A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus

Kanae Muraiso, Makoto Hijikata, Showgo Ohkoshi, Myung Je Cho, Masayoshi Kikuchi, Nobuyuki Kato, Kunitada Shimotohno

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A putative core protein derived from hepatitis C virus was expressed in E. coli. More than 5% of the total protein expressed in the bacteria after induction by isopropylthio-beta-D-galactoside was shown to be the expected protein. Western blotting with this E. coli lysate proved to be more efficient than ELISA with a non-structural viral protein, C100, to detect infection of hepatitis C virus in the sera of patients with non-A, non-B chronic hepatitis, hepatocellular carcinoma as well as in sera from healthy persons.

Original languageEnglish
Pages (from-to)511-516
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume172
Issue number2
DOIs
Publication statusPublished - Oct 30 1990
Externally publishedYes

Fingerprint

Protein C
Viruses
Hepacivirus
Escherichia coli
Viral Nonstructural Proteins
Chronic Hepatitis
Serum
Galactosides
Hepatocellular Carcinoma
Proteins
Western Blotting
Enzyme-Linked Immunosorbent Assay
Bacteria
Infection
beta-galactoside
Hepatitis C virus nucleocapsid protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus. / Muraiso, Kanae; Hijikata, Makoto; Ohkoshi, Showgo; Cho, Myung Je; Kikuchi, Masayoshi; Kato, Nobuyuki; Shimotohno, Kunitada.

In: Biochemical and Biophysical Research Communications, Vol. 172, No. 2, 30.10.1990, p. 511-516.

Research output: Contribution to journalArticle

Muraiso, Kanae ; Hijikata, Makoto ; Ohkoshi, Showgo ; Cho, Myung Je ; Kikuchi, Masayoshi ; Kato, Nobuyuki ; Shimotohno, Kunitada. / A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 172, No. 2. pp. 511-516.
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