A trial for a DNA diagnosis of Plasmodium vivax malaria recently reemerging in the republic of korea using microtiter plate hybridization assay

J. Y. Chai, Y. K. Park, S. M. Guk, K. H. Oh, M. D. Oh, S. H. Lee, H. S. Kim, Y. Wataya

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The polymerase chain reaction-based microtiter plate hybridization (PCR-MPH) assay was utilized for a DNA diagnosis of Plasmodium vivax malaria, which has recently reemerged in the Republic of Korea. The subjects were 18 parasite-proven patients and 5 healthy controls. Follow-up blood samples were collected from 4 patients after a standard course of treatment. Polymerase chain reaction and electrophoresis of all the patients' blood showed a prominent band at the 138 base pair area, but not in the controls or after treating the patients. Hybridization of the PCR products with known species-specific probes of the 18S rRNA of various malaria species revealed strong positive reactions against the Plasmodium vivax-specific probe (absorbance 1.30-1.90 at 405 nm) in all of the patients. The absorbance was positively correlated with the degree of blood parasitemia, but with a borderline significance. Sequencing of the probe region of the Korean P. vivax revealed no significant variations from the typical P. vivax. The results show that the PCR-MPH is a highly useful technique for the DNA diagnosis of Korean vivax malaria.

Original languageEnglish
Pages (from-to)80-84
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume63
Issue number1-2
Publication statusPublished - Dec 1 2000
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

Cite this