A tandem repetitive sequence located in the centromeric region of common wheat (Triticum aestivum) chromosomes

M. Kishii, Kiyotaka Nagaki, H. Tsujimoto

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Although TaiI-family sequences are present in the subtelomeric region of Leymus racemosus, it became apparent in the present study that such sequences are also present in the centromeric region of common wheat (Triticum aestivum). These sequences hybridized to all chromosomes with various degrees of signal strength. FISH using TaiI and Ty3/gypsy, a conservative sequence in cereal centromeres, revealed a complicated arrangement of both sequences in all wheat chromosomes at once. Unlike the Arabidopsis centromeres characterized by massive tandem arrays of 180-bp family with flanking paracentromeric retrotransposons in all chromosomes, wheat chromosomes showed various arrangement patterns of TaiI and Ty3/gypsy sequences depending on the chromosome; TaiI-family sequences were scattered in many wheat centromeres as isolated colonies instead of forming uninterrupted solid tandem arrays. This pattern may have resulted from retrotransposon insertion within pre-existing TaiI-tandem arrays or a two-step amplification mechanism of the TaiI family where each TaiI colony was amplified to form arrays independently after the insertion of TaiI-family sequences along the entire centromere. Although sequence analysis of centromeric TaiI repeats in wheat and subtelomeric TaiI repeats in L. racemosus showed variable and conservative regions between the two repeats, they did not show a distinctive difference phylogenically. The widespread presence of tandem repetitive sequences in the eucaryotic centromere suggests a significant role for them in centromeric formation.

Original languageEnglish
Pages (from-to)417-428
Number of pages12
JournalChromosome Research
Volume9
Issue number5
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Nucleic Acid Repetitive Sequences
Centromere
Triticum
Chromosomes
Retroelements
Arabidopsis
Sequence Analysis

Keywords

  • Centromere
  • Common wheat
  • TaiI family
  • Tandem repetitive sequences

ASJC Scopus subject areas

  • Genetics

Cite this

A tandem repetitive sequence located in the centromeric region of common wheat (Triticum aestivum) chromosomes. / Kishii, M.; Nagaki, Kiyotaka; Tsujimoto, H.

In: Chromosome Research, Vol. 9, No. 5, 2001, p. 417-428.

Research output: Contribution to journalArticle

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