A solvent effect on the mutagenicity of tryptophan-pyrolysate mutagens in the Salmonella/mammalian microsome assay

Sakae Arimoto, Narumi Nakano, Yoshiko Ohara, Kazumi Tanaka, Hikoya Hayatsu

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The effect of adding organic solvents to the pre-incubation mixture in the Salmonella/mammalian microsome assay was examined in tests of the tryptophanpyrolysate mutagens, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2). Acetonitrile enhanced the mutagenic activity of Trp-P-1 and Trp-P-2 by an order of magnitude under optimal conditions. Strong enhancement of mutagenicity was also observed for the addition of ethanol, methanol, tetrahydrofurfuryl alcohol, tetrahydrofuran, 1,2-dimethoxyethane and N,N-dimethylformamide. The enhancing effect of acetonitrile on the mutagenicity of Trp-P-2 was found to be result of enrichment of the active metabolite in the assay system.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalMutation Research/Genetic Toxicology
Volume102
Issue number2
DOIs
Publication statusPublished - 1982

Fingerprint

Salmonella
Mutagens
Microsomes
Assays
Dimethylformamide
Metabolites
Organic solvents
Methanol
Ethanol
3-amino-1-methyl-5H-pyrido(4,3-b)indole
3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole
acetonitrile

ASJC Scopus subject areas

  • Genetics
  • Toxicology
  • Medicine(all)

Cite this

A solvent effect on the mutagenicity of tryptophan-pyrolysate mutagens in the Salmonella/mammalian microsome assay. / Arimoto, Sakae; Nakano, Narumi; Ohara, Yoshiko; Tanaka, Kazumi; Hayatsu, Hikoya.

In: Mutation Research/Genetic Toxicology, Vol. 102, No. 2, 1982, p. 105-112.

Research output: Contribution to journalArticle

Arimoto, Sakae ; Nakano, Narumi ; Ohara, Yoshiko ; Tanaka, Kazumi ; Hayatsu, Hikoya. / A solvent effect on the mutagenicity of tryptophan-pyrolysate mutagens in the Salmonella/mammalian microsome assay. In: Mutation Research/Genetic Toxicology. 1982 ; Vol. 102, No. 2. pp. 105-112.
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