A symptomless hypovirus, CHV4, facilitates stable infection of the chestnut blight fungus by a coinfecting reovirus likely through suppression of antiviral RNA silencing

Annisa Aulia, Ida Bagus Andika, Hideki Kondo, Bradley I. Hillman, Nobuhiro Suzuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Field-collected US strain C18 of Cryphonectria parasitica, the chestnut blight fungus, was earlier reported to be infected by a double-stranded RNA virus, mycoreovirus 2 (MyRV2). Next-generation sequencing has revealed co-infection of C18 by a positive-strand RNA virus, hypovirus 4 (CHV4). The current molecular and genetic analyses showed interesting commensal interactions between the two viruses. CHV4 facilitated the stable infection and enhanced vertical transmission of MyRV2, which was readily lost during subculturing and showed reduced vertical transmission in single infections. Deletion of a key antiviral RNA silencing gene, dcl2, in isolate C18 increased stability of MyRV2 in single infections. The ability of CHV4 to facilitate stable infection with MyRV2 appears to be associated with the inhibitory effect of CHV4 on RNA silencing via compromising the induction of transcriptional up-regulation of dcl2. These results suggest that natural infection of isolate C18 by MyRV2 in the field was facilitated by CHV4 co-infection.

Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalVirology
Volume533
DOIs
Publication statusPublished - Jul 2019

Keywords

  • Chestnut blight fungus
  • Co-infection
  • Commensalism
  • Cryphonectria parasitica
  • Dicer
  • Hypovirus
  • Mycovirus
  • RNA silencing
  • Reovirus

ASJC Scopus subject areas

  • Virology

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