A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice

Naoki Yamaji, Chao Feng Huang, Sakiko Nagao, Masahiro Yano, Yutaka Sato, Yoshiaki Nagamura, Jian Feng Ma

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

Aluminum (Al) toxicity is the major limiting factor of crop production on acid soils, but some plant species have evolved ways of detoxifying Al. Here, we report a C2H2-type zinc finger transcription factor ART1 (for Al resistance transcription factor 1), which specifically regulates the expression of genes related to Al tolerance in rice (Oryza sativa). ART1 is constitutively expressed in the root, and the expression level is not affected by Al treatment. ART1 is localized in the nucleus of all root cells. A yeast one-hybrid assay showed that ART1 has a transcriptional activation potential and interacts with the promoter region of STAR1, an important factor in rice Al tolerance. Microarray analysis revealed 31 downstream transcripts regulated by ART1, including STAR1 and 2 and a couple of homologs of Al tolerance genes in other plants. Some of these genes were implicated in both internal and external detoxification of Al at different cellular levels. Our findings shed light on comprehensively understanding how plants detoxify aluminum to survive in an acidic environment.

Original languageEnglish
Pages (from-to)3339-3349
Number of pages11
JournalPlant Cell
Volume21
Issue number10
DOIs
Publication statusPublished - Oct 2009

Fingerprint

R Factors
zinc finger motif
Zinc Fingers
Aluminum
aluminum
Transcription Factors
transcription factors
rice
Genes
genes
Oryza
transcriptional activation
Microarray Analysis
Genetic Promoter Regions
acid soils
Transcriptional Activation
crop production
Oryza sativa
Soil
Yeasts

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice. / Yamaji, Naoki; Huang, Chao Feng; Nagao, Sakiko; Yano, Masahiro; Sato, Yutaka; Nagamura, Yoshiaki; Ma, Jian Feng.

In: Plant Cell, Vol. 21, No. 10, 10.2009, p. 3339-3349.

Research output: Contribution to journalArticle

Yamaji, Naoki ; Huang, Chao Feng ; Nagao, Sakiko ; Yano, Masahiro ; Sato, Yutaka ; Nagamura, Yoshiaki ; Ma, Jian Feng. / A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice. In: Plant Cell. 2009 ; Vol. 21, No. 10. pp. 3339-3349.
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