Aberrant differential expression of EZH1 and EZH2 in Polycomb repressive complex 2 among B- and T/NK-cell neoplasms

Lamia Abdalkader, Takashi Oka, Katsuyoshi Takata, Hiaki Sato, Ichiro Murakami, Arie P. Otte, Tadashi Yoshino

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The Polycomb repressive complex-2 members (EZH2, EED, SUZ12 and EZH1) are important regulators of haematopoiesis, cell cycle and differentiation. Over-expression of EZH2 has been linked to cancer metastases and poor prognosis. Detailed information on the expression of other members in normal and neoplastic lymphoid tissue remains to be elucidated. Immunohistochemical and immunofluorescent analyses of 156 samples from haematopoietic neoplasms patients and 27 haematopoietic cell lines were used. B-cell neoplasms showed a significant over-expression of EZH2, EED and SUZ12 in the aggressive subtypes compared to the indolent subtypes and normal tissue (p = 0.000–0.046) while expression of EZH1 was decreased in mantle cell lymphoma compared to normal tissue (p = 0.011). T/NK-cell neoplasms also showed significant over-expression of EZH2, EED and SUZ12 (p = 0.000–0.002) and decreased expression of EZH1 (p = 0.001) compared to normal cells. EZH2 and EZH1 have opposite expression patterns both in normal and neoplastic lymphoid tissues as well as an opposite relation to Ki-67. These results were supported by western blotting analyses. Immunofluorescent staining revealed a difference in the intracellular localisation of EZH1 compared to other members. These evidences suggest that EZH2 and EZH1 are important in the counter-balancing mechanisms controlling proliferation/resting of lymphoid cells. The disruption of the balanced EZH2/EZH1 ratio may play important roles in the pathogenesis of lymphomas.

Original languageEnglish
Pages (from-to)467-482
Number of pages16
JournalPathology
Volume48
Issue number5
DOIs
Publication statusPublished - Aug 1 2016

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Polycomb Repressive Complex 2
Natural Killer Cells
Lymphoid Tissue
Mantle-Cell Lymphoma
Neoplasms
Hematopoiesis
Hematologic Neoplasms
Cell Differentiation
Lymphoma
Cell Cycle
B-Lymphocytes
Western Blotting
Lymphocytes
Staining and Labeling
Neoplasm Metastasis
Cell Line

Keywords

  • EED
  • EZH1
  • EZH2
  • Polycomb group
  • SUZ12

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Aberrant differential expression of EZH1 and EZH2 in Polycomb repressive complex 2 among B- and T/NK-cell neoplasms. / Abdalkader, Lamia; Oka, Takashi; Takata, Katsuyoshi; Sato, Hiaki; Murakami, Ichiro; Otte, Arie P.; Yoshino, Tadashi.

In: Pathology, Vol. 48, No. 5, 01.08.2016, p. 467-482.

Research output: Contribution to journalArticle

Abdalkader, Lamia ; Oka, Takashi ; Takata, Katsuyoshi ; Sato, Hiaki ; Murakami, Ichiro ; Otte, Arie P. ; Yoshino, Tadashi. / Aberrant differential expression of EZH1 and EZH2 in Polycomb repressive complex 2 among B- and T/NK-cell neoplasms. In: Pathology. 2016 ; Vol. 48, No. 5. pp. 467-482.
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