A survey of the values of clinical chemistry parameters obtained for a common rat blood sample in ninety-eight Japanese laboratories

Toshiaki Matsuzawa, Yutaka Hayashi, Mamoru Nomura, Takashi Unno, Toshiji Igarashi, Tsuyoshi Furuya, Kiyoshi Sekita, Atsushi Ono, Yuji Kurokawa, Yuzo Hayashi

Research output: Contribution to journalArticle

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Abstract

A control survey was conducted to check the accuracy of automated analyzers used in the evaluation of clinical chemistry parameters in nonclinical toxicology studies. Pooled serum samples from male Sprague- Dawley rats were delivered refrigerated to each facility 98 laboratory facilities throughout Japan within 18 hours after sample preparation and analyzed. Commercially available normal human serum samples from a single lot were also analyzed at the same time. Survey results were divided into three categories. (1) Parameters with small coefficient of variation (CV) values for both rat and human serum samples included protein, glucose, cholesterol (CHO), urea nitrogen (UN), sodium (Na), potassium (K), chloride (Cl), calcium (Ca), and inorganic phosphate (IP). Definition of normal values in rats should be straight forward for these parameters. (2) Parameters with large CV values, but with a relatively good correlation between rat and human values include triglycerides (TG), glutamic oxaloacetic transaminase/aspartate aminotransferase (GOT/AST), glutamic pyruvic transaminase/alanine aminotransferase (GPT/ALT), and alkaline phosphatase (ALP). Measurements based on different principles gave different mean values, and this values contributed to the increase in CV values. Assessment of normal values would require a consideration of the measurement principles. (3) Parameters with large CV values only in rat serum samples included albumin (albumin/globulin ratio: A/G ratio), creatinine (CRE), and total bilirubin(BIL). Reactivity was different in rat albumin (ALB), depending on the reagents used. This difference needs to be corrected with values available by electrophoresis, or adjusted by rat ALB values, because of the lack of an appropriate measurement method. The enzyme method gave low values for rat CRE, which suggests the need for further examination of this method. The BIL values were extremely low in rat samples. It seems to be necessary to select appropriate methods to measure clinical pathology parameters correctly for rats. There was no deviation in values due solely to the mechanical operations of the analytical equipment. Non-standard initial settings of the equipment (equipment originally intended for human samples, but now applied to animal samples) was the main cause of the wide range of analytical values seen.

Original languageEnglish
Pages (from-to)25-44
Number of pages20
JournalJournal of Toxicological Sciences
Volume22
Issue number1
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Clinical Chemistry
Rats
Blood
Albumins
Aspartate Aminotransferases
Serum
Alanine Transaminase
Bilirubin
Equipment and Supplies
Creatinine
Reference Values
Surveys and Questionnaires
Clinical Pathology
Potassium Chloride
Calcium Chloride
Globulins
Pathology
Toxicology
Electrophoresis
Alkaline Phosphatase

Keywords

  • Clinical chemistry
  • Control survey
  • Rat
  • Variation between Interlaboratory variation

ASJC Scopus subject areas

  • Toxicology

Cite this

Matsuzawa, T., Hayashi, Y., Nomura, M., Unno, T., Igarashi, T., Furuya, T., ... Hayashi, Y. (1997). A survey of the values of clinical chemistry parameters obtained for a common rat blood sample in ninety-eight Japanese laboratories. Journal of Toxicological Sciences, 22(1), 25-44.

A survey of the values of clinical chemistry parameters obtained for a common rat blood sample in ninety-eight Japanese laboratories. / Matsuzawa, Toshiaki; Hayashi, Yutaka; Nomura, Mamoru; Unno, Takashi; Igarashi, Toshiji; Furuya, Tsuyoshi; Sekita, Kiyoshi; Ono, Atsushi; Kurokawa, Yuji; Hayashi, Yuzo.

In: Journal of Toxicological Sciences, Vol. 22, No. 1, 1997, p. 25-44.

Research output: Contribution to journalArticle

Matsuzawa, T, Hayashi, Y, Nomura, M, Unno, T, Igarashi, T, Furuya, T, Sekita, K, Ono, A, Kurokawa, Y & Hayashi, Y 1997, 'A survey of the values of clinical chemistry parameters obtained for a common rat blood sample in ninety-eight Japanese laboratories', Journal of Toxicological Sciences, vol. 22, no. 1, pp. 25-44.
Matsuzawa, Toshiaki ; Hayashi, Yutaka ; Nomura, Mamoru ; Unno, Takashi ; Igarashi, Toshiji ; Furuya, Tsuyoshi ; Sekita, Kiyoshi ; Ono, Atsushi ; Kurokawa, Yuji ; Hayashi, Yuzo. / A survey of the values of clinical chemistry parameters obtained for a common rat blood sample in ninety-eight Japanese laboratories. In: Journal of Toxicological Sciences. 1997 ; Vol. 22, No. 1. pp. 25-44.
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