A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu, Zn-superoxide dismutase

Shinya Hanashima; Noriko Fujiwara; Kana Matsumoto; Noriyuki Iwasaki; Guo Qing Zheng; Hidetaka Torigoe; Keiichiro Suzuki; Naoyuki Taniguchibgh; Yoshiki Yamaguchi

doi:10.1039/c2cc36918d

A solution ¹⁷O-NMR approach for observing an oxidized cysteine residue in Cu, Zn-superoxide dismutase

Shinya Hanashima, Noriko Fujiwara, Kana Matsumoto, Noriyuki Iwasaki, Guo Qing Zheng, Hidetaka Torigoe, Keiichiro Suzuki, Naoyuki Taniguchibgh, Yoshiki Yamaguchi

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Solution ¹⁷O-NMR application to biological macromolecules is extremely limited. We describe here ¹⁷O-NMR observation of the ¹⁷O₂-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective ¹⁷O₂ oxidation. ¹⁷O-NMR with the aid of ¹⁷O-labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.

Original language	English
Pages (from-to)	1449-1451
Number of pages	3
Journal	Chemical Communications
Volume	49
Issue number	14
DOIs	https://doi.org/10.1039/c2cc36918d
Publication status	Published - Jan 22 2013

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/c2cc36918d

Cite this

A solution ¹⁷O-NMR approach for observing an oxidized cysteine residue in Cu, Zn-superoxide dismutase. / Hanashima, Shinya; Fujiwara, Noriko; Matsumoto, Kana; Iwasaki, Noriyuki; Zheng, Guo Qing; Torigoe, Hidetaka; Suzuki, Keiichiro; Taniguchibgh, Naoyuki; Yamaguchi, Yoshiki.

In: Chemical Communications, Vol. 49, No. 14, 22.01.2013, p. 1449-1451.

Research output: Contribution to journal › Article › peer-review

@article{d95fa428610d42aabe4ddb41fab0c000,

title = "A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu, Zn-superoxide dismutase",

abstract = "Solution 17O-NMR application to biological macromolecules is extremely limited. We describe here 17O-NMR observation of the 17O2-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective 17O2 oxidation. 17O-NMR with the aid of 17O-labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.",

author = "Shinya Hanashima and Noriko Fujiwara and Kana Matsumoto and Noriyuki Iwasaki and Zheng, {Guo Qing} and Hidetaka Torigoe and Keiichiro Suzuki and Naoyuki Taniguchibgh and Yoshiki Yamaguchi",

year = "2013",

month = jan,

day = "22",

doi = "10.1039/c2cc36918d",

language = "English",

volume = "49",

pages = "1449--1451",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "14",

}

TY - JOUR

T1 - A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu, Zn-superoxide dismutase

AU - Hanashima, Shinya

AU - Fujiwara, Noriko

AU - Matsumoto, Kana

AU - Iwasaki, Noriyuki

AU - Zheng, Guo Qing

AU - Torigoe, Hidetaka

AU - Suzuki, Keiichiro

AU - Taniguchibgh, Naoyuki

AU - Yamaguchi, Yoshiki

PY - 2013/1/22

Y1 - 2013/1/22

N2 - Solution 17O-NMR application to biological macromolecules is extremely limited. We describe here 17O-NMR observation of the 17O2-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective 17O2 oxidation. 17O-NMR with the aid of 17O-labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.

AB - Solution 17O-NMR application to biological macromolecules is extremely limited. We describe here 17O-NMR observation of the 17O2-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective 17O2 oxidation. 17O-NMR with the aid of 17O-labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.

UR - http://www.scopus.com/inward/record.url?scp=84872717758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872717758&partnerID=8YFLogxK

U2 - 10.1039/c2cc36918d

DO - 10.1039/c2cc36918d

M3 - Article

C2 - 23323266

AN - SCOPUS:84872717758

VL - 49

SP - 1449

EP - 1451

JO - Chemical Communications

JF - Chemical Communications

SN - 1359-7345

IS - 14

ER -

A solution ¹⁷O-NMR approach for observing an oxidized cysteine residue in Cu, Zn-superoxide dismutase

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this