Abdominal vagi mediate c-Fos expression induced by X-ray irradiation in the nucleus tractus solitarii of the rat

Yosuke Yamada, Goichi Tsukamoto, Motoi Kobashi, Akira Sasaki, Tomohiro Matsumura

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The mechanism of induction of emesis by X-ray irradiation remains largely unknown. The purpose of the present research was to clarify the neuronal basis of the induction of nausea induced by X-ray irradiation analyzing c-Fos expression in the nucleus tractus solitarii (NTS) as a marker of cellular excitation. We confirmed that the dose of X-ray irradiation (4 Gy) used for the present research could actually induce nausea by preliminary measurement of kaolin intake. Induction of c-Fos immunoreactivity in the NTS was observed in the animals that received X-ray irradiation of the whole body. The mean number of c-Fos positive cells in the animals that received irradiation was significantly larger than that in the non-irradiated animals. Partial exposure of the abdomen to X-rays showed significantly greater c-Fos expression than that of the head. These results indicated the presence of a certain route for transmitting information from the periphery toward the central nervous system by X-ray irradiation. The number of c-Fos positive cells induced by X-ray irradiation in animals vagotomized at the subdiaphragmatic level was lower than that in sham-operated animals. Animals receiving a serotonin subtype three (5-HT3, 5-hydroxytryptamine) receptor antagonist (tropisetron, ICS 205-930, 3-tropanyl-indole-3-carboxylate) showed a significant reduction in c-Fos protein expression compared to animals receiving a vehicle. These results strongly suggested that X-ray irradiation activates 5-HT3 receptors on the terminals of the abdominal vagal nerves to excite the afferent pathway, thereby inducing emesis. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalAutonomic Neuroscience: Basic and Clinical
Volume83
Issue number1-2
DOIs
Publication statusPublished - Sep 1 2000

Fingerprint

Solitary Nucleus
X-Rays
tropisetron
Nausea
Vomiting
Proto-Oncogene Proteins c-fos
Afferent Pathways
Kaolin
Receptors, Serotonin, 5-HT3
Serotonin Antagonists
Whole-Body Irradiation
Research
Abdomen
Serotonin
Central Nervous System
Head

Keywords

  • 5-hydroxytryptamine
  • c-Fos protein
  • Nucleus tractus solitarii
  • Pica
  • Vagal nerve

ASJC Scopus subject areas

  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems

Cite this

Abdominal vagi mediate c-Fos expression induced by X-ray irradiation in the nucleus tractus solitarii of the rat. / Yamada, Yosuke; Tsukamoto, Goichi; Kobashi, Motoi; Sasaki, Akira; Matsumura, Tomohiro.

In: Autonomic Neuroscience: Basic and Clinical, Vol. 83, No. 1-2, 01.09.2000, p. 29-36.

Research output: Contribution to journalArticle

@article{bbc3aa8ad6b94339acf5b41819a8d936,
title = "Abdominal vagi mediate c-Fos expression induced by X-ray irradiation in the nucleus tractus solitarii of the rat",
abstract = "The mechanism of induction of emesis by X-ray irradiation remains largely unknown. The purpose of the present research was to clarify the neuronal basis of the induction of nausea induced by X-ray irradiation analyzing c-Fos expression in the nucleus tractus solitarii (NTS) as a marker of cellular excitation. We confirmed that the dose of X-ray irradiation (4 Gy) used for the present research could actually induce nausea by preliminary measurement of kaolin intake. Induction of c-Fos immunoreactivity in the NTS was observed in the animals that received X-ray irradiation of the whole body. The mean number of c-Fos positive cells in the animals that received irradiation was significantly larger than that in the non-irradiated animals. Partial exposure of the abdomen to X-rays showed significantly greater c-Fos expression than that of the head. These results indicated the presence of a certain route for transmitting information from the periphery toward the central nervous system by X-ray irradiation. The number of c-Fos positive cells induced by X-ray irradiation in animals vagotomized at the subdiaphragmatic level was lower than that in sham-operated animals. Animals receiving a serotonin subtype three (5-HT3, 5-hydroxytryptamine) receptor antagonist (tropisetron, ICS 205-930, 3-tropanyl-indole-3-carboxylate) showed a significant reduction in c-Fos protein expression compared to animals receiving a vehicle. These results strongly suggested that X-ray irradiation activates 5-HT3 receptors on the terminals of the abdominal vagal nerves to excite the afferent pathway, thereby inducing emesis. (C) 2000 Elsevier Science B.V.",
keywords = "5-hydroxytryptamine, c-Fos protein, Nucleus tractus solitarii, Pica, Vagal nerve",
author = "Yosuke Yamada and Goichi Tsukamoto and Motoi Kobashi and Akira Sasaki and Tomohiro Matsumura",
year = "2000",
month = "9",
day = "1",
doi = "10.1016/S0165-1838(00)00105-3",
language = "English",
volume = "83",
pages = "29--36",
journal = "Autonomic Neuroscience: Basic and Clinical",
issn = "1566-0702",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Abdominal vagi mediate c-Fos expression induced by X-ray irradiation in the nucleus tractus solitarii of the rat

