TY - JOUR
T1 - A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors
AU - Kawazu, Kazuyoshi
AU - Ohnishi, Shigehiko
AU - Kanzaki, Hiroshi
AU - Kobayashi, Akio
N1 - Funding Information:
This work was supported partly by the Chemical Materials Research and Development Foun-
PY - 1996
Y1 - 1996
N2 - A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC50 of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC50 of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.
AB - A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC50 of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC50 of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.
KW - Chitinase Inhibitory Assay
KW - Loculoascomycete Metabolites
KW - Screening
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U2 - 10.1515/znc-1996-9-1020
DO - 10.1515/znc-1996-9-1020
M3 - Article
AN - SCOPUS:0030464813
VL - 51
SP - 738
EP - 742
JO - Zeitschrift fur Naturforschung. Section C: Biosciences
JF - Zeitschrift fur Naturforschung. Section C: Biosciences
SN - 0939-5075
IS - 9-10
ER -