Abstract
Objective: Streptococcus mutans, a major dental caries pathogen, has shown to be associated with the aggravation of cerebral hemorrhage and inflammatory bowel diseases. In this study, we evaluated the effects of S. mutans on the development of non-alcoholic steatohepatitis (NASH) in a mouse model. Materials and methods: Streptococcus mutans oral strain MT8148 (serotype c) and a blood isolate TW871 (k) were used. C57BL/6J mice (6 weeks old) were fed a high-fat diet for 4 weeks; the test strains or phosphate-buffered saline was then intravenously administered. Mice were euthanized after 8 or 12 weeks. Whole body, extirpated liver, and visceral fat weights were determined, and histopathological evaluations of the liver specimens were performed. Results: Mice infected with TW871 showed significantly greater body and liver weights than those administered MT8148 or phosphate-buffered saline. Histopathological analyses revealed prominent infiltration of inflammatory cells and adipocellular deposition in livers extirpated 8 weeks after an infection with TW871; fibrosis was also observed in livers extirpated after 12 weeks. Conclusion: These results suggest that a specific strain of S. mutans could induce NASH.
| Original language | English |
|---|---|
| Pages (from-to) | 700-706 |
| Number of pages | 7 |
| Journal | Oral Diseases |
| Volume | 20 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Oct 1 2014 |
| Externally published | Yes |
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Keywords
- Streptococcus mutans
- Mouse model
- Non-alcoholic fatty liver diseases
ASJC Scopus subject areas
- Dentistry(all)
- Otorhinolaryngology
Cite this
A specific Streptococcus mutans strain aggravates non-alcoholic fatty liver disease. / Naka, Shuhei; Nomura, R.; Takashima, Yukiko; Okawa, R.; Ooshima, T.; Nakano, K.
In: Oral Diseases, Vol. 20, No. 7, 01.10.2014, p. 700-706.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A specific Streptococcus mutans strain aggravates non-alcoholic fatty liver disease
AU - Naka, Shuhei
AU - Nomura, R.
AU - Takashima, Yukiko
AU - Okawa, R.
AU - Ooshima, T.
AU - Nakano, K.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Objective: Streptococcus mutans, a major dental caries pathogen, has shown to be associated with the aggravation of cerebral hemorrhage and inflammatory bowel diseases. In this study, we evaluated the effects of S. mutans on the development of non-alcoholic steatohepatitis (NASH) in a mouse model. Materials and methods: Streptococcus mutans oral strain MT8148 (serotype c) and a blood isolate TW871 (k) were used. C57BL/6J mice (6 weeks old) were fed a high-fat diet for 4 weeks; the test strains or phosphate-buffered saline was then intravenously administered. Mice were euthanized after 8 or 12 weeks. Whole body, extirpated liver, and visceral fat weights were determined, and histopathological evaluations of the liver specimens were performed. Results: Mice infected with TW871 showed significantly greater body and liver weights than those administered MT8148 or phosphate-buffered saline. Histopathological analyses revealed prominent infiltration of inflammatory cells and adipocellular deposition in livers extirpated 8 weeks after an infection with TW871; fibrosis was also observed in livers extirpated after 12 weeks. Conclusion: These results suggest that a specific strain of S. mutans could induce NASH.
AB - Objective: Streptococcus mutans, a major dental caries pathogen, has shown to be associated with the aggravation of cerebral hemorrhage and inflammatory bowel diseases. In this study, we evaluated the effects of S. mutans on the development of non-alcoholic steatohepatitis (NASH) in a mouse model. Materials and methods: Streptococcus mutans oral strain MT8148 (serotype c) and a blood isolate TW871 (k) were used. C57BL/6J mice (6 weeks old) were fed a high-fat diet for 4 weeks; the test strains or phosphate-buffered saline was then intravenously administered. Mice were euthanized after 8 or 12 weeks. Whole body, extirpated liver, and visceral fat weights were determined, and histopathological evaluations of the liver specimens were performed. Results: Mice infected with TW871 showed significantly greater body and liver weights than those administered MT8148 or phosphate-buffered saline. Histopathological analyses revealed prominent infiltration of inflammatory cells and adipocellular deposition in livers extirpated 8 weeks after an infection with TW871; fibrosis was also observed in livers extirpated after 12 weeks. Conclusion: These results suggest that a specific strain of S. mutans could induce NASH.
KW - Streptococcus mutans
KW - Mouse model
KW - Non-alcoholic fatty liver diseases
UR - http://www.scopus.com/inward/record.url?scp=84908373046&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908373046&partnerID=8YFLogxK
U2 - 10.1111/odi.12191
DO - 10.1111/odi.12191
M3 - Article
C2 - 25360469
AN - SCOPUS:84908373046
VL - 20
SP - 700
EP - 706
JO - Oral Diseases
JF - Oral Diseases
SN - 1354-523X
IS - 7
ER -