AU - Yamada, Yosuke

AU - Tsukamoto, Goichi

AU - Kobashi, Motoi

AU - Sasaki, Akira

AU - Matsumura, Tomohiro

PY - 2000/9/1

Y1 - 2000/9/1

N2 - The mechanism of induction of emesis by X-ray irradiation remains largely unknown. The purpose of the present research was to clarify the neuronal basis of the induction of nausea induced by X-ray irradiation analyzing c-Fos expression in the nucleus tractus solitarii (NTS) as a marker of cellular excitation. We confirmed that the dose of X-ray irradiation (4 Gy) used for the present research could actually induce nausea by preliminary measurement of kaolin intake. Induction of c-Fos immunoreactivity in the NTS was observed in the animals that received X-ray irradiation of the whole body. The mean number of c-Fos positive cells in the animals that received irradiation was significantly larger than that in the non-irradiated animals. Partial exposure of the abdomen to X-rays showed significantly greater c-Fos expression than that of the head. These results indicated the presence of a certain route for transmitting information from the periphery toward the central nervous system by X-ray irradiation. The number of c-Fos positive cells induced by X-ray irradiation in animals vagotomized at the subdiaphragmatic level was lower than that in sham-operated animals. Animals receiving a serotonin subtype three (5-HT3, 5-hydroxytryptamine) receptor antagonist (tropisetron, ICS 205-930, 3-tropanyl-indole-3-carboxylate) showed a significant reduction in c-Fos protein expression compared to animals receiving a vehicle. These results strongly suggested that X-ray irradiation activates 5-HT3 receptors on the terminals of the abdominal vagal nerves to excite the afferent pathway, thereby inducing emesis. (C) 2000 Elsevier Science B.V.

AB - The mechanism of induction of emesis by X-ray irradiation remains largely unknown. The purpose of the present research was to clarify the neuronal basis of the induction of nausea induced by X-ray irradiation analyzing c-Fos expression in the nucleus tractus solitarii (NTS) as a marker of cellular excitation. We confirmed that the dose of X-ray irradiation (4 Gy) used for the present research could actually induce nausea by preliminary measurement of kaolin intake. Induction of c-Fos immunoreactivity in the NTS was observed in the animals that received X-ray irradiation of the whole body. The mean number of c-Fos positive cells in the animals that received irradiation was significantly larger than that in the non-irradiated animals. Partial exposure of the abdomen to X-rays showed significantly greater c-Fos expression than that of the head. These results indicated the presence of a certain route for transmitting information from the periphery toward the central nervous system by X-ray irradiation. The number of c-Fos positive cells induced by X-ray irradiation in animals vagotomized at the subdiaphragmatic level was lower than that in sham-operated animals. Animals receiving a serotonin subtype three (5-HT3, 5-hydroxytryptamine) receptor antagonist (tropisetron, ICS 205-930, 3-tropanyl-indole-3-carboxylate) showed a significant reduction in c-Fos protein expression compared to animals receiving a vehicle. These results strongly suggested that X-ray irradiation activates 5-HT3 receptors on the terminals of the abdominal vagal nerves to excite the afferent pathway, thereby inducing emesis. (C) 2000 Elsevier Science B.V.

KW - 5-hydroxytryptamine

KW - c-Fos protein

KW - Nucleus tractus solitarii

KW - Pica

KW - Vagal nerve

UR - http://www.scopus.com/inward/record.url?scp=0034283368&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034283368&partnerID=8YFLogxK

U2 - 10.1016/S0165-1838(00)00105-3

DO - 10.1016/S0165-1838(00)00105-3

M3 - Article

C2 - 11023626

AN - SCOPUS:0034283368

VL - 83

SP - 29

EP - 36

JO - Autonomic Neuroscience: Basic and Clinical

JF - Autonomic Neuroscience: Basic and Clinical

SN - 1566-0702

IS - 1-2

ER -