A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu, Zn-superoxide dismutase

Shinya Hanashima, Noriko Fujiwara, Kana Matsumoto, Noriyuki Iwasaki, Guo Qing Zheng, Hidetaka Torigoe, Keiichiro Suzuki, Naoyuki Taniguchibgh, Yoshiki Yamaguchi

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4 Citations (Scopus)

Abstract

Solution 17O-NMR application to biological macromolecules is extremely limited. We describe here 17O-NMR observation of the 17O2-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective 17O2 oxidation. 17O-NMR with the aid of 17O-labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.

Original languageEnglish
Pages (from-to)1449-1451
Number of pages3
JournalChemical Communications
Volume49
Issue number14
DOIs
Publication statusPublished - Jan 22 2013

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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    Hanashima, S., Fujiwara, N., Matsumoto, K., Iwasaki, N., Zheng, G. Q., Torigoe, H., Suzuki, K., Taniguchibgh, N., & Yamaguchi, Y. (2013). A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu, Zn-superoxide dismutase. Chemical Communications, 49(14), 1449-1451. https://doi.org/10.1039/c2cc36918d