Abiotic deposition of Fe complexes onto Leptothrix sheaths

Tatsuki Kunoh, Hideki Hashimoto, Ian R. McFarlane, Naoaki Hayashi, Tomoko Suzuki, Eisuke Taketa, Katsunori Tamura, Mikio Takano, Mohamed Y. El-Naggar, Hitoshi Kunoh, Jun Takada

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Bacteria classified in species of the genus Leptothrix produce extracellular, microtubular, Fe-encrusted sheaths. The encrustation has been previously linked to bacterial Fe oxidases, which oxidize Fe(II) to Fe(III) and/or active groups of bacterial exopolymers within sheaths to attract and bind aqueous-phase inorganics. When L. cholodnii SP-6 cells were cultured in media amended with high Fe(II) concentrations, Fe(III) precipitates visibly formed immediately after addition of Fe(II) to the medium, suggesting prompt abiotic oxidation of Fe(II) to Fe(III). Intriguingly, these precipitates were deposited onto the sheath surface of bacterial cells as the population was actively growing. When Fe(III) was added to the medium, similar precipitates formed in the medium first and were abiotically deposited onto the sheath surfaces. The precipitates in the Fe(II) medium were composed of assemblies of globular, amorphous particles (ca. 50 nm diameter), while those in the Fe(III) medium were composed of large, aggregated particles (≥3 μm diameter) with a similar amorphous structure. These precipitates also adhered to cell-free sheaths. We thus concluded that direct abiotic deposition of Fe complexes onto the sheath surface occurs independently of cellular activity in liquid media containing Fe salts, although it remains unclear how this deposition is associated with the previously proposed mechanisms (oxidation enzyme- and/or active group of organic components-involved) of Fe encrustation of the Leptothrix sheaths.

Original languageEnglish
Article number26
JournalBiology
Volume5
Issue number2
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Leptothrix (bacteria)
Leptothrix
Precipitates
oxidation
Cultured Cells
Oxidoreductases
Salts
cells
Bacteria
Enzymes
Oxidation
Population
salts
liquids
bacteria
enzymes
water
Liquids

Keywords

  • Abiotic oxidation
  • Direct deposition
  • Fe(III) particles
  • Leptothrix cholodnii SP-6
  • Sheath

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Kunoh, T., Hashimoto, H., McFarlane, I. R., Hayashi, N., Suzuki, T., Taketa, E., ... Takada, J. (2016). Abiotic deposition of Fe complexes onto Leptothrix sheaths. Biology, 5(2), [26]. https://doi.org/10.3390/biology5020026

Abiotic deposition of Fe complexes onto Leptothrix sheaths. / Kunoh, Tatsuki; Hashimoto, Hideki; McFarlane, Ian R.; Hayashi, Naoaki; Suzuki, Tomoko; Taketa, Eisuke; Tamura, Katsunori; Takano, Mikio; El-Naggar, Mohamed Y.; Kunoh, Hitoshi; Takada, Jun.

In: Biology, Vol. 5, No. 2, 26, 01.06.2016.

Research output: Contribution to journalArticle

Kunoh, T, Hashimoto, H, McFarlane, IR, Hayashi, N, Suzuki, T, Taketa, E, Tamura, K, Takano, M, El-Naggar, MY, Kunoh, H & Takada, J 2016, 'Abiotic deposition of Fe complexes onto Leptothrix sheaths', Biology, vol. 5, no. 2, 26. https://doi.org/10.3390/biology5020026
Kunoh T, Hashimoto H, McFarlane IR, Hayashi N, Suzuki T, Taketa E et al. Abiotic deposition of Fe complexes onto Leptothrix sheaths. Biology. 2016 Jun 1;5(2). 26. https://doi.org/10.3390/biology5020026
Kunoh, Tatsuki ; Hashimoto, Hideki ; McFarlane, Ian R. ; Hayashi, Naoaki ; Suzuki, Tomoko ; Taketa, Eisuke ; Tamura, Katsunori ; Takano, Mikio ; El-Naggar, Mohamed Y. ; Kunoh, Hitoshi ; Takada, Jun. / Abiotic deposition of Fe complexes onto Leptothrix sheaths. In: Biology. 2016 ; Vol. 5, No. 2.
@article{d71dd57a2d0847e1886d838c5e621676,
title = "Abiotic deposition of Fe complexes onto Leptothrix sheaths",
abstract = "Bacteria classified in species of the genus Leptothrix produce extracellular, microtubular, Fe-encrusted sheaths. The encrustation has been previously linked to bacterial Fe oxidases, which oxidize Fe(II) to Fe(III) and/or active groups of bacterial exopolymers within sheaths to attract and bind aqueous-phase inorganics. When L. cholodnii SP-6 cells were cultured in media amended with high Fe(II) concentrations, Fe(III) precipitates visibly formed immediately after addition of Fe(II) to the medium, suggesting prompt abiotic oxidation of Fe(II) to Fe(III). Intriguingly, these precipitates were deposited onto the sheath surface of bacterial cells as the population was actively growing. When Fe(III) was added to the medium, similar precipitates formed in the medium first and were abiotically deposited onto the sheath surfaces. The precipitates in the Fe(II) medium were composed of assemblies of globular, amorphous particles (ca. 50 nm diameter), while those in the Fe(III) medium were composed of large, aggregated particles (≥3 μm diameter) with a similar amorphous structure. These precipitates also adhered to cell-free sheaths. We thus concluded that direct abiotic deposition of Fe complexes onto the sheath surface occurs independently of cellular activity in liquid media containing Fe salts, although it remains unclear how this deposition is associated with the previously proposed mechanisms (oxidation enzyme- and/or active group of organic components-involved) of Fe encrustation of the Leptothrix sheaths.",
keywords = "Abiotic oxidation, Direct deposition, Fe(III) particles, Leptothrix cholodnii SP-6, Sheath",
author = "Tatsuki Kunoh and Hideki Hashimoto and McFarlane, {Ian R.} and Naoaki Hayashi and Tomoko Suzuki and Eisuke Taketa and Katsunori Tamura and Mikio Takano and El-Naggar, {Mohamed Y.} and Hitoshi Kunoh and Jun Takada",
year = "2016",
month = "6",
day = "1",
doi = "10.3390/biology5020026",
language = "English",
volume = "5",
journal = "Biology",
issn = "2079-7737",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "2",

