A single application of hydrogen sulphide induces a transient osteoclast differentiation with RANKL expression in the rat model

Koichiro Irie, Daisuke Ekuni, Tatsuo Yamamoto, Manabu Morita, Ken Yaegaki, Hisataka Ii, Toshio Imai

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objective: Oral malodor is mainly attributed to volatile sulphur compounds (VSCs) such as hydrogen sulphide (H2S), methyl mercaptan and dimethyl sulphide. VSC accelerate periodontal soft tissue destruction. However, there is little information about the potential role of H2S in alveolar bone loss. The purpose of this animal study was to examine the effects of sodium hydrogen sulphide (NaHS), H2S donor drug, on osteoclast differentiation in rat periodontal tissue. Design: Twenty-four male Wistar rats (8 weeks old) were divided into four groups: a control group and three experimental groups, which were examined at 3 h, 1 day, and 3 days after topical application of 3 μl NaHS (l M in physiological saline) into the gingival sulcus of rat first molar. Expression of tumour necrosis factor (TNF)-α, RANKL, NF-κB and tartrate-resistant acid phosphatase (TRAP) was evaluated in the periodontal tissue. Results: Three hours after NaHS application, TNF-α expression increased in the periodontal ligament. The numbers of RANKL-positive osteoblasts and TRAP-positive osteoclasts significantly increased progressively with time and reached a maximum level after 1 day. Significant up-regulation of RANKL and NF-κB mRNA was observed at 3 h after NaHS application. Conclusions: H2S application caused a transient increase of osteoclast differentiation with up-regulation of RANKL expression in osteoblasts. H2S, which is primarily responsible for halitosis, may also contribute to alveolar bone resorption through RANKL expression.

Original languageEnglish
Pages (from-to)723-729
Number of pages7
JournalArchives of Oral Biology
Volume54
Issue number8
DOIs
Publication statusPublished - Aug 2009

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Hydrogen Sulfide
Osteoclasts
Alveolar Bone Loss
Sulfur Compounds
Osteoblasts
Up-Regulation
Tumor Necrosis Factor-alpha
Halitosis
Periodontal Ligament
Bone Resorption
Sulfhydryl Compounds
Wistar Rats
Control Groups
Messenger RNA
sodium bisulfide
Pharmaceutical Preparations
Tartrate-Resistant Acid Phosphatase

Keywords

  • Hydrogen sulphide
  • Osteoclast
  • Periodontal disease
  • RANKL

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

A single application of hydrogen sulphide induces a transient osteoclast differentiation with RANKL expression in the rat model. / Irie, Koichiro; Ekuni, Daisuke; Yamamoto, Tatsuo; Morita, Manabu; Yaegaki, Ken; Ii, Hisataka; Imai, Toshio.

In: Archives of Oral Biology, Vol. 54, No. 8, 08.2009, p. 723-729.

Research output: Contribution to journalArticle

Irie, Koichiro ; Ekuni, Daisuke ; Yamamoto, Tatsuo ; Morita, Manabu ; Yaegaki, Ken ; Ii, Hisataka ; Imai, Toshio. / A single application of hydrogen sulphide induces a transient osteoclast differentiation with RANKL expression in the rat model. In: Archives of Oral Biology. 2009 ; Vol. 54, No. 8. pp. 723-729.
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