Abstract
Dioecy, the presence of male and female flowers on distinct individuals, has evolved independently in multiple plant lineages, and the genes involved in this differential development are just starting to be uncovered in a few species. Here, we used genomic approaches to investigate this pathway in kiwifruits (genus Actinidia). Genome-wide cataloging of male-specific subsequences, combined with transcriptome analysis, led to the identification of a type-C cytokinin response regulator as a potential sex determinant gene in this genus. Functional transgenic analyses in two model systems, Arabidopsis thaliana and Nicotiana tabacum, indicated that this gene acts as a dominant suppressor of carpel development, prompting us to name it Shy Girl (SyGI). Evolutionary analyses in a panel of Actinidia species revealed that SyGI is located in the Y-specific region of the genome and probably arose from a lineage-specific gene duplication. Comparisons with the duplicated autosomal counterpart, and with orthologs from other angiosperms, suggest that the SyGI-specific duplication and subsequent evolution of cis-elements may have played a key role in the acquisition of separate sexes in this species.
| Original language | English |
|---|---|
| Pages (from-to) | 780-795 |
| Number of pages | 16 |
| Journal | Plant Cell |
| Volume | 30 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2018 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Plant Science
- Cell Biology
Cite this
A Y-encoded suppressor of feminization arose via lineage-specific duplication of a cytokinin response regulator in kiwifruit. / Akagi, Takashi; Henry, Isabelle M.; Ohtani, Haruka; Morimoto, Takuya; Beppu, Kenji; Kataoka, Ikuo; Tao, Ryutaro.
In: Plant Cell, Vol. 30, No. 4, 04.2018, p. 780-795.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A Y-encoded suppressor of feminization arose via lineage-specific duplication of a cytokinin response regulator in kiwifruit
AU - Akagi, Takashi
AU - Henry, Isabelle M.
AU - Ohtani, Haruka
AU - Morimoto, Takuya
AU - Beppu, Kenji
AU - Kataoka, Ikuo
AU - Tao, Ryutaro
PY - 2018/4
Y1 - 2018/4
N2 - Dioecy, the presence of male and female flowers on distinct individuals, has evolved independently in multiple plant lineages, and the genes involved in this differential development are just starting to be uncovered in a few species. Here, we used genomic approaches to investigate this pathway in kiwifruits (genus Actinidia). Genome-wide cataloging of male-specific subsequences, combined with transcriptome analysis, led to the identification of a type-C cytokinin response regulator as a potential sex determinant gene in this genus. Functional transgenic analyses in two model systems, Arabidopsis thaliana and Nicotiana tabacum, indicated that this gene acts as a dominant suppressor of carpel development, prompting us to name it Shy Girl (SyGI). Evolutionary analyses in a panel of Actinidia species revealed that SyGI is located in the Y-specific region of the genome and probably arose from a lineage-specific gene duplication. Comparisons with the duplicated autosomal counterpart, and with orthologs from other angiosperms, suggest that the SyGI-specific duplication and subsequent evolution of cis-elements may have played a key role in the acquisition of separate sexes in this species.
AB - Dioecy, the presence of male and female flowers on distinct individuals, has evolved independently in multiple plant lineages, and the genes involved in this differential development are just starting to be uncovered in a few species. Here, we used genomic approaches to investigate this pathway in kiwifruits (genus Actinidia). Genome-wide cataloging of male-specific subsequences, combined with transcriptome analysis, led to the identification of a type-C cytokinin response regulator as a potential sex determinant gene in this genus. Functional transgenic analyses in two model systems, Arabidopsis thaliana and Nicotiana tabacum, indicated that this gene acts as a dominant suppressor of carpel development, prompting us to name it Shy Girl (SyGI). Evolutionary analyses in a panel of Actinidia species revealed that SyGI is located in the Y-specific region of the genome and probably arose from a lineage-specific gene duplication. Comparisons with the duplicated autosomal counterpart, and with orthologs from other angiosperms, suggest that the SyGI-specific duplication and subsequent evolution of cis-elements may have played a key role in the acquisition of separate sexes in this species.
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U2 - 10.1105/tpc.17.00787
DO - 10.1105/tpc.17.00787
M3 - Article
C2 - 29626069
AN - SCOPUS:85046813595
VL - 30
SP - 780
EP - 795
JO - Plant Cell
JF - Plant Cell
SN - 1040-4651
IS - 4
ER -