A simplified, sensitive immunohistochemical detection system employing signal amplification based on fluorescyl-tyramide/antifluorescein antibody reaction: Its application to pathologic testing and research

Yutaka Hatanaka, Yuki Imaoka, Kae Torisu, Yuki Kamihara, Kaoru Hashizume, Koichi Ichimura, Tadashi Yoshino, Yoichi Tani

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The catalyzed signal amplification (CSA) technique, based on the peroxidase-mediated deposition of haptenized tyramide and also known as tyramide signal amplification and catalyzed reportor deposition systems, is widely accepted as a signal amplification method for immunohistochemistry and in situ hybridization. In this study, we examined the applicability of a new simplified CSA system employing fluorescyl-tyramide (FT) to pathologic testing and research with formalin-fixed, paraffin-embedded tissues. By using the FT, instead of biotinyl-tyramide (BT) that is commonly employed in the CSA system with chromogen, nonspecific staining caused by endogenous biotin was completely avoided. The FT-CSA system loaded on the automated immunostaining equipment also allowed for more reproducible detection in short times. When applied to cyclin D1 immunostaining that is important in differentiation among small B-cell lymphomas, the system was useful in demonstrating its protein expression in mantle cell lymphomas considered negative or equivocally positive for cyclin D1 in a conventional immunodetection. In immunohistochemistry for phosphorylated proteins and murine hematologic markers that often require higher sensitivity than conventional methods, the FT-CSA system provided desirable staining results with intense signal amplification. Our results indicate that the simplified CSA system employing the FT can be useful in enlarging the target range for routine immunohistochemistry due to its high applicability.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalApplied Immunohistochemistry and Molecular Morphology
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 2008

Fingerprint

Immunohistochemistry
Cyclin D1
Antibodies
Research
Staining and Labeling
Mantle-Cell Lymphoma
B-Cell Lymphoma
B-Cell Chronic Lymphocytic Leukemia
Biotin
Paraffin
Formaldehyde
Peroxidase
In Situ Hybridization
Proteins
Equipment and Supplies

Keywords

  • Fluorescyl-tyramide
  • Tyramide signal amplification

ASJC Scopus subject areas

  • Anatomy
  • Medical Laboratory Technology

Cite this

A simplified, sensitive immunohistochemical detection system employing signal amplification based on fluorescyl-tyramide/antifluorescein antibody reaction : Its application to pathologic testing and research. / Hatanaka, Yutaka; Imaoka, Yuki; Torisu, Kae; Kamihara, Yuki; Hashizume, Kaoru; Ichimura, Koichi; Yoshino, Tadashi; Tani, Yoichi.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 16, No. 1, 01.2008, p. 87-93.

Research output: Contribution to journalArticle

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