A variant type of Vibrio cholerae SXT element in a multidrug-resistant strain of Vibrio fluvialis

Ashraf M. Ahmed, Sumio Shinoda, Tadashi Shimamoto

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Vibrio fluvialis strain H-08942 was isolated from an infant aged 6 months who was suffering from cholera-like diarrhea in India. This strain showed the typical multidrug-resistance phenotype of an SXT element. It was resistant to sulfamethoxazole (Su), trimethoprim (Tm), chloramphenicol (Cm) and streptomycin (Sm), in addition to other antibiotics such as ampicillin (Am), furazolidone (Fz), nalidixic acid (Na), and gentamicin (Gm). The SXT element is a Vibrio cholerae-derived integrative and conjugative element (ICE) that has also been referred to as a conjugative transposon. Our goal was to find a relationship between these resistant phenotypes and the presence of the SXT element in this unique strain. By using PCR, we detected the antibiotic resistance genes, the integrase gene and the attP attachment site of SXT element. Cloning and DNA sequencing results showed that both the SXT integrase gene and its attP site of V. fluvialis were similar but not identical to those of V. cholerae. The SXT integrase gene of V. fluvialis has a 99% identity to that of V. cholerae, and the attP site of SXT of V. fluvialis is variant and shorter (641 bp) than that of V. cholerae (785 bp). It was possible for the SXT of V. fluvialis to be transferred by conjugation to a laboratory strain of Escherichia coli. Here, we report the detection of a variant SXT element in species other than V. cholerae, with molecular characterization and analysis of its integrase gene and its attP site.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalFEMS Microbiology Letters
Volume242
Issue number2
DOIs
Publication statusPublished - Jan 15 2005

Fingerprint

Vibrio cholerae
Vibrio
Integrases
Genes
Microbiological Attachment Sites
Furazolidone
Phenotype
Nalidixic Acid
Cholera
Sulfamethoxazole Drug Combination Trimethoprim
Multiple Drug Resistance
Streptomycin
Chloramphenicol
Ampicillin
Microbial Drug Resistance
Gentamicins
DNA Sequence Analysis
Organism Cloning
India
Diarrhea

Keywords

  • Multidrug resistance
  • SXT element
  • Vibrio cholerae
  • Vibrio fluvialis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

A variant type of Vibrio cholerae SXT element in a multidrug-resistant strain of Vibrio fluvialis. / Ahmed, Ashraf M.; Shinoda, Sumio; Shimamoto, Tadashi.

In: FEMS Microbiology Letters, Vol. 242, No. 2, 15.01.2005, p. 241-247.

Research output: Contribution to journalArticle

Ahmed, Ashraf M. ; Shinoda, Sumio ; Shimamoto, Tadashi. / A variant type of Vibrio cholerae SXT element in a multidrug-resistant strain of Vibrio fluvialis. In: FEMS Microbiology Letters. 2005 ; Vol. 242, No. 2. pp. 241-247.
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