A temperature-dependent timing mechanism is involved in the circadian system that drives locomotor rhythms in the fruit fly Drosophila melanogaster

Taishi Yoshii, Makoto Sakamoto, Kenji Tomioka

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The circadian clock of Drosophila melanogaster is thought to include rhythmic expression of period gene. Recent studies suggested, however, that a per-less oscillation is also involved in the regulation of circadian locomotor rhythms. In the present study, we examined the existence and the property of the possible per-less oscillation using arrhythmic clock mutant flies carrying per01, tim01, dClkJrk or cyc01, which lack rhythmic per expression. When temperature cycles consisting of 25°C and 30°C with various periods (T=8∼32 hr) were given, wild-type (Canton-S) flies showed locomotor rhythms entrained to temperature cycles over a wide range of period (T=8∼32 hr) in constant light (LL) while only to T=24 hr in constant darkness (DD). The mutant flies showed rhythms synchronizing with the given cycle both under LL and DD. In per01 and tim01 flies, the phase of a major peak slightly changed dependent on Ts in DD, while it did not in dClkJrk and cyc01 flies. When they were transferred from a constant temperature to a temperature cycle under DD, several cycles were necessary to establish a clear temperature entrainment in per01 and tim01 flies. These results suggest that per01 and tim01 flies have a temperature-entrainable weak oscillatory mechanism and that the per-less oscillatory mechanism may require dClk and cyc. In addition, per01 and tim01 flies changed from thermoactive in DD to cryoactive in LL, while dClkJrk and cyc01 flies did not. It is thus suggested that dClk and cyc are also involved in determining the light-associated temperature preference in per01 and tim01 flies.

Original languageEnglish
Pages (from-to)841-850
Number of pages10
JournalZoological Science
Volume19
Issue number8
DOIs
Publication statusPublished - Aug 1 2002
Externally publishedYes

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fruit flies
Drosophila melanogaster
temperature
oscillation
mutants
circadian rhythm

Keywords

  • Circadian rhythms
  • Clock mutants
  • Drosophila melanogaster
  • Per-less oscillation
  • Temperature cycle

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

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title = "A temperature-dependent timing mechanism is involved in the circadian system that drives locomotor rhythms in the fruit fly Drosophila melanogaster",
abstract = "The circadian clock of Drosophila melanogaster is thought to include rhythmic expression of period gene. Recent studies suggested, however, that a per-less oscillation is also involved in the regulation of circadian locomotor rhythms. In the present study, we examined the existence and the property of the possible per-less oscillation using arrhythmic clock mutant flies carrying per01, tim01, dClkJrk or cyc01, which lack rhythmic per expression. When temperature cycles consisting of 25°C and 30°C with various periods (T=8∼32 hr) were given, wild-type (Canton-S) flies showed locomotor rhythms entrained to temperature cycles over a wide range of period (T=8∼32 hr) in constant light (LL) while only to T=24 hr in constant darkness (DD). The mutant flies showed rhythms synchronizing with the given cycle both under LL and DD. In per01 and tim01 flies, the phase of a major peak slightly changed dependent on Ts in DD, while it did not in dClkJrk and cyc01 flies. When they were transferred from a constant temperature to a temperature cycle under DD, several cycles were necessary to establish a clear temperature entrainment in per01 and tim01 flies. These results suggest that per01 and tim01 flies have a temperature-entrainable weak oscillatory mechanism and that the per-less oscillatory mechanism may require dClk and cyc. In addition, per01 and tim01 flies changed from thermoactive in DD to cryoactive in LL, while dClkJrk and cyc01 flies did not. It is thus suggested that dClk and cyc are also involved in determining the light-associated temperature preference in per01 and tim01 flies.",
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T1 - A temperature-dependent timing mechanism is involved in the circadian system that drives locomotor rhythms in the fruit fly Drosophila melanogaster

AU - Yoshii, Taishi

AU - Sakamoto, Makoto

AU - Tomioka, Kenji

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Y1 - 2002/8/1

N2 - The circadian clock of Drosophila melanogaster is thought to include rhythmic expression of period gene. Recent studies suggested, however, that a per-less oscillation is also involved in the regulation of circadian locomotor rhythms. In the present study, we examined the existence and the property of the possible per-less oscillation using arrhythmic clock mutant flies carrying per01, tim01, dClkJrk or cyc01, which lack rhythmic per expression. When temperature cycles consisting of 25°C and 30°C with various periods (T=8∼32 hr) were given, wild-type (Canton-S) flies showed locomotor rhythms entrained to temperature cycles over a wide range of period (T=8∼32 hr) in constant light (LL) while only to T=24 hr in constant darkness (DD). The mutant flies showed rhythms synchronizing with the given cycle both under LL and DD. In per01 and tim01 flies, the phase of a major peak slightly changed dependent on Ts in DD, while it did not in dClkJrk and cyc01 flies. When they were transferred from a constant temperature to a temperature cycle under DD, several cycles were necessary to establish a clear temperature entrainment in per01 and tim01 flies. These results suggest that per01 and tim01 flies have a temperature-entrainable weak oscillatory mechanism and that the per-less oscillatory mechanism may require dClk and cyc. In addition, per01 and tim01 flies changed from thermoactive in DD to cryoactive in LL, while dClkJrk and cyc01 flies did not. It is thus suggested that dClk and cyc are also involved in determining the light-associated temperature preference in per01 and tim01 flies.

AB - The circadian clock of Drosophila melanogaster is thought to include rhythmic expression of period gene. Recent studies suggested, however, that a per-less oscillation is also involved in the regulation of circadian locomotor rhythms. In the present study, we examined the existence and the property of the possible per-less oscillation using arrhythmic clock mutant flies carrying per01, tim01, dClkJrk or cyc01, which lack rhythmic per expression. When temperature cycles consisting of 25°C and 30°C with various periods (T=8∼32 hr) were given, wild-type (Canton-S) flies showed locomotor rhythms entrained to temperature cycles over a wide range of period (T=8∼32 hr) in constant light (LL) while only to T=24 hr in constant darkness (DD). The mutant flies showed rhythms synchronizing with the given cycle both under LL and DD. In per01 and tim01 flies, the phase of a major peak slightly changed dependent on Ts in DD, while it did not in dClkJrk and cyc01 flies. When they were transferred from a constant temperature to a temperature cycle under DD, several cycles were necessary to establish a clear temperature entrainment in per01 and tim01 flies. These results suggest that per01 and tim01 flies have a temperature-entrainable weak oscillatory mechanism and that the per-less oscillatory mechanism may require dClk and cyc. In addition, per01 and tim01 flies changed from thermoactive in DD to cryoactive in LL, while dClkJrk and cyc01 flies did not. It is thus suggested that dClk and cyc are also involved in determining the light-associated temperature preference in per01 and tim01 flies.

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KW - Per-less oscillation

KW - Temperature cycle

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