A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase

Shinya Hanashima, Noriko Fujiwara, Kana Matsumoto, Noriyuki Iwasaki, Guo-Qing Zheng, Hidetaka Torigoe, Keiichiro Suzuki, Naoyuki Taniguchi, Yoshiki Yamaguchi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Solution 17O-NMR application to biological macromolecules is extremely limited. We describe here 17O-NMR observation of the 17O2-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective 17O 2 oxidation. 17O-NMR with the aid of 17O- labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.

Original languageEnglish
Pages (from-to)1449-1451
Number of pages3
JournalChemical Communications
Volume49
Issue number14
DOIs
Publication statusPublished - Feb 18 2013

    Fingerprint

ASJC Scopus subject areas

  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Catalysis
  • Chemistry(all)
  • Medicine(all)

Cite this

Hanashima, S., Fujiwara, N., Matsumoto, K., Iwasaki, N., Zheng, G-Q., Torigoe, H., Suzuki, K., Taniguchi, N., & Yamaguchi, Y. (2013). A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase. Chemical Communications, 49(14), 1449-1451. https://doi.org/10.1039/c2cc36918d