A stable crude chitinase solution from Spodoptera litura pupae and a search for its inhibitors

Kazuyoshi Kawazu, Shigehiko Ohnishi, Hiroshi Kanzaki, Akio Kobayashi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A convenient method for preparing a stable chitinase solution from a pest insect, Spodoptera litura, and a bioassay method for insect chitinase inhibitors using this enzyme solution were developed. Allosamidin, the first insect chitinase inhibitor, exhibited an IC50 of 0.2 μM in the present Spodoptera litura chitinase assay, whereas allosamidin was reported to exhibit an IC50 of 0.7 μM in the Bombyx mori chitinase assay. Thus, the present assay using Spodoptera litura chitinase was 3.5-fold more sensitive than the B. mori chitinase assay. Screening of 300 supernatants of microbial cultures for chitinase inhibitors using the Spodoptera litura chitinase assay found 13 active supernatants.

Original languageEnglish
Pages (from-to)738-742
Number of pages5
JournalZeitschrift fur Naturforschung Section C - Journal of Biosciences
Volume51
Issue number9-10
DOIs
Publication statusPublished - 1996

Keywords

  • Chitinase Inhibitory Assay
  • Loculoascomycete Metabolites
  • Screening

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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