A simple method for ion channel recordings using fine gold electrode

Daichi Okuno, Minako Hirano, Hiroaki Yokota, Yukiko Onishi, Junya Ichinose, Toru Ide

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The artificial bilayer single-channel recording technique is commonly used to observe detailed pharmacological properties of various ion channel proteins. It permits easy control of the solution and membrane lipid composition, and is also compatible with pharmacological screening devices. However, its use is limited due to low measurement efficiency. Here, we develop a novel artificial bilayer single-channel recording technique in which bilayers are made and channels are reconstituted into the membranes by contacting a gold electrode to the lipid-solution interface. Using this technique, we measured the single-channel currents of two channel-forming peptides, gramicidin and alamethicin, and a channelforming protein, α-hemolysin. This technique requires only one action, allowing the technique to potentially be combined with high-throughput screening devices.

Original languageEnglish
Pages (from-to)1353-1357
Number of pages5
JournalAnalytical Sciences
Volume32
Issue number12
Publication statusPublished - 2016

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Keywords

  • Artificial bilayer
  • Ion channel
  • Single-channel recording

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Okuno, D., Hirano, M., Yokota, H., Onishi, Y., Ichinose, J., & Ide, T. (2016). A simple method for ion channel recordings using fine gold electrode. Analytical Sciences, 32(12), 1353-1357.