Abstract
For the first time, a sea-island cotton (Gossypium barbadense L.) thaumatin-like protein gene (GbTLP1) with a potential role in secondary cell wall development has been overexpressed in tobacco to elucidate its function. The presence of the transgene was verified by Southern blotting and higher expression levels of GbTLP1 in transgenic tobacco plants were revealed by reverse-transcription and quantitative real-time polymerase chain reaction analyses. Transgenic plants with constitutively higher expression of the GbTLP1 showed enhanced resistance against different stress agents, particularly, its performance against Verticillium dahliae was exceptional. Transgenic tobacco plants also exhibited considerable resistance against Fusarium oxysporum and some abiotic stresses including salinity and drought. In this experiment, transgenic plants without GbTLP1 expression were also used as controls, which behaved similar to non-transgenic control plants. Overexpression of GbTLP1 had no significant deleterious effect on plant growth except that flowering was delayed for 3-5 weeks. The apparent pleiotropic effect of this novel gene has given us insight to the plant defense mechanism.
| Original language | English |
|---|---|
| Pages (from-to) | 38-44 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 393 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 26 2010 |
| Externally published | Yes |
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Keywords
- Fusarium oxysporum
- GbTLP1
- Thaumatin-like protein
- Verticillium dahliae
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology
Cite this
A thaumatin-like protein gene involved in cotton fiber secondary cell wall development enhances resistance against Verticillium dahliae and other stresses in transgenic tobacco. / Munis, M. Farooq Hussain; Tu, Lili; Deng, Fenglin; Tan, Jiafu; Xu, Li; Xu, Shicheng; Long, Lu; Zhang, Xianlong.
In: Biochemical and Biophysical Research Communications, Vol. 393, No. 1, 26.02.2010, p. 38-44.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A thaumatin-like protein gene involved in cotton fiber secondary cell wall development enhances resistance against Verticillium dahliae and other stresses in transgenic tobacco
AU - Munis, M. Farooq Hussain
AU - Tu, Lili
AU - Deng, Fenglin
AU - Tan, Jiafu
AU - Xu, Li
AU - Xu, Shicheng
AU - Long, Lu
AU - Zhang, Xianlong
PY - 2010/2/26
Y1 - 2010/2/26
N2 - For the first time, a sea-island cotton (Gossypium barbadense L.) thaumatin-like protein gene (GbTLP1) with a potential role in secondary cell wall development has been overexpressed in tobacco to elucidate its function. The presence of the transgene was verified by Southern blotting and higher expression levels of GbTLP1 in transgenic tobacco plants were revealed by reverse-transcription and quantitative real-time polymerase chain reaction analyses. Transgenic plants with constitutively higher expression of the GbTLP1 showed enhanced resistance against different stress agents, particularly, its performance against Verticillium dahliae was exceptional. Transgenic tobacco plants also exhibited considerable resistance against Fusarium oxysporum and some abiotic stresses including salinity and drought. In this experiment, transgenic plants without GbTLP1 expression were also used as controls, which behaved similar to non-transgenic control plants. Overexpression of GbTLP1 had no significant deleterious effect on plant growth except that flowering was delayed for 3-5 weeks. The apparent pleiotropic effect of this novel gene has given us insight to the plant defense mechanism.
AB - For the first time, a sea-island cotton (Gossypium barbadense L.) thaumatin-like protein gene (GbTLP1) with a potential role in secondary cell wall development has been overexpressed in tobacco to elucidate its function. The presence of the transgene was verified by Southern blotting and higher expression levels of GbTLP1 in transgenic tobacco plants were revealed by reverse-transcription and quantitative real-time polymerase chain reaction analyses. Transgenic plants with constitutively higher expression of the GbTLP1 showed enhanced resistance against different stress agents, particularly, its performance against Verticillium dahliae was exceptional. Transgenic tobacco plants also exhibited considerable resistance against Fusarium oxysporum and some abiotic stresses including salinity and drought. In this experiment, transgenic plants without GbTLP1 expression were also used as controls, which behaved similar to non-transgenic control plants. Overexpression of GbTLP1 had no significant deleterious effect on plant growth except that flowering was delayed for 3-5 weeks. The apparent pleiotropic effect of this novel gene has given us insight to the plant defense mechanism.
KW - Fusarium oxysporum
KW - GbTLP1
KW - Thaumatin-like protein
KW - Verticillium dahliae
UR - http://www.scopus.com/inward/record.url?scp=77249158303&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77249158303&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2010.01.069
DO - 10.1016/j.bbrc.2010.01.069
M3 - Article
VL - 393
SP - 38
EP - 44
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -