Aberrant promoter methylation profile in pleural fluid DNA and clinicopathological factors in patients with non-small cell lung cancer

Hideki Katayama, Akio Hiraki, Keiichi Fujiwara, Keitaro Matsuo, Tadashi Maeda, Kenichi Chikamori, Daizo Kishino, Kazuo Tajima, Hiroshi Ueoka, Keisuke Aoe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The aim of this study was to investigate the prognostic value of hypermethylation of tumor suppressor genes in patients with non-small cell lung cancer (NSCLC). In samples from 34 lung patients with malignant pleural effusions, we used a methylation-specific polymerase chain reaction to detect aberrant hypermethylation of the promoters of the DNA repair gene O6-methylguanine-DNA methyltransferase (MGMT), p16INK4a, ras association domain family 1A (RASSF1A), apoptosis-related genes, death-associated protein kinase (DAPK), and retinoic acid receptor ß (RARß).There is no association between methylation status of five tumor suppressor genes including MGMT, p16INK4a, RASSF1A, DAPK and RARß in pleural fluid DNA and clinicopathological parameters including clinical outcome. Aberrant promoter methylation of tumor suppressor genes in pleural fluid DNA could not be a valuable prognostic marker of NSCLC patients with malignant pleural effusion.

Original languageEnglish
Pages (from-to)221-224
Number of pages4
JournalAsian Pacific Journal of Cancer Prevention
Volume8
Issue number2
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Non-Small Cell Lung Carcinoma
Methylation
Death-Associated Protein Kinases
Tumor Suppressor Genes
Malignant Pleural Effusion
Retinoic Acid Receptors
DNA
Methyltransferases
DNA Repair
Genes
Apoptosis
Polymerase Chain Reaction
Lung

Keywords

  • MGMT - p16ink4a - RASSF1A - DAPK - rar - Malignant Pleural Effusion - Survival

ASJC Scopus subject areas

  • Oncology
  • Cancer Research
  • Public Health, Environmental and Occupational Health
  • Epidemiology
  • Medicine(all)

Cite this

Aberrant promoter methylation profile in pleural fluid DNA and clinicopathological factors in patients with non-small cell lung cancer. / Katayama, Hideki; Hiraki, Akio; Fujiwara, Keiichi; Matsuo, Keitaro; Maeda, Tadashi; Chikamori, Kenichi; Kishino, Daizo; Tajima, Kazuo; Ueoka, Hiroshi; Aoe, Keisuke.

In: Asian Pacific Journal of Cancer Prevention, Vol. 8, No. 2, 2007, p. 221-224.

Research output: Contribution to journalArticle

Katayama, H, Hiraki, A, Fujiwara, K, Matsuo, K, Maeda, T, Chikamori, K, Kishino, D, Tajima, K, Ueoka, H & Aoe, K 2007, 'Aberrant promoter methylation profile in pleural fluid DNA and clinicopathological factors in patients with non-small cell lung cancer', Asian Pacific Journal of Cancer Prevention, vol. 8, no. 2, pp. 221-224.
Katayama, Hideki ; Hiraki, Akio ; Fujiwara, Keiichi ; Matsuo, Keitaro ; Maeda, Tadashi ; Chikamori, Kenichi ; Kishino, Daizo ; Tajima, Kazuo ; Ueoka, Hiroshi ; Aoe, Keisuke. / Aberrant promoter methylation profile in pleural fluid DNA and clinicopathological factors in patients with non-small cell lung cancer. In: Asian Pacific Journal of Cancer Prevention. 2007 ; Vol. 8, No. 2. pp. 221-224.
@article{c053d6b088ef4b2f80798fe988f80520,
title = "Aberrant promoter methylation profile in pleural fluid DNA and clinicopathological factors in patients with non-small cell lung cancer",
abstract = "The aim of this study was to investigate the prognostic value of hypermethylation of tumor suppressor genes in patients with non-small cell lung cancer (NSCLC). In samples from 34 lung patients with malignant pleural effusions, we used a methylation-specific polymerase chain reaction to detect aberrant hypermethylation of the promoters of the DNA repair gene O6-methylguanine-DNA methyltransferase (MGMT), p16INK4a, ras association domain family 1A (RASSF1A), apoptosis-related genes, death-associated protein kinase (DAPK), and retinoic acid receptor {\ss} (RAR{\ss}).There is no association between methylation status of five tumor suppressor genes including MGMT, p16INK4a, RASSF1A, DAPK and RAR{\ss} in pleural fluid DNA and clinicopathological parameters including clinical outcome. Aberrant promoter methylation of tumor suppressor genes in pleural fluid DNA could not be a valuable prognostic marker of NSCLC patients with malignant pleural effusion.",
keywords = "MGMT - p16ink4a - RASSF1A - DAPK - rar - Malignant Pleural Effusion - Survival",
author = "Hideki Katayama and Akio Hiraki and Keiichi Fujiwara and Keitaro Matsuo and Tadashi Maeda and Kenichi Chikamori and Daizo Kishino and Kazuo Tajima and Hiroshi Ueoka and Keisuke Aoe",
year = "2007",
language = "English",
volume = "8",
pages = "221--224",
journal = "Asian Pacific Journal of Cancer Prevention",
issn = "1513-7368",
publisher = "Asian Pacific Organization for Cancer Prevention",
number = "2",

