A spectroscopic variant of the light-harvesting 1 core complex from the thermophilic purple sulfur bacterium thermochromatium tepidum

Yukihiro Kimura, Yuta Inada, Long-Jiang Yu, Zheng Yu Wang, Takashi Ohno

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Thermochromatium tepidum is a purple sulfur photosynthetic bacterium, and its light-harvesting 1 reaction center (LH1RC) complexes exhibit an unusual LH1 Qy absorption at 915 nm (B915) and possess enhanced thermal stability. These unique properties are closely related to an inorganic cofactor, Ca2+. Here, we report a spectroscopic variant of LH1RC complexes from Tch. tepidum cells in which Ca2+ was biosynthetically replaced with Sr2+. The photosynthetic growth of wild-type cells cannot be maintained without Ca2+ and is heavily inhibited when the Ca 2+ is replaced with other metal cations. Interestingly, only Sr 2+ supported photosynthetic growth instead of Ca2+ with slightly reduced rates. The resulting Sr-tepidum cells exhibited characteristic absorption spectra in the LH1 Qy region with different LH1RC:LH2 ratios depending on the growth conditions. LH1RC complexes purified from the Sr-tepidum cells exhibited a Qy maximum at 888 nm (B888) that was blue-shifted after removal of Sr2+ to ∼870 nm (B870). Reconstitution of Sr2+ and Ca2+ into B870 resulted in red shifts of the Qy peak to 888 and 908 nm, respectively. The thermal stability of B888 was slightly lower than that of B915 as revealed by differential scanning calorimetry analysis. Effects of other divalent metal cations on the Qy peak position and thermal stability of B888 were similar but not identical to those of B915. This study provides the first evidence of a purple bacterium in which LH1RC complexes alter spectroscopic and thermodynamic properties in vivo by utilizing exogenous metal cations and improve the ability to adapt to the environmental changes.

Original languageEnglish
Pages (from-to)3638-3648
Number of pages11
JournalBiochemistry
Volume50
Issue number18
DOIs
Publication statusPublished - May 10 2011
Externally publishedYes

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Chromatiaceae
Sulfur
Bacteria
Light
Cations
Thermodynamic stability
Hot Temperature
Metals
Growth
Proteobacteria
Divalent Cations
Differential Scanning Calorimetry
Thermodynamics
Absorption spectra
Differential scanning calorimetry
Thermodynamic properties
Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

A spectroscopic variant of the light-harvesting 1 core complex from the thermophilic purple sulfur bacterium thermochromatium tepidum. / Kimura, Yukihiro; Inada, Yuta; Yu, Long-Jiang; Wang, Zheng Yu; Ohno, Takashi.

In: Biochemistry, Vol. 50, No. 18, 10.05.2011, p. 3638-3648.

Research output: Contribution to journalArticle

Kimura, Yukihiro ; Inada, Yuta ; Yu, Long-Jiang ; Wang, Zheng Yu ; Ohno, Takashi. / A spectroscopic variant of the light-harvesting 1 core complex from the thermophilic purple sulfur bacterium thermochromatium tepidum. In: Biochemistry. 2011 ; Vol. 50, No. 18. pp. 3638-3648.
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