Aberrant expression of FoxP3 in a human T cell line possessing regulatory T cell-like function and exposed continuously to asbestos fibers

Megumi Maeda, Hidenori Matsuzaki, Shoko Yamamoto, Suni Lee, Naoko Kumagai-Takei, Kei Yoshitome, Yu Min, Nagisa Sada, Yasumitsu Nishimura, Takemi Otsuki

Research output: Contribution to journalArticle

Abstract

Prompted by the known carcinogenic activity of asbestos, our investigations revealed that asbestos causes a reduction in antitumor immunity. One mechanism involves the enhancement of regulatory T (Treg) cell function and volume assayed using MT-2 original cells (Org), an HTLV-1 immortalized human T cell line which possesses Treg-like function. Continuous and relatively low-dose exposure of MT-2 to asbestos fibers yielded sublines resistant to asbestos-induced apoptosis and enhanced Treg function via cell-cell contact mechanisms and increased the production of soluble factors such as interleukin (IL)-10 and transforming growth factor (TGF)-ß. Additionally, cell cycle progression was accelerated in these sublines. Subsequently, the status of the Treg-specific transcription factor FoxP3 was examined. Unexpectedly, FoxP3 mRNA levels decreased in the sublines, although significant changes in protein expression were absent. Methylation analysis of CpG sites located in the promoter region of FoxP3 in original MT-2 cells and sublines showed almost complete methylation in Org and slight hypomethylation in the sublines. Although treatment with the demethylating agent 5-aza-deoxycytidine tended to upregulate FoxP3 expression, the methylation status did not match the mRNA expression and enhanced function. Additionally, the expression of other transcription factors related to Treg did not differ between Org and subline CB1. Collectively, aberrant expression and methylation patterns of FoxP3 were detected in human T cells continuously exposed to asbestos, although cell function was enhanced by asbestos exposure. Future analyses to identify factors responsible for Treg functional enhancements induced by asbestos, such as the investigation of surface molecules, are needed for the development of strategies to prevent the occurrence of asbestos-induced cancers.

LanguageEnglish
Pages748-758
Number of pages11
JournalOncology Reports
Volume40
Issue number2
DOIs
Publication statusPublished - Aug 1 2018

Fingerprint

Asbestos
Regulatory T-Lymphocytes
T-Lymphocytes
Cell Line
Methylation
Transcription Factors
Deoxycytidine
Messenger RNA
Human T-lymphotropic virus 1
Transforming Growth Factors
Cell Size
Genetic Promoter Regions
Interleukin-10
Immunity
Cell Cycle
Up-Regulation
Apoptosis

Keywords

  • Asbestos
  • FoxP3
  • Methylation
  • Regulatory T cell

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Aberrant expression of FoxP3 in a human T cell line possessing regulatory T cell-like function and exposed continuously to asbestos fibers. / Maeda, Megumi; Matsuzaki, Hidenori; Yamamoto, Shoko; Lee, Suni; Kumagai-Takei, Naoko; Yoshitome, Kei; Min, Yu; Sada, Nagisa; Nishimura, Yasumitsu; Otsuki, Takemi.

In: Oncology Reports, Vol. 40, No. 2, 01.08.2018, p. 748-758.

Research output: Contribution to journalArticle

Maeda, M, Matsuzaki, H, Yamamoto, S, Lee, S, Kumagai-Takei, N, Yoshitome, K, Min, Y, Sada, N, Nishimura, Y & Otsuki, T 2018, 'Aberrant expression of FoxP3 in a human T cell line possessing regulatory T cell-like function and exposed continuously to asbestos fibers' Oncology Reports, vol. 40, no. 2, pp. 748-758. https://doi.org/10.3892/or.2018.6481
Maeda, Megumi ; Matsuzaki, Hidenori ; Yamamoto, Shoko ; Lee, Suni ; Kumagai-Takei, Naoko ; Yoshitome, Kei ; Min, Yu ; Sada, Nagisa ; Nishimura, Yasumitsu ; Otsuki, Takemi. / Aberrant expression of FoxP3 in a human T cell line possessing regulatory T cell-like function and exposed continuously to asbestos fibers. In: Oncology Reports. 2018 ; Vol. 40, No. 2. pp. 748-758.
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