TY - JOUR
T1 - A solvent effect on the mutagenicity of tryptophan-pyrolysate mutagens in the Salmonella/mammalian microsome assay
AU - Arimoto, Sakae
AU - Nakano, Narumi
AU - Ohara, Yoshiko
AU - Tanaka, Kazumi
AU - Hayatsu, Hikoya
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Cancer Research from the Ministry of Health and Welfare, a Grant for Environmental Research from the Ministry of Education, Culture and Science, and a grant from the Naito Science Foundation.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1982/9
Y1 - 1982/9
N2 - The effect of adding organic solvents to the pre-incubation mixture in the Salmonella/mammalian microsome assay was examined in tests of the tryptophanpyrolysate mutagens, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2). Acetonitrile enhanced the mutagenic activity of Trp-P-1 and Trp-P-2 by an order of magnitude under optimal conditions. Strong enhancement of mutagenicity was also observed for the addition of ethanol, methanol, tetrahydrofurfuryl alcohol, tetrahydrofuran, 1,2-dimethoxyethane and N,N-dimethylformamide. The enhancing effect of acetonitrile on the mutagenicity of Trp-P-2 was found to be result of enrichment of the active metabolite in the assay system.
AB - The effect of adding organic solvents to the pre-incubation mixture in the Salmonella/mammalian microsome assay was examined in tests of the tryptophanpyrolysate mutagens, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2). Acetonitrile enhanced the mutagenic activity of Trp-P-1 and Trp-P-2 by an order of magnitude under optimal conditions. Strong enhancement of mutagenicity was also observed for the addition of ethanol, methanol, tetrahydrofurfuryl alcohol, tetrahydrofuran, 1,2-dimethoxyethane and N,N-dimethylformamide. The enhancing effect of acetonitrile on the mutagenicity of Trp-P-2 was found to be result of enrichment of the active metabolite in the assay system.
UR - http://www.scopus.com/inward/record.url?scp=0019975081&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019975081&partnerID=8YFLogxK
U2 - 10.1016/0165-1218(82)90111-2
DO - 10.1016/0165-1218(82)90111-2
M3 - Article
C2 - 6755228
AN - SCOPUS:0019975081
VL - 102
SP - 105
EP - 112
JO - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
JF - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
SN - 0165-1218
IS - 2
ER -