A study on cross-reactivity of anti-DNA antibody with glycosaminoglycans.

N. Kashihara, S. Hirakawa, Y. Mino, Hirofumi Makino, Z. Ota

Research output: Contribution to journalArticle

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Abstract

To study the pathogenesis of lupus nephritis, the cross reactivity between anti-DNA antibody and glycosaminoglycans (GAGs) was investigated. Monoclonal anti-DNA antibodies were obtained from hybridomas by the fusion of MRL/lpr/lpr splenocytes with murine myeloma cells. Some of these monoclonal anti-DNA antibodies showed cross reactivity with GAGs, such as hyaluronic acid, chondroitin sulfate and heparan sulfate. To elucidate the mechanism of cross reactivity, inhibition assays with propanol and polyethylenimine (PEI), a cationic agent, were carried out. Increase of the concentration of PEI (0.6-2.0% vol/vol) resulted in a dose dependent decrease in the binding ability of anti-DNA antibody to GAGs. Propanol, an organic reagent which disrupts the van der Waals bonds between epitopes and paratopes, showed little inhibitory effect on the binding activity of monoclonal anti-DNA antibody to GAGs. These results indicate that the binding of anti-DNA antibody to GAGs is due to a charge interaction rather than van der Waals forces. Anti-DNA antibody which can react with GAGs in the glomerular basement membrane seems to play an important role in the pathogenesis of lupus nephritis.

Original languageEnglish
Pages (from-to)255-259
Number of pages5
JournalActa Medica Okayama
Volume47
Issue number4
Publication statusPublished - Aug 1 1993

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Antinuclear Antibodies
Glycosaminoglycans
Polyethyleneimine
1-Propanol
Lupus Nephritis
Monoclonal Antibodies
Antibody Binding Sites
Glomerular Basement Membrane
Van der Waals forces
Heparitin Sulfate
Chondroitin Sulfates
Hybridomas
Hyaluronic Acid
Epitopes
Assays
Fusion reactions

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kashihara, N., Hirakawa, S., Mino, Y., Makino, H., & Ota, Z. (1993). A study on cross-reactivity of anti-DNA antibody with glycosaminoglycans. Acta Medica Okayama, 47(4), 255-259.

A study on cross-reactivity of anti-DNA antibody with glycosaminoglycans. / Kashihara, N.; Hirakawa, S.; Mino, Y.; Makino, Hirofumi; Ota, Z.

In: Acta Medica Okayama, Vol. 47, No. 4, 01.08.1993, p. 255-259.

Research output: Contribution to journalArticle

Kashihara, N, Hirakawa, S, Mino, Y, Makino, H & Ota, Z 1993, 'A study on cross-reactivity of anti-DNA antibody with glycosaminoglycans.', Acta Medica Okayama, vol. 47, no. 4, pp. 255-259.
Kashihara N, Hirakawa S, Mino Y, Makino H, Ota Z. A study on cross-reactivity of anti-DNA antibody with glycosaminoglycans. Acta Medica Okayama. 1993 Aug 1;47(4):255-259.
Kashihara, N. ; Hirakawa, S. ; Mino, Y. ; Makino, Hirofumi ; Ota, Z. / A study on cross-reactivity of anti-DNA antibody with glycosaminoglycans. In: Acta Medica Okayama. 1993 ; Vol. 47, No. 4. pp. 255-259.
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