A wheat homolog of MOTHER of FT and TFL1 acts in the regulation of germination

Shingo Nakamura, Fumitaka Abe, Hiroyuki Kawahigashi, Kou Nakazono, Akemi Tagiri, Takashi Matsumoto, Shigeko Utsugi, Taiichi Ogawa, Hirokazu Handa, Hiroki Ishida, Masahiko Mori, Kanako Kawaura, Yasunari Ogihara, Hideho Miura

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Seed dormancy is an adaptive mechanism and an important agronomic trait. Temperature during seed development strongly affects seed dormancy in wheat (Triticum aestivum) with lower temperatures producing higher levels of seed dormancy. To identify genes important for seed dormancy, we used a wheat microarray to analyze gene expression in embryos from mature seeds grown at lower and higher temperatures. We found that a wheat homolog of MOTHER OF FT AND TFL1 (MFT) was upregulated after physiological maturity in dormant seeds grown at the lower temperature. In situ hybridization analysis indicated that MFT was exclusively expressed in the scutellum and coleorhiza. Mapping analysis showed that MFT on chromosome 3A (MFT-3A) colocalized with the seed dormancy quantitative trait locus (QTL) QPhs.ocs-3A.1. MFT-3A expression levels in a dormant cultivar used for the detection of the QTL were higher after physiological maturity; this increased expression correlated with a single nucleotide polymorphism in the promoter region. In a complementation analysis, high levels of MFT expression were correlated with a low germination index in T1 seeds. Furthermore, precocious germination of isolated immature embryos was suppressed by transient introduction of MFT driven by the maize (Zea mays) ubiquitin promoter. Taken together, these results suggest that MFT plays an important role in the regulation of germination in wheat.

Original languageEnglish
Pages (from-to)3215-3129
Number of pages87
JournalPlant Cell
Volume23
Issue number9
DOIs
Publication statusPublished - Sep 2011

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Plant Dormancy
seed dormancy
Germination
Triticum
germination
Seeds
wheat
Temperature
Quantitative Trait Loci
embryo (plant)
Zea mays
quantitative trait loci
temperature
Embryonic Structures
seeds
promoter regions
Microarray Analysis
ubiquitin
Ubiquitin
seed development

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Nakamura, S., Abe, F., Kawahigashi, H., Nakazono, K., Tagiri, A., Matsumoto, T., ... Miura, H. (2011). A wheat homolog of MOTHER of FT and TFL1 acts in the regulation of germination. Plant Cell, 23(9), 3215-3129. https://doi.org/10.1105/tpc.111.088492

A wheat homolog of MOTHER of FT and TFL1 acts in the regulation of germination. / Nakamura, Shingo; Abe, Fumitaka; Kawahigashi, Hiroyuki; Nakazono, Kou; Tagiri, Akemi; Matsumoto, Takashi; Utsugi, Shigeko; Ogawa, Taiichi; Handa, Hirokazu; Ishida, Hiroki; Mori, Masahiko; Kawaura, Kanako; Ogihara, Yasunari; Miura, Hideho.

In: Plant Cell, Vol. 23, No. 9, 09.2011, p. 3215-3129.

Research output: Contribution to journalArticle

Nakamura, S, Abe, F, Kawahigashi, H, Nakazono, K, Tagiri, A, Matsumoto, T, Utsugi, S, Ogawa, T, Handa, H, Ishida, H, Mori, M, Kawaura, K, Ogihara, Y & Miura, H 2011, 'A wheat homolog of MOTHER of FT and TFL1 acts in the regulation of germination', Plant Cell, vol. 23, no. 9, pp. 3215-3129. https://doi.org/10.1105/tpc.111.088492
Nakamura S, Abe F, Kawahigashi H, Nakazono K, Tagiri A, Matsumoto T et al. A wheat homolog of MOTHER of FT and TFL1 acts in the regulation of germination. Plant Cell. 2011 Sep;23(9):3215-3129. https://doi.org/10.1105/tpc.111.088492
Nakamura, Shingo ; Abe, Fumitaka ; Kawahigashi, Hiroyuki ; Nakazono, Kou ; Tagiri, Akemi ; Matsumoto, Takashi ; Utsugi, Shigeko ; Ogawa, Taiichi ; Handa, Hirokazu ; Ishida, Hiroki ; Mori, Masahiko ; Kawaura, Kanako ; Ogihara, Yasunari ; Miura, Hideho. / A wheat homolog of MOTHER of FT and TFL1 acts in the regulation of germination. In: Plant Cell. 2011 ; Vol. 23, No. 9. pp. 3215-3129.
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