ABC transporter AtABCG25 is involved in abscisic acid transport and responses

Takashi Kuromori, Takaaki Miyaji, Hikaru Yabuuchi, Hidetada Shimizu, Eriko Sugimoto, Asako Kamiya, Yoshinori Moriyama, Kazuo Shinozaki

Research output: Contribution to journalArticle

269 Citations (Scopus)

Abstract

Abscisic acid (ABA) is one of the most important phytohormones involved in abiotic stress responses, seed maturation, germination, and senescence. ABA is predominantly produced in vascular tissues and exerts hormonal responses in various cells, including guard cells. Although ABA responses require extrusion of ABA from ABA-producing cells in an intercellular ABA signaling pathway, the transport mechanisms of ABA through the plasma membrane remain unknown. Here we isolated an ATP-binding cassette (ABC) transporter gene, AtABCG25, from Arabidopsis by genetically screening for ABA sensitivity. AtABCG25 was expressed mainly in vascular tissues. The fluorescent protein-fused AtABCG25 was localized at the plasma membrane in plant cells. In membrane vesicles derived from AtABCG25-expressing insect cells, AtABCG25 exhibited ATP-dependent ABA transport. The AtABCG25-overexpressing plants showed higher leaf temperatures, implying an influence on stomatal regulation. These results strongly suggest that AtABCG25 is an exporter of ABA and is involved in the intercellular ABA signaling pathway. The presence of the ABA transport mechanism sheds light on the active control of multicellular ABA responses to environmental stresses among plant cells.

Original languageEnglish
Pages (from-to)2361-2366
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number5
DOIs
Publication statusPublished - Feb 2 2010

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Abscisic Acid
ATP-Binding Cassette Transporters
Plant Cells
Blood Vessels
Cell Membrane
Plant Growth Regulators
Germination
Arabidopsis
Insects
Seeds

Keywords

  • ABCG
  • Arabidopsis
  • Transposon-tagged lines

ASJC Scopus subject areas

  • General

Cite this

ABC transporter AtABCG25 is involved in abscisic acid transport and responses. / Kuromori, Takashi; Miyaji, Takaaki; Yabuuchi, Hikaru; Shimizu, Hidetada; Sugimoto, Eriko; Kamiya, Asako; Moriyama, Yoshinori; Shinozaki, Kazuo.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 5, 02.02.2010, p. 2361-2366.

Research output: Contribution to journalArticle

Kuromori, Takashi ; Miyaji, Takaaki ; Yabuuchi, Hikaru ; Shimizu, Hidetada ; Sugimoto, Eriko ; Kamiya, Asako ; Moriyama, Yoshinori ; Shinozaki, Kazuo. / ABC transporter AtABCG25 is involved in abscisic acid transport and responses. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 5. pp. 2361-2366.
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