Abstract
A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC50 of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC50 of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.
| Original language | English |
|---|---|
| Pages (from-to) | 738-742 |
| Number of pages | 5 |
| Journal | Zeitschrift fur Naturforschung - Section C Journal of Biosciences |
| Volume | 51 |
| Issue number | 9-10 |
| Publication status | Published - Sep 1996 |
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Keywords
- Chitinase Inhibitory Assay
- Loculoascomycete Metabolites
- Screening
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
Cite this
A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors. / Kawazu, Kazuyoshi; Ohnishi, Shigehiko; Kanzaki, Hiroshi; Kobayashi, Akio.
In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 51, No. 9-10, 09.1996, p. 738-742.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors
AU - Kawazu, Kazuyoshi
AU - Ohnishi, Shigehiko
AU - Kanzaki, Hiroshi
AU - Kobayashi, Akio
PY - 1996/9
Y1 - 1996/9
N2 - A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC50 of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC50 of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.
AB - A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC50 of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC50 of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.
KW - Chitinase Inhibitory Assay
KW - Loculoascomycete Metabolites
KW - Screening
UR - http://www.scopus.com/inward/record.url?scp=0030464813&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030464813&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0030464813
VL - 51
SP - 738
EP - 742
JO - Zeitschrift fur Naturforschung. Section C: Biosciences
JF - Zeitschrift fur Naturforschung. Section C: Biosciences
SN - 0939-5075
IS - 9-10
ER -