Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato

Yoshihiro Okabe, Tatsuya Yamaoka, Tohru Ariizumi, Koichiro Ushijima, Mikiko Kojima, Yumiko Takebayashi, Hitoshi Sakakibara, Miyako Kusano, Yoshihito Shinozaki, Sri Imriani Pulungan, Yasutaka Kubo, Ryohei Nakano, Hiroshi Ezura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Parthenocarpy, a process in which fruit set occurs without fertilization, leads to the production of seedless fruit. A number of floral homeotic mutants with abnormal stamen development exhibit parthenocarpic fruit set. Flower development is thought to repress ovary growth before anthesis. However, the mechanism of parthenocarpic fruit development caused by aberrant flower formation is poorly understood. To investigate the molecular mechanism of parthenocarpic fruit development in floral homeotic mutants, we performed functional analysis of Tomato APETALA3 (TAP3) by loss-of-function approaches. Organ-specific promoter was used to induce organ-specific loss of function in stamen and ovary/fruit. We observed increased cell expansion in tap3 mutants and TAP3-RNAi lines during parthenocarpic fruit growth. These were predominantly accompanied by the up-regulation of GA biosynthesis genes, including SlGA20ox1, SlGA20ox2, and SlGA20ox3, as well as reduced expression of the GA-inactivating gene SlGA2ox1 and the auxin signaling gene SlARF7 involved in a crosstalk between GA and auxin. These transcriptional profiles are in agreement with the GA levels in these lines. These results suggest that stamen development negatively regulates fruit set by repressing the GA biosynthesis.

Original languageEnglish
Pages (from-to)38-51
Number of pages14
JournalPlant & cell physiology
Volume60
Issue number1
DOIs
Publication statusPublished - Jan 1 2019

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Gibberellins
Lycopersicon esculentum
stamens
fruit set
gibberellins
Fruit
Up-Regulation
tomatoes
biosynthesis
mutants
fruits
fruiting
auxins
parthenocarpy
flowering
genes
Indoleacetic Acids
promoter regions
flowers
Ovary

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

Cite this

Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato. / Okabe, Yoshihiro; Yamaoka, Tatsuya; Ariizumi, Tohru; Ushijima, Koichiro; Kojima, Mikiko; Takebayashi, Yumiko; Sakakibara, Hitoshi; Kusano, Miyako; Shinozaki, Yoshihito; Pulungan, Sri Imriani; Kubo, Yasutaka; Nakano, Ryohei; Ezura, Hiroshi.

In: Plant & cell physiology, Vol. 60, No. 1, 01.01.2019, p. 38-51.

Research output: Contribution to journalArticle

Okabe, Y, Yamaoka, T, Ariizumi, T, Ushijima, K, Kojima, M, Takebayashi, Y, Sakakibara, H, Kusano, M, Shinozaki, Y, Pulungan, SI, Kubo, Y, Nakano, R & Ezura, H 2019, 'Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato', Plant & cell physiology, vol. 60, no. 1, pp. 38-51. https://doi.org/10.1093/pcp/pcy184
Okabe, Yoshihiro ; Yamaoka, Tatsuya ; Ariizumi, Tohru ; Ushijima, Koichiro ; Kojima, Mikiko ; Takebayashi, Yumiko ; Sakakibara, Hitoshi ; Kusano, Miyako ; Shinozaki, Yoshihito ; Pulungan, Sri Imriani ; Kubo, Yasutaka ; Nakano, Ryohei ; Ezura, Hiroshi. / Aberrant Stamen Development is Associated with Parthenocarpic Fruit Set Through Up-Regulation of Gibberellin Biosynthesis in Tomato. In: Plant & cell physiology. 2019 ; Vol. 60, No. 1. pp. 38-51.
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