmoglein 3 and desmoglein 1, which bear pemphigus vulgaris and pemphigus foliaceus antigens, respectively. Abnormal expression of E-cadherin and cytoplasmic plaque proteins such as desmoplakin and plakoglobin was also observed. Western blotting study demonstrated that three squamous cell carcinoma cell lines expressed two desmogleins with a predominant 150 kDa molecule, and a minor 130 kDa one. Although these molecular sizes were similar to those of cultured normal human keratinocytes, the 130 kDa desmoglein, which usually carries pemphigus antigenic epitopes, was weakly or negatively reactive with pemphigus vulgaris serum. One squamous cell carcinoma cell line showed a doublet of 140 and 145 kDa bands in addition to the 130 kDa band. All the carcinoma cell lines constantly expressed desmoglein 2 and desmoglein 3 mRNA, whereas cultured normal human keratinocytes always expressed desmoglein 1 and desmoglein 3 mRNA, with or without desmoglein 2 mRNA. These findings indicate that the squamous cell carcinoma cells revealed abnormal expression of desmoglein isoforms, which may be related to tumour cell kinetics such as cell invasion and metastasis.

Original languageEnglish
Pages (from-to)417-420
Number of pages4
JournalActa Dermato-Venereologica
Volume76
Issue number6
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Desmogleins
Squamous Cell Carcinoma
Desmoglein 3
Desmoglein 1
Keratinocytes
Pemphigus
Desmoglein 2
Cell Line
Cadherins
Messenger RNA
Desmosomal Cadherins
Desmoplakins
gamma Catenin
Carcinoma
Proteins
Cell Adhesion Molecules
Epitopes
Protein Isoforms
Western Blotting
Neoplasm Metastasis

Keywords

  • Acantholysis
  • Cadherin
  • Cell adhesion
  • Desmosomes
  • Keratinocyte

ASJC Scopus subject areas

  • Dermatology

Cite this

Harada, H., Iwatsuki, K., Ohtsuka, M., Han, G. W., & Kaneko, F. (1996). Abnormal desmoglein expression by squamous cell carcinoma cells. Acta Dermato-Venereologica, 76(6), 417-420.

Abnormal desmoglein expression by squamous cell carcinoma cells. / Harada, Hiroshi; Iwatsuki, Keiji; Ohtsuka, Mikio; Han, Gang Wen; Kaneko, Fumio.

In: Acta Dermato-Venereologica, Vol. 76, No. 6, 1996, p. 417-420.

Research output: Contribution to journalArticle

Harada, H, Iwatsuki, K, Ohtsuka, M, Han, GW & Kaneko, F 1996, 'Abnormal desmoglein expression by squamous cell carcinoma cells', Acta Dermato-Venereologica, vol. 76, no. 6, pp. 417-420.
Harada H, Iwatsuki K, Ohtsuka M, Han GW, Kaneko F. Abnormal desmoglein expression by squamous cell carcinoma cells. Acta Dermato-Venereologica. 1996;76(6):417-420.
Harada, Hiroshi ; Iwatsuki, Keiji ; Ohtsuka, Mikio ; Han, Gang Wen ; Kaneko, Fumio. / Abnormal desmoglein expression by squamous cell carcinoma cells. In: Acta Dermato-Venereologica. 1996 ; Vol. 76, No. 6. pp. 417-420.
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