A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors

Kazuyoshi Kawazu; Shigehiko Ohnishi; Hiroshi Kanzaki; Akio Kobayashi

doi:10.1515/znc-1996-9-1020

A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors

Kazuyoshi Kawazu, Shigehiko Ohnishi, Hiroshi Kanzaki, Akio Kobayashi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC₅₀ of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC₅₀ of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.

Original language	English
Pages (from-to)	738-742
Number of pages	5
Journal	Zeitschrift fur Naturforschung Section C - Journal of Biosciences
Volume	51
Issue number	9-10
DOIs	https://doi.org/10.1515/znc-1996-9-1020
Publication status	Published - 1996

Keywords

Chitinase Inhibitory Assay
Loculoascomycete Metabolites
Screening

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1515/znc-1996-9-1020

Fingerprint Dive into the research topics of 'A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors'. Together they form a unique fingerprint.

Cite this

@article{1187571322ff4120b22b71cc5c3beda4,

title = "A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors",

abstract = "A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC50 of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC50 of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.",

keywords = "Chitinase Inhibitory Assay, Loculoascomycete Metabolites, Screening",

author = "Kazuyoshi Kawazu and Shigehiko Ohnishi and Hiroshi Kanzaki and Akio Kobayashi",

note = "Funding Information: This work was supported partly by the Chemical Materials Research and Development Foun-",

year = "1996",

doi = "10.1515/znc-1996-9-1020",

language = "English",

volume = "51",

pages = "738--742",

journal = "Zeitschrift fur Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie",

issn = "0939-5075",

publisher = "Verlag der Zeitschrift fur Naturforschung",

number = "9-10",

}

TY - JOUR

T1 - A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors

AU - Kawazu, Kazuyoshi

AU - Ohnishi, Shigehiko

AU - Kanzaki, Hiroshi

AU - Kobayashi, Akio

N1 - Funding Information: This work was supported partly by the Chemical Materials Research and Development Foun-

PY - 1996

Y1 - 1996

N2 - A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC50 of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC50 of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.

AB - A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC50 of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC50 of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.

KW - Chitinase Inhibitory Assay

KW - Loculoascomycete Metabolites

KW - Screening

UR - http://www.scopus.com/inward/record.url?scp=0030464813&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030464813&partnerID=8YFLogxK

U2 - 10.1515/znc-1996-9-1020

DO - 10.1515/znc-1996-9-1020

M3 - Article

AN - SCOPUS:0030464813

VL - 51

SP - 738

EP - 742

JO - Zeitschrift fur Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie

JF - Zeitschrift fur Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie

SN - 0939-5075

IS - 9-10

ER -

A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this