}

TY - JOUR

T1 - Aberrant promoter methylation profile in pleural fluid DNA and clinicopathological factors in patients with non-small cell lung cancer

AU - Katayama, Hideki

AU - Hiraki, Akio

AU - Fujiwara, Keiichi

AU - Matsuo, Keitaro

AU - Maeda, Tadashi

AU - Chikamori, Kenichi

AU - Kishino, Daizo

AU - Tajima, Kazuo

AU - Ueoka, Hiroshi

AU - Aoe, Keisuke

PY - 2007

Y1 - 2007

N2 - The aim of this study was to investigate the prognostic value of hypermethylation of tumor suppressor genes in patients with non-small cell lung cancer (NSCLC). In samples from 34 lung patients with malignant pleural effusions, we used a methylation-specific polymerase chain reaction to detect aberrant hypermethylation of the promoters of the DNA repair gene O6-methylguanine-DNA methyltransferase (MGMT), p16INK4a, ras association domain family 1A (RASSF1A), apoptosis-related genes, death-associated protein kinase (DAPK), and retinoic acid receptor ß (RARß).There is no association between methylation status of five tumor suppressor genes including MGMT, p16INK4a, RASSF1A, DAPK and RARß in pleural fluid DNA and clinicopathological parameters including clinical outcome. Aberrant promoter methylation of tumor suppressor genes in pleural fluid DNA could not be a valuable prognostic marker of NSCLC patients with malignant pleural effusion.

AB - The aim of this study was to investigate the prognostic value of hypermethylation of tumor suppressor genes in patients with non-small cell lung cancer (NSCLC). In samples from 34 lung patients with malignant pleural effusions, we used a methylation-specific polymerase chain reaction to detect aberrant hypermethylation of the promoters of the DNA repair gene O6-methylguanine-DNA methyltransferase (MGMT), p16INK4a, ras association domain family 1A (RASSF1A), apoptosis-related genes, death-associated protein kinase (DAPK), and retinoic acid receptor ß (RARß).There is no association between methylation status of five tumor suppressor genes including MGMT, p16INK4a, RASSF1A, DAPK and RARß in pleural fluid DNA and clinicopathological parameters including clinical outcome. Aberrant promoter methylation of tumor suppressor genes in pleural fluid DNA could not be a valuable prognostic marker of NSCLC patients with malignant pleural effusion.

KW - MGMT - p16ink4a - RASSF1A - DAPK - rar - Malignant Pleural Effusion - Survival

UR - http://www.scopus.com/inward/record.url?scp=40449124506&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40449124506&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 221

EP - 224

JO - Asian Pacific Journal of Cancer Prevention

JF - Asian Pacific Journal of Cancer Prevention

SN - 1513-7368

IS - 2

ER -