Aberrant methylation and simian virus 40 tag sequences in malignant mesothelioma

Shinichi Toyooka, H. I. Pass, N. Shivapurkar, Y. Fukuyama, R. Maruyama, K. O. Toyooka, M. Gilcrease, A. Farinas, J. D. Minna, A. F. Gazdar

Research output: Contribution to journalArticle

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Abstract

Aberrant promoter methylation and resultant silencing of several genes plays an important role in the pathogenesis of many tumor types. We compared the methylation profile of 66 malignant mesotheliomas (MMs) and 40 lung adenocarcinomas using methylation-specific PCR for seven genes frequently methylated in lung cancer. We also compared the methylation frequencies of these genes as well as the methylation index, a reflection of all of the gene frequencies, with the presence of SV40 large T-antigen (Tag) sequences, histological subtype, and patient survival. Our major findings are: (a) with the exception of the RASSF1A promoter of the RASSF1 gene, frequencies of aberrant methylation were significantly lower in MMs than in adenocarcinomas; (b) the frequency of RASSF1A aberrant methylation and the value of the methylation index were significantly higher in SV40 sequence positive MM than in negative MM; and (c) the methylation index was higher in epithelial MM than in sarcomatous/mixed MM. Our results demonstrate a relationship between SV40 and aberrant methylation in MMs.

Original languageEnglish
Pages (from-to)5727-5730
Number of pages4
JournalCancer Research
Volume61
Issue number15
Publication statusPublished - Aug 1 2001
Externally publishedYes

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Simian virus 40
Methylation
Gene Frequency
Malignant Mesothelioma
Polyomavirus Transforming Antigens
Viral Tumor Antigens
Gene Silencing
Lung Neoplasms
Adenocarcinoma
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Toyooka, S., Pass, H. I., Shivapurkar, N., Fukuyama, Y., Maruyama, R., Toyooka, K. O., ... Gazdar, A. F. (2001). Aberrant methylation and simian virus 40 tag sequences in malignant mesothelioma. Cancer Research, 61(15), 5727-5730.

Aberrant methylation and simian virus 40 tag sequences in malignant mesothelioma. / Toyooka, Shinichi; Pass, H. I.; Shivapurkar, N.; Fukuyama, Y.; Maruyama, R.; Toyooka, K. O.; Gilcrease, M.; Farinas, A.; Minna, J. D.; Gazdar, A. F.

In: Cancer Research, Vol. 61, No. 15, 01.08.2001, p. 5727-5730.

Research output: Contribution to journalArticle

Toyooka, S, Pass, HI, Shivapurkar, N, Fukuyama, Y, Maruyama, R, Toyooka, KO, Gilcrease, M, Farinas, A, Minna, JD & Gazdar, AF 2001, 'Aberrant methylation and simian virus 40 tag sequences in malignant mesothelioma', Cancer Research, vol. 61, no. 15, pp. 5727-5730.
Toyooka S, Pass HI, Shivapurkar N, Fukuyama Y, Maruyama R, Toyooka KO et al. Aberrant methylation and simian virus 40 tag sequences in malignant mesothelioma. Cancer Research. 2001 Aug 1;61(15):5727-5730.
Toyooka, Shinichi ; Pass, H. I. ; Shivapurkar, N. ; Fukuyama, Y. ; Maruyama, R. ; Toyooka, K. O. ; Gilcrease, M. ; Farinas, A. ; Minna, J. D. ; Gazdar, A. F. / Aberrant methylation and simian virus 40 tag sequences in malignant mesothelioma. In: Cancer Research. 2001 ; Vol. 61, No. 15. pp. 5727-5730.
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