Aberrant expression of the monocyte/macrophage phenotype in a human T cell line immortalized by HTLV-I and an adult T cell leukemia/lymphoma cell line

H. J. Jeon, T. Akagi, Tadashi Yoshino, K. Takahashi, K. Hayashi, E. Kondo, A. B. Sarker, N. Teramoto, K. Fujiwara, N. Ohara, K. Miyamoto

Research output: Contribution to journalArticle

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Abstract

An HTLV-I-immortalized human T cell line (JP-2), a N-methyl-N'-nitro-N-nitrosoguanidine-treated JP-2 line (JP-2T), and an adult T cell leukemia cell line (ATL-1T) were examined morphologically and phenotypically. All of these cell lines expressed some T cell markers, including CD4, and showed rearrangement of T cell receptor (TCH) genes, but they lacked CD3 and TCH antigens and expressed some myelomonocytic markers (CD68, HL-21, CD15, CD16). JP-2 cells grew in suspension, but JP-2T and ATL-1T cells, which mostly adhered to the surface of culture vessels, showed macrophage-like morphological features and expressed more monocyte/macrophage markers (lysozyme, α1-antitrypsin) and fibronectin. ATL-1T cells transplanted into SCID mice lost the macrophage features. These results suggest that HTLV-I infected T cells can express some macrophage features and that these cells may provide a model that will be useful in elucidating the phenotypic variability of T cell lymphomas.

Original languageEnglish
Pages (from-to)39-48
Number of pages10
JournalPathology International
Volume44
Issue number1
Publication statusPublished - 1994

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Adult T Cell Leukemia Lymphoma
Human T-lymphotropic virus 1
Monocytes
Macrophages
T-Lymphocytes
Phenotype
Cell Line
CD3 Antigens
T-Cell Receptor Genes
Methylnitronitrosoguanidine
SCID Mice
T-Cell Lymphoma
Muramidase
Fibronectins
Suspensions

Keywords

  • cultured cells
  • histiocyte
  • HTLV-I
  • immunohistochemistry
  • leukemia/lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Aberrant expression of the monocyte/macrophage phenotype in a human T cell line immortalized by HTLV-I and an adult T cell leukemia/lymphoma cell line. / Jeon, H. J.; Akagi, T.; Yoshino, Tadashi; Takahashi, K.; Hayashi, K.; Kondo, E.; Sarker, A. B.; Teramoto, N.; Fujiwara, K.; Ohara, N.; Miyamoto, K.

In: Pathology International, Vol. 44, No. 1, 1994, p. 39-48.

Research output: Contribution to journalArticle

Jeon, HJ, Akagi, T, Yoshino, T, Takahashi, K, Hayashi, K, Kondo, E, Sarker, AB, Teramoto, N, Fujiwara, K, Ohara, N & Miyamoto, K 1994, 'Aberrant expression of the monocyte/macrophage phenotype in a human T cell line immortalized by HTLV-I and an adult T cell leukemia/lymphoma cell line', Pathology International, vol. 44, no. 1, pp. 39-48.
Jeon, H. J. ; Akagi, T. ; Yoshino, Tadashi ; Takahashi, K. ; Hayashi, K. ; Kondo, E. ; Sarker, A. B. ; Teramoto, N. ; Fujiwara, K. ; Ohara, N. ; Miyamoto, K. / Aberrant expression of the monocyte/macrophage phenotype in a human T cell line immortalized by HTLV-I and an adult T cell leukemia/lymphoma cell line. In: Pathology International. 1994 ; Vol. 44, No. 1. pp. 39-48.
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