TY - JOUR
T1 - A single-cell atlas of non-haematopoietic cells in human lymph nodes and lymphoma reveals a landscape of stromal remodelling
AU - Abe, Yoshiaki
AU - Sakata-Yanagimoto, Mamiko
AU - Fujisawa, Manabu
AU - Miyoshi, Hiroaki
AU - Suehara, Yasuhito
AU - Hattori, Keiichiro
AU - Kusakabe, Manabu
AU - Sakamoto, Tatsuhiro
AU - Nishikii, Hidekazu
AU - Nguyen, Tran B.
AU - Owada, Yohei
AU - Enomoto, Tsuyoshi
AU - Sawa, Aya
AU - Bando, Hiroko
AU - Yoshida, Chikashi
AU - Tabata, Rikako
AU - Terao, Toshiki
AU - Nakayama, Masahiro
AU - Ohshima, Koichi
AU - Usuki, Kensuke
AU - Oda, Tatsuya
AU - Matsue, Kosei
AU - Chiba, Shigeru
N1 - Funding Information:
We thank our staff at associated institutions and departments for cooperation in collections of the human samples; E. Matsuzawa and Y. Sakashita for technical assistance; and A. Suzuki and Y. Suzuki (the University of Tokyo) for their support in the computational analysis of scRNA-seq data. Finally, we acknowledge E. Lamar for outstanding editorial assistance. This work was supported by Grants-in-Aid for Scientific Research (KAKENHI: JP20J20851 to Y.A., JP21H02945 to M.S.-Y., and JP19H03683 to S.C.) from the Ministry of Education, Culture, Sports, and Science of Japan; AMED under grant numbers JP21ck0106544 and JP21ck0106644 (to M.S.-Y.) and JP21cm0106505 (to S.C.); and Okinaka Memorial Institute for Medical Research, Foundation for Promotion of Cancer Research, and Takeda Science Foundation (to M.S.-Y.). The funders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.
Funding Information:
We thank our staff at associated institutions and departments for cooperation in collections of the human samples; E. Matsuzawa and Y. Sakashita for technical assistance; and A. Suzuki and Y. Suzuki (the University of Tokyo) for their support in the computational analysis of scRNA-seq data. Finally, we acknowledge E. Lamar for outstanding editorial assistance. This work was supported by Grants-in-Aid for Scientific Research (KAKENHI: JP20J20851 to Y.A., JP21H02945 to M.S.-Y., and JP19H03683 to S.C.) from the Ministry of Education, Culture, Sports, and Science of Japan; AMED under grant numbers JP21ck0106544 and JP21ck0106644 (to M.S.-Y.) and JP21cm0106505 (to S.C.); and Okinaka Memorial Institute for Medical Research, Foundation for Promotion of Cancer Research, and Takeda Science Foundation (to M.S.-Y.). The funders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/4
Y1 - 2022/4
N2 - The activities of non-haematopoietic cells (NHCs), including mesenchymal stromal cells and endothelial cells, in lymphomas are reported to underlie lymphomagenesis. However, our understanding of lymphoma NHCs has been hampered by unexplained NHC heterogeneity, even in normal human lymph nodes (LNs). Here we constructed a single-cell transcriptome atlas of more than 100,000 NHCs collected from 27 human samples, including LNs and various nodal lymphomas, and it revealed 30 distinct subclusters, including some that were previously unrecognized. Notably, this atlas was useful for comparative analyses with lymphoma NHCs, which revealed an unanticipated landscape of subcluster-specific changes in gene expression and interaction with malignant cells in follicular lymphoma NHCs. This facilitates our understanding of stromal remodelling in lymphoma and highlights potential clinical biomarkers. Our study largely updates NHC taxonomy in human LNs and analysis of disease status, and provides a rich resource and deeper insights into LN and lymphoma biology to advance lymphoma management and therapy.
AB - The activities of non-haematopoietic cells (NHCs), including mesenchymal stromal cells and endothelial cells, in lymphomas are reported to underlie lymphomagenesis. However, our understanding of lymphoma NHCs has been hampered by unexplained NHC heterogeneity, even in normal human lymph nodes (LNs). Here we constructed a single-cell transcriptome atlas of more than 100,000 NHCs collected from 27 human samples, including LNs and various nodal lymphomas, and it revealed 30 distinct subclusters, including some that were previously unrecognized. Notably, this atlas was useful for comparative analyses with lymphoma NHCs, which revealed an unanticipated landscape of subcluster-specific changes in gene expression and interaction with malignant cells in follicular lymphoma NHCs. This facilitates our understanding of stromal remodelling in lymphoma and highlights potential clinical biomarkers. Our study largely updates NHC taxonomy in human LNs and analysis of disease status, and provides a rich resource and deeper insights into LN and lymphoma biology to advance lymphoma management and therapy.
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UR - http://www.scopus.com/inward/citedby.url?scp=85127470439&partnerID=8YFLogxK
U2 - 10.1038/s41556-022-00866-3
DO - 10.1038/s41556-022-00866-3
M3 - Article
C2 - 35332263
AN - SCOPUS:85127470439
SN - 1465-7392
VL - 24
SP - 565
EP - 578
JO - Nature Cell Biology
JF - Nature Cell Biology
IS - 4
ER -