}

TY - JOUR

T1 - Abiotic deposition of Fe complexes onto Leptothrix sheaths

AU - Kunoh, Tatsuki

AU - Hashimoto, Hideki

AU - McFarlane, Ian R.

AU - Hayashi, Naoaki

AU - Suzuki, Tomoko

AU - Taketa, Eisuke

AU - Tamura, Katsunori

AU - Takano, Mikio

AU - El-Naggar, Mohamed Y.

AU - Kunoh, Hitoshi

AU - Takada, Jun

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Bacteria classified in species of the genus Leptothrix produce extracellular, microtubular, Fe-encrusted sheaths. The encrustation has been previously linked to bacterial Fe oxidases, which oxidize Fe(II) to Fe(III) and/or active groups of bacterial exopolymers within sheaths to attract and bind aqueous-phase inorganics. When L. cholodnii SP-6 cells were cultured in media amended with high Fe(II) concentrations, Fe(III) precipitates visibly formed immediately after addition of Fe(II) to the medium, suggesting prompt abiotic oxidation of Fe(II) to Fe(III). Intriguingly, these precipitates were deposited onto the sheath surface of bacterial cells as the population was actively growing. When Fe(III) was added to the medium, similar precipitates formed in the medium first and were abiotically deposited onto the sheath surfaces. The precipitates in the Fe(II) medium were composed of assemblies of globular, amorphous particles (ca. 50 nm diameter), while those in the Fe(III) medium were composed of large, aggregated particles (≥3 μm diameter) with a similar amorphous structure. These precipitates also adhered to cell-free sheaths. We thus concluded that direct abiotic deposition of Fe complexes onto the sheath surface occurs independently of cellular activity in liquid media containing Fe salts, although it remains unclear how this deposition is associated with the previously proposed mechanisms (oxidation enzyme- and/or active group of organic components-involved) of Fe encrustation of the Leptothrix sheaths.

AB - Bacteria classified in species of the genus Leptothrix produce extracellular, microtubular, Fe-encrusted sheaths. The encrustation has been previously linked to bacterial Fe oxidases, which oxidize Fe(II) to Fe(III) and/or active groups of bacterial exopolymers within sheaths to attract and bind aqueous-phase inorganics. When L. cholodnii SP-6 cells were cultured in media amended with high Fe(II) concentrations, Fe(III) precipitates visibly formed immediately after addition of Fe(II) to the medium, suggesting prompt abiotic oxidation of Fe(II) to Fe(III). Intriguingly, these precipitates were deposited onto the sheath surface of bacterial cells as the population was actively growing. When Fe(III) was added to the medium, similar precipitates formed in the medium first and were abiotically deposited onto the sheath surfaces. The precipitates in the Fe(II) medium were composed of assemblies of globular, amorphous particles (ca. 50 nm diameter), while those in the Fe(III) medium were composed of large, aggregated particles (≥3 μm diameter) with a similar amorphous structure. These precipitates also adhered to cell-free sheaths. We thus concluded that direct abiotic deposition of Fe complexes onto the sheath surface occurs independently of cellular activity in liquid media containing Fe salts, although it remains unclear how this deposition is associated with the previously proposed mechanisms (oxidation enzyme- and/or active group of organic components-involved) of Fe encrustation of the Leptothrix sheaths.

KW - Abiotic oxidation

KW - Direct deposition

KW - Fe(III) particles

KW - Leptothrix cholodnii SP-6

KW - Sheath

UR - http://www.scopus.com/inward/record.url?scp=84974533968&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84974533968&partnerID=8YFLogxK

U2 - 10.3390/biology5020026

DO - 10.3390/biology5020026

M3 - Article

VL - 5

JO - Biology

JF - Biology

SN - 2079-7737

IS - 2

M1 - 26

ER -