Abstract
Field-collected US strain C18 of Cryphonectria parasitica, the chestnut blight fungus, was earlier reported to be infected by a double-stranded RNA virus, mycoreovirus 2 (MyRV2). Next-generation sequencing has revealed co-infection of C18 by a positive-strand RNA virus, hypovirus 4 (CHV4). The current molecular and genetic analyses showed interesting commensal interactions between the two viruses. CHV4 facilitated the stable infection and enhanced vertical transmission of MyRV2, which was readily lost during subculturing and showed reduced vertical transmission in single infections. Deletion of a key antiviral RNA silencing gene, dcl2, in isolate C18 increased stability of MyRV2 in single infections. The ability of CHV4 to facilitate stable infection with MyRV2 appears to be associated with the inhibitory effect of CHV4 on RNA silencing via compromising the induction of transcriptional up-regulation of dcl2. These results suggest that natural infection of isolate C18 by MyRV2 in the field was facilitated by CHV4 co-infection.
| Original language | English |
|---|---|
| Pages (from-to) | 99-107 |
| Number of pages | 9 |
| Journal | Virology |
| Volume | 533 |
| DOIs | |
| Publication status | Published - Jul 1 2019 |
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Keywords
- Chestnut blight fungus
- Co-infection
- Commensalism
- Cryphonectria parasitica
- Dicer
- Hypovirus
- Mycovirus
- Reovirus
- RNA silencing
ASJC Scopus subject areas
- Virology
Cite this
A symptomless hypovirus, CHV4, facilitates stable infection of the chestnut blight fungus by a coinfecting reovirus likely through suppression of antiviral RNA silencing. / Aulia, Annisa; Ida Bagus, Andika; Kondo, Hideki; Hillman, Bradley I.; Suzuki, Nobuhiro.
In: Virology, Vol. 533, 01.07.2019, p. 99-107.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A symptomless hypovirus, CHV4, facilitates stable infection of the chestnut blight fungus by a coinfecting reovirus likely through suppression of antiviral RNA silencing
AU - Aulia, Annisa
AU - Ida Bagus, Andika
AU - Kondo, Hideki
AU - Hillman, Bradley I.
AU - Suzuki, Nobuhiro
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Field-collected US strain C18 of Cryphonectria parasitica, the chestnut blight fungus, was earlier reported to be infected by a double-stranded RNA virus, mycoreovirus 2 (MyRV2). Next-generation sequencing has revealed co-infection of C18 by a positive-strand RNA virus, hypovirus 4 (CHV4). The current molecular and genetic analyses showed interesting commensal interactions between the two viruses. CHV4 facilitated the stable infection and enhanced vertical transmission of MyRV2, which was readily lost during subculturing and showed reduced vertical transmission in single infections. Deletion of a key antiviral RNA silencing gene, dcl2, in isolate C18 increased stability of MyRV2 in single infections. The ability of CHV4 to facilitate stable infection with MyRV2 appears to be associated with the inhibitory effect of CHV4 on RNA silencing via compromising the induction of transcriptional up-regulation of dcl2. These results suggest that natural infection of isolate C18 by MyRV2 in the field was facilitated by CHV4 co-infection.
AB - Field-collected US strain C18 of Cryphonectria parasitica, the chestnut blight fungus, was earlier reported to be infected by a double-stranded RNA virus, mycoreovirus 2 (MyRV2). Next-generation sequencing has revealed co-infection of C18 by a positive-strand RNA virus, hypovirus 4 (CHV4). The current molecular and genetic analyses showed interesting commensal interactions between the two viruses. CHV4 facilitated the stable infection and enhanced vertical transmission of MyRV2, which was readily lost during subculturing and showed reduced vertical transmission in single infections. Deletion of a key antiviral RNA silencing gene, dcl2, in isolate C18 increased stability of MyRV2 in single infections. The ability of CHV4 to facilitate stable infection with MyRV2 appears to be associated with the inhibitory effect of CHV4 on RNA silencing via compromising the induction of transcriptional up-regulation of dcl2. These results suggest that natural infection of isolate C18 by MyRV2 in the field was facilitated by CHV4 co-infection.
KW - Chestnut blight fungus
KW - Co-infection
KW - Commensalism
KW - Cryphonectria parasitica
KW - Dicer
KW - Hypovirus
KW - Mycovirus
KW - Reovirus
KW - RNA silencing
UR - http://www.scopus.com/inward/record.url?scp=85066429428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85066429428&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2019.05.004
DO - 10.1016/j.virol.2019.05.004
M3 - Article
VL - 533
SP - 99
EP - 107
JO - Virology
JF - Virology
SN - 0042-6822
ER -