A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice

Chao Feng Huang, Naoki Yamaji, Zhichang Chen, Jian Feng Ma

    Research output: Contribution to journalArticlepeer-review

    161 Citations (Scopus)

    Abstract

    Toxic aluminum enters the root cells rapidly, therefore internal detoxification is required. However, the molecular mechanisms underlying this process are poorly understood. Here we functionally characterized a rice gene, Os03g0755100 (OsALS1), that is regulated by ART1, a C2H2-type zinc finger transcription factor. OsALS1 encodes a half-size ABC transporter that is a member of the TAP (transporter associated with antigen processing) sub-group. Expression of OsALS1 was rapidly and specifically induced by Al in the roots, but not by other metals or low pH. OsALS1 was localized at all cells of the roots. Furthermore, OsALS1 is localized to the tonoplast. These expression patterns and cell specificity of localization are different from those of the homologous gene AtALS1 in Arabidopsis. Knockout of OsALS1 in three independent lines resulted in significant increased sensitivity to Al, but did not affect the sensitivity to other metals and low pH. Comparison of Al accumulation patterns between wild-type and osals1 mutants showed that there was no difference in Al levels in the cell sap of root tips between wild-type and the mutants, but the mutants accumulated more Al in the cytosol and nucleus than the wild-type. Expression of OsALS1 in yeast resulted in increased Al sensitivity due to mis-localization. These results indicate that OsALS1 localized at the tonoplast is responsible for sequestration of Al into the vacuoles, which is required for internal detoxification of Al in rice.

    Original languageEnglish
    Pages (from-to)857-867
    Number of pages11
    JournalPlant Journal
    Volume69
    Issue number5
    DOIs
    Publication statusPublished - Mar 2012

    Keywords

    • ABC transporter
    • aluminum
    • detoxification
    • rice
    • vacuole

    ASJC Scopus subject areas

    • Genetics
    • Plant Science
    • Cell Biology

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