A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors

Kazuyoshi Kawazu; Shigehiko Ohnishi; Hiroshi Kanzaki; Akio Kobayashi

doi:10.1515/znc-1996-9-1020

A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors

Kazuyoshi Kawazu, Shigehiko Ohnishi, Hiroshi Kanzaki, Akio Kobayashi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC₅₀ of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC₅₀ of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.

Original language	English
Pages (from-to)	738-742
Number of pages	5
Journal	Zeitschrift fur Naturforschung Section C - Journal of Biosciences
Volume	51
Issue number	9-10
DOIs	https://doi.org/10.1515/znc-1996-9-1020
Publication status	Published - 1996

Keywords

Chitinase Inhibitory Assay
Loculoascomycete Metabolites
Screening

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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title = "A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors",

abstract = "A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC50 of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC50 of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.",

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author = "Kazuyoshi Kawazu and Shigehiko Ohnishi and Hiroshi Kanzaki and Akio Kobayashi",

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AU - Kawazu, Kazuyoshi

AU - Ohnishi, Shigehiko

AU - Kanzaki, Hiroshi

AU - Kobayashi, Akio

N1 - Funding Information: This work was supported partly by the Chemical Materials Research and Development Foun-

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