Abstract
Aluminum (Al) toxicity is the major limiting factor of crop production on acid soils, but some plant species have evolved ways of detoxifying Al. Here, we report a C2H2-type zinc finger transcription factor ART1 (for Al resistance transcription factor 1), which specifically regulates the expression of genes related to Al tolerance in rice (Oryza sativa). ART1 is constitutively expressed in the root, and the expression level is not affected by Al treatment. ART1 is localized in the nucleus of all root cells. A yeast one-hybrid assay showed that ART1 has a transcriptional activation potential and interacts with the promoter region of STAR1, an important factor in rice Al tolerance. Microarray analysis revealed 31 downstream transcripts regulated by ART1, including STAR1 and 2 and a couple of homologs of Al tolerance genes in other plants. Some of these genes were implicated in both internal and external detoxification of Al at different cellular levels. Our findings shed light on comprehensively understanding how plants detoxify aluminum to survive in an acidic environment.
| Original language | English |
|---|---|
| Pages (from-to) | 3339-3349 |
| Number of pages | 11 |
| Journal | Plant Cell |
| Volume | 21 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 2009 |
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ASJC Scopus subject areas
- Plant Science
- Cell Biology
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A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice. / Yamaji, Naoki; Huang, Chao Feng; Nagao, Sakiko; Yano, Masahiro; Sato, Yutaka; Nagamura, Yoshiaki; Ma, Jian Feng.
In: Plant Cell, Vol. 21, No. 10, 10.2009, p. 3339-3349.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice
AU - Yamaji, Naoki
AU - Huang, Chao Feng
AU - Nagao, Sakiko
AU - Yano, Masahiro
AU - Sato, Yutaka
AU - Nagamura, Yoshiaki
AU - Ma, Jian Feng
PY - 2009/10
Y1 - 2009/10
N2 - Aluminum (Al) toxicity is the major limiting factor of crop production on acid soils, but some plant species have evolved ways of detoxifying Al. Here, we report a C2H2-type zinc finger transcription factor ART1 (for Al resistance transcription factor 1), which specifically regulates the expression of genes related to Al tolerance in rice (Oryza sativa). ART1 is constitutively expressed in the root, and the expression level is not affected by Al treatment. ART1 is localized in the nucleus of all root cells. A yeast one-hybrid assay showed that ART1 has a transcriptional activation potential and interacts with the promoter region of STAR1, an important factor in rice Al tolerance. Microarray analysis revealed 31 downstream transcripts regulated by ART1, including STAR1 and 2 and a couple of homologs of Al tolerance genes in other plants. Some of these genes were implicated in both internal and external detoxification of Al at different cellular levels. Our findings shed light on comprehensively understanding how plants detoxify aluminum to survive in an acidic environment.
AB - Aluminum (Al) toxicity is the major limiting factor of crop production on acid soils, but some plant species have evolved ways of detoxifying Al. Here, we report a C2H2-type zinc finger transcription factor ART1 (for Al resistance transcription factor 1), which specifically regulates the expression of genes related to Al tolerance in rice (Oryza sativa). ART1 is constitutively expressed in the root, and the expression level is not affected by Al treatment. ART1 is localized in the nucleus of all root cells. A yeast one-hybrid assay showed that ART1 has a transcriptional activation potential and interacts with the promoter region of STAR1, an important factor in rice Al tolerance. Microarray analysis revealed 31 downstream transcripts regulated by ART1, including STAR1 and 2 and a couple of homologs of Al tolerance genes in other plants. Some of these genes were implicated in both internal and external detoxification of Al at different cellular levels. Our findings shed light on comprehensively understanding how plants detoxify aluminum to survive in an acidic environment.
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UR - http://www.scopus.com/inward/citedby.url?scp=72049129125&partnerID=8YFLogxK
U2 - 10.1105/tpc.109.070771
DO - 10.1105/tpc.109.070771
M3 - Article
C2 - 19880795
AN - SCOPUS:72049129125
VL - 21
SP - 3339
EP - 3349
JO - Plant Cell
JF - Plant Cell
SN - 1040-4651
IS - 10
ER -