Abstract
Seed dormancy is an adaptive mechanism and an important agronomic trait. Temperature during seed development strongly affects seed dormancy in wheat (Triticum aestivum) with lower temperatures producing higher levels of seed dormancy. To identify genes important for seed dormancy, we used a wheat microarray to analyze gene expression in embryos from mature seeds grown at lower and higher temperatures. We found that a wheat homolog of MOTHER OF FT AND TFL1 (MFT) was upregulated after physiological maturity in dormant seeds grown at the lower temperature. In situ hybridization analysis indicated that MFT was exclusively expressed in the scutellum and coleorhiza. Mapping analysis showed that MFT on chromosome 3A (MFT-3A) colocalized with the seed dormancy quantitative trait locus (QTL) QPhs.ocs-3A.1. MFT-3A expression levels in a dormant cultivar used for the detection of the QTL were higher after physiological maturity; this increased expression correlated with a single nucleotide polymorphism in the promoter region. In a complementation analysis, high levels of MFT expression were correlated with a low germination index in T1 seeds. Furthermore, precocious germination of isolated immature embryos was suppressed by transient introduction of MFT driven by the maize (Zea mays) ubiquitin promoter. Taken together, these results suggest that MFT plays an important role in the regulation of germination in wheat.
| Original language | English |
|---|---|
| Pages (from-to) | 3215-3129 |
| Number of pages | 87 |
| Journal | Plant Cell |
| Volume | 23 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sep 2011 |
Fingerprint
ASJC Scopus subject areas
- Plant Science
- Cell Biology
Cite this
A wheat homolog of MOTHER of FT and TFL1 acts in the regulation of germination. / Nakamura, Shingo; Abe, Fumitaka; Kawahigashi, Hiroyuki; Nakazono, Kou; Tagiri, Akemi; Matsumoto, Takashi; Utsugi, Shigeko; Ogawa, Taiichi; Handa, Hirokazu; Ishida, Hiroki; Mori, Masahiko; Kawaura, Kanako; Ogihara, Yasunari; Miura, Hideho.
In: Plant Cell, Vol. 23, No. 9, 09.2011, p. 3215-3129.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A wheat homolog of MOTHER of FT and TFL1 acts in the regulation of germination
AU - Nakamura, Shingo
AU - Abe, Fumitaka
AU - Kawahigashi, Hiroyuki
AU - Nakazono, Kou
AU - Tagiri, Akemi
AU - Matsumoto, Takashi
AU - Utsugi, Shigeko
AU - Ogawa, Taiichi
AU - Handa, Hirokazu
AU - Ishida, Hiroki
AU - Mori, Masahiko
AU - Kawaura, Kanako
AU - Ogihara, Yasunari
AU - Miura, Hideho
PY - 2011/9
Y1 - 2011/9
N2 - Seed dormancy is an adaptive mechanism and an important agronomic trait. Temperature during seed development strongly affects seed dormancy in wheat (Triticum aestivum) with lower temperatures producing higher levels of seed dormancy. To identify genes important for seed dormancy, we used a wheat microarray to analyze gene expression in embryos from mature seeds grown at lower and higher temperatures. We found that a wheat homolog of MOTHER OF FT AND TFL1 (MFT) was upregulated after physiological maturity in dormant seeds grown at the lower temperature. In situ hybridization analysis indicated that MFT was exclusively expressed in the scutellum and coleorhiza. Mapping analysis showed that MFT on chromosome 3A (MFT-3A) colocalized with the seed dormancy quantitative trait locus (QTL) QPhs.ocs-3A.1. MFT-3A expression levels in a dormant cultivar used for the detection of the QTL were higher after physiological maturity; this increased expression correlated with a single nucleotide polymorphism in the promoter region. In a complementation analysis, high levels of MFT expression were correlated with a low germination index in T1 seeds. Furthermore, precocious germination of isolated immature embryos was suppressed by transient introduction of MFT driven by the maize (Zea mays) ubiquitin promoter. Taken together, these results suggest that MFT plays an important role in the regulation of germination in wheat.
AB - Seed dormancy is an adaptive mechanism and an important agronomic trait. Temperature during seed development strongly affects seed dormancy in wheat (Triticum aestivum) with lower temperatures producing higher levels of seed dormancy. To identify genes important for seed dormancy, we used a wheat microarray to analyze gene expression in embryos from mature seeds grown at lower and higher temperatures. We found that a wheat homolog of MOTHER OF FT AND TFL1 (MFT) was upregulated after physiological maturity in dormant seeds grown at the lower temperature. In situ hybridization analysis indicated that MFT was exclusively expressed in the scutellum and coleorhiza. Mapping analysis showed that MFT on chromosome 3A (MFT-3A) colocalized with the seed dormancy quantitative trait locus (QTL) QPhs.ocs-3A.1. MFT-3A expression levels in a dormant cultivar used for the detection of the QTL were higher after physiological maturity; this increased expression correlated with a single nucleotide polymorphism in the promoter region. In a complementation analysis, high levels of MFT expression were correlated with a low germination index in T1 seeds. Furthermore, precocious germination of isolated immature embryos was suppressed by transient introduction of MFT driven by the maize (Zea mays) ubiquitin promoter. Taken together, these results suggest that MFT plays an important role in the regulation of germination in wheat.
UR - http://www.scopus.com/inward/record.url?scp=80055004123&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80055004123&partnerID=8YFLogxK
U2 - 10.1105/tpc.111.088492
DO - 10.1105/tpc.111.088492
M3 - Article
C2 - 21896881
AN - SCOPUS:80055004123
VL - 23
SP - 3215
EP - 3129
JO - Plant Cell
JF - Plant Cell
SN - 1040-4651
IS - 9
ER -