TY - JOUR
T1 - A vertex specific dorsal selector Dve represses the ventral appendage identity in Drosophila head
AU - Kiritooshi, Naruto
AU - Yorimitsu, Takeshi
AU - Shirai, Tetsuya
AU - Puli, Oorvashi Roy
AU - Singh, Amit
AU - Nakagoshi, Hideki
N1 - Funding Information:
We are grateful to Y.H. Sun, J. Modolell, T. Tabata, S. Hayashi, T. Kojima, S. Goto, M. Sato, the Bloomington Stock Center, and the Drosophila Genetic Resource Center (DGRC) for fly strains and antibodies. We also thank the Advanced Science Research Center (Okayama University) for the use of confocal microscope OLYMPUS FV300. This work was supported by Grant-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology ( 16027233 to H.N.). A.S. is supported by NIH Grant ( 1R15 HD064557-01 ) and Ohio Cancer Research Associates seed Grant.
Publisher Copyright:
© 2014 Elsevier Ireland Ltd.
PY - 2014/8/1
Y1 - 2014/8/1
N2 - Developmental fields are subdivided into lineage-restricted cell populations, known as compartments. In the eye imaginal disc of Drosophila, dorso-ventral (DV) lineage restriction is the primary event, whereas antero-posterior compartment boundary is the first lineage restriction in other imaginal discs. The Iroquois complex (Iro- C) genes function as dorsal selectors and repress the default, ventral, identity in the eye-head primordium. In Iro- C mutant clones, change of the dorsal identity to default ventral fate leads to generation of ectopic DV boundary, which results in dorsal eye enlargement, and duplication of ventral appendages like antenna and maxillary palp. Similar phenotypes were observed in heads with defective proventriculus (dve) mutant clones. Here, we show that the homeobox gene dve is a downstream effector of Iro-C in the dorsal head capsule (vertex) specification and represses the ventral (antennal) identity. Two homeodomain proteins Distal-less (Dll) and Homothorax (Hth) are known to be determinants of the antennal identity. Ectopic antenna formation in heads with dve mutant clones was associated with ectopic Dll expression and endogenous Hth expression in the vertex region. Interestingly, dve Dll double mutant clones could also induce ectopic antennae lacking the distal structures, suggesting that the Dve activity is crucial for repressing inappropriate antenna-forming potential in the vertex region. Our results clearly indicate that not only the activation of effector genes to execute developmental program but also the repression of inappropriate program is crucial for establishment of the organ identity.
AB - Developmental fields are subdivided into lineage-restricted cell populations, known as compartments. In the eye imaginal disc of Drosophila, dorso-ventral (DV) lineage restriction is the primary event, whereas antero-posterior compartment boundary is the first lineage restriction in other imaginal discs. The Iroquois complex (Iro- C) genes function as dorsal selectors and repress the default, ventral, identity in the eye-head primordium. In Iro- C mutant clones, change of the dorsal identity to default ventral fate leads to generation of ectopic DV boundary, which results in dorsal eye enlargement, and duplication of ventral appendages like antenna and maxillary palp. Similar phenotypes were observed in heads with defective proventriculus (dve) mutant clones. Here, we show that the homeobox gene dve is a downstream effector of Iro-C in the dorsal head capsule (vertex) specification and represses the ventral (antennal) identity. Two homeodomain proteins Distal-less (Dll) and Homothorax (Hth) are known to be determinants of the antennal identity. Ectopic antenna formation in heads with dve mutant clones was associated with ectopic Dll expression and endogenous Hth expression in the vertex region. Interestingly, dve Dll double mutant clones could also induce ectopic antennae lacking the distal structures, suggesting that the Dve activity is crucial for repressing inappropriate antenna-forming potential in the vertex region. Our results clearly indicate that not only the activation of effector genes to execute developmental program but also the repression of inappropriate program is crucial for establishment of the organ identity.
KW - Antenna
KW - Dorsal
KW - Drosophila
KW - Head
KW - Ventral
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U2 - 10.1016/j.mod.2014.06.002
DO - 10.1016/j.mod.2014.06.002
M3 - Article
C2 - 24971779
AN - SCOPUS:84931360694
VL - 133
SP - 54
EP - 63
JO - Cells and Development
JF - Cells and Development
SN - 2667-291X
ER -