A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase

Shinya Hanashima, Noriko Fujiwara, Kana Matsumoto, Noriyuki Iwasaki, Guo-Qing Zheng, Hidetaka Torigoe, Keiichiro Suzuki, Naoyuki Taniguchi, Yoshiki Yamaguchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Solution 17O-NMR application to biological macromolecules is extremely limited. We describe here 17O-NMR observation of the 17O2-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective 17O 2 oxidation. 17O-NMR with the aid of 17O- labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.

Original languageEnglish
Pages (from-to)1449-1451
Number of pages3
JournalChemical Communications
Volume49
Issue number14
DOIs
Publication statusPublished - Feb 18 2013

Fingerprint

Cysteine
Nuclear magnetic resonance
Observation
Macromolecules
Labeling
Proteins
Oxidation
Superoxide Dismutase
Superoxide Dismutase-1

ASJC Scopus subject areas

  • Metals and Alloys
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Catalysis
  • Chemistry(all)
  • Medicine(all)

Cite this

Hanashima, S., Fujiwara, N., Matsumoto, K., Iwasaki, N., Zheng, G-Q., Torigoe, H., ... Yamaguchi, Y. (2013). A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase. Chemical Communications, 49(14), 1449-1451. https://doi.org/10.1039/c2cc36918d

A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase. / Hanashima, Shinya; Fujiwara, Noriko; Matsumoto, Kana; Iwasaki, Noriyuki; Zheng, Guo-Qing; Torigoe, Hidetaka; Suzuki, Keiichiro; Taniguchi, Naoyuki; Yamaguchi, Yoshiki.

In: Chemical Communications, Vol. 49, No. 14, 18.02.2013, p. 1449-1451.

Research output: Contribution to journalArticle

Hanashima, S, Fujiwara, N, Matsumoto, K, Iwasaki, N, Zheng, G-Q, Torigoe, H, Suzuki, K, Taniguchi, N & Yamaguchi, Y 2013, 'A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase', Chemical Communications, vol. 49, no. 14, pp. 1449-1451. https://doi.org/10.1039/c2cc36918d
Hanashima, Shinya ; Fujiwara, Noriko ; Matsumoto, Kana ; Iwasaki, Noriyuki ; Zheng, Guo-Qing ; Torigoe, Hidetaka ; Suzuki, Keiichiro ; Taniguchi, Naoyuki ; Yamaguchi, Yoshiki. / A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase. In: Chemical Communications. 2013 ; Vol. 49, No. 14. pp. 1449-1451.
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