TY - JOUR
T1 - A Y-encoded suppressor of feminization arose via lineage-specific duplication of a cytokinin response regulator in kiwifruit
AU - Akagi, Takashi
AU - Henry, Isabelle M.
AU - Ohtani, Haruka
AU - Morimoto, Takuya
AU - Beppu, Kenji
AU - Kataoka, Ikuo
AU - Tao, Ryutaro
N1 - Funding Information:
We thank Luca Comai (UC Davis Department of Plant Biology and Genome Center) for bioinformatics support and Minori Sonoda (Graduate School of Agriculture, Kyoto University) for experimental support. A part of the plant materials of this work were originally provided from Kagawa Prefectural Agricultural Experiment Station. Some of this work was performed at the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley supported by NIH S10 OD018174 Instrumentation Grant. This work was supported by the Japan Science and Technology Agency (PRESTO to T.A.), by the Japan Society for Promotion of Science through a Grant-in-Aid for Scientific Research on Innovative Areas No. J16H06471 (to T.A.), and by a National Science Foundation IOS award under Grant 1457230 (to I.M.H.).
Publisher Copyright:
© 2018 ASPB.
PY - 2018/4
Y1 - 2018/4
N2 - Dioecy, the presence of male and female flowers on distinct individuals, has evolved independently in multiple plant lineages, and the genes involved in this differential development are just starting to be uncovered in a few species. Here, we used genomic approaches to investigate this pathway in kiwifruits (genus Actinidia). Genome-wide cataloging of male-specific subsequences, combined with transcriptome analysis, led to the identification of a type-C cytokinin response regulator as a potential sex determinant gene in this genus. Functional transgenic analyses in two model systems, Arabidopsis thaliana and Nicotiana tabacum, indicated that this gene acts as a dominant suppressor of carpel development, prompting us to name it Shy Girl (SyGI). Evolutionary analyses in a panel of Actinidia species revealed that SyGI is located in the Y-specific region of the genome and probably arose from a lineage-specific gene duplication. Comparisons with the duplicated autosomal counterpart, and with orthologs from other angiosperms, suggest that the SyGI-specific duplication and subsequent evolution of cis-elements may have played a key role in the acquisition of separate sexes in this species.
AB - Dioecy, the presence of male and female flowers on distinct individuals, has evolved independently in multiple plant lineages, and the genes involved in this differential development are just starting to be uncovered in a few species. Here, we used genomic approaches to investigate this pathway in kiwifruits (genus Actinidia). Genome-wide cataloging of male-specific subsequences, combined with transcriptome analysis, led to the identification of a type-C cytokinin response regulator as a potential sex determinant gene in this genus. Functional transgenic analyses in two model systems, Arabidopsis thaliana and Nicotiana tabacum, indicated that this gene acts as a dominant suppressor of carpel development, prompting us to name it Shy Girl (SyGI). Evolutionary analyses in a panel of Actinidia species revealed that SyGI is located in the Y-specific region of the genome and probably arose from a lineage-specific gene duplication. Comparisons with the duplicated autosomal counterpart, and with orthologs from other angiosperms, suggest that the SyGI-specific duplication and subsequent evolution of cis-elements may have played a key role in the acquisition of separate sexes in this species.
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U2 - 10.1105/tpc.17.00787
DO - 10.1105/tpc.17.00787
M3 - Article
C2 - 29626069
AN - SCOPUS:85046813595
VL - 30
SP - 780
EP - 795
JO - Plant Cell
JF - Plant Cell
SN - 1040-4651
IS - 4
ER -