A solvent effect on the mutagenicity of tryptophan-pyrolysate mutagens in the Salmonella/mammalian microsome assay

Sakae Arimoto, Narumi Nakano, Yoshiko Ohara, Kazumi Tanaka, Hikoya Hayatsu

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Abstract

The effect of adding organic solvents to the pre-incubation mixture in the Salmonella/mammalian microsome assay was examined in tests of the tryptophanpyrolysate mutagens, 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2). Acetonitrile enhanced the mutagenic activity of Trp-P-1 and Trp-P-2 by an order of magnitude under optimal conditions. Strong enhancement of mutagenicity was also observed for the addition of ethanol, methanol, tetrahydrofurfuryl alcohol, tetrahydrofuran, 1,2-dimethoxyethane and N,N-dimethylformamide. The enhancing effect of acetonitrile on the mutagenicity of Trp-P-2 was found to be result of enrichment of the active metabolite in the assay system.

Original languageEnglish
Pages (from-to)105-112
Number of pages8
JournalMutation Research/Genetic Toxicology
Volume102
Issue number2
DOIs
Publication statusPublished - Sep 1982

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ASJC Scopus subject areas

  • Toxicology
  • Genetics

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