A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus

Kanae Muraiso, Makoto Hijikata, Showgo Ohkoshi, Myung Je Cho, Masayoshi Kikuchi, Nobuyuki Kato, Kunitada Shimotohno

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.

Original languageEnglish
Pages (from-to)511-516
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number2
DOIs
Publication statusPublished - Oct 30 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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