Abstract
The effect of adding organic solvents to the pre-incubation mixture in the Salmonella/mammalian microsome assay was examined in tests of the tryptophanpyrolysate mutagens, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2). Acetonitrile enhanced the mutagenic activity of Trp-P-1 and Trp-P-2 by an order of magnitude under optimal conditions. Strong enhancement of mutagenicity was also observed for the addition of ethanol, methanol, tetrahydrofurfuryl alcohol, tetrahydrofuran, 1,2-dimethoxyethane and N,N-dimethylformamide. The enhancing effect of acetonitrile on the mutagenicity of Trp-P-2 was found to be result of enrichment of the active metabolite in the assay system.
| Original language | English |
|---|---|
| Pages (from-to) | 105-112 |
| Number of pages | 8 |
| Journal | Mutation Research/Genetic Toxicology |
| Volume | 102 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1982 |
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ASJC Scopus subject areas
- Genetics
- Toxicology
- Medicine(all)
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A solvent effect on the mutagenicity of tryptophan-pyrolysate mutagens in the Salmonella/mammalian microsome assay. / Arimoto, Sakae; Nakano, Narumi; Ohara, Yoshiko; Tanaka, Kazumi; Hayatsu, Hikoya.
In: Mutation Research/Genetic Toxicology, Vol. 102, No. 2, 1982, p. 105-112.Research output: Contribution to journal › Article
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TY - JOUR
T1 - A solvent effect on the mutagenicity of tryptophan-pyrolysate mutagens in the Salmonella/mammalian microsome assay
AU - Arimoto, Sakae
AU - Nakano, Narumi
AU - Ohara, Yoshiko
AU - Tanaka, Kazumi
AU - Hayatsu, Hikoya
PY - 1982
Y1 - 1982
N2 - The effect of adding organic solvents to the pre-incubation mixture in the Salmonella/mammalian microsome assay was examined in tests of the tryptophanpyrolysate mutagens, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2). Acetonitrile enhanced the mutagenic activity of Trp-P-1 and Trp-P-2 by an order of magnitude under optimal conditions. Strong enhancement of mutagenicity was also observed for the addition of ethanol, methanol, tetrahydrofurfuryl alcohol, tetrahydrofuran, 1,2-dimethoxyethane and N,N-dimethylformamide. The enhancing effect of acetonitrile on the mutagenicity of Trp-P-2 was found to be result of enrichment of the active metabolite in the assay system.
AB - The effect of adding organic solvents to the pre-incubation mixture in the Salmonella/mammalian microsome assay was examined in tests of the tryptophanpyrolysate mutagens, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2). Acetonitrile enhanced the mutagenic activity of Trp-P-1 and Trp-P-2 by an order of magnitude under optimal conditions. Strong enhancement of mutagenicity was also observed for the addition of ethanol, methanol, tetrahydrofurfuryl alcohol, tetrahydrofuran, 1,2-dimethoxyethane and N,N-dimethylformamide. The enhancing effect of acetonitrile on the mutagenicity of Trp-P-2 was found to be result of enrichment of the active metabolite in the assay system.
UR - http://www.scopus.com/inward/record.url?scp=0019975081&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019975081&partnerID=8YFLogxK
U2 - 10.1016/0165-1218(82)90111-2
DO - 10.1016/0165-1218(82)90111-2
M3 - Article
VL - 102
SP - 105
EP - 112
JO - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
JF - Mutation Research - Genetic Toxicology Testing and Biomonitoring of Environmental or Occupational Exposure
SN - 0165-1218
IS - 2
ER -