A simple and sensitive radioimmunoassay for adenosine

Ryota Yamane, Takeshi Nakamura, Eiji Matsuura, Hideyuki Ishige, Immunology Laboratory, Diagnostics Division, Masao Fujimoto

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We developed a simple and sensitive radioimmunoassay (RIA) for adenosine. The RIA is based on the double antibody method with adenosine 2',3'-O-disuccinyl-3-[125I]-iodotyrosine methyl ester (diSc-adenosine-[123I]-TME) as a tracer. Anti-adenosine antiserum for the RIA was raised in rabbits immunized with diSc-adenosine conjugated to human serum albumin (diSc-adenosine-HSA). All samples and standards were succinylated prior to assay. The present immunoassay allows detection of 6.25-400 pmol/ml of adenosine in sample. Values obtained by the RIA and by a HPLC analysis showed a high correlation with correlation coefficient of 0.997. In order to determine adenosine in plasmas, blood cells must be separated in the presence of 6 mM EDTA, 0.006% dipyridamole (Dip) and 23 μM 2’-deoxycoformycin (dCF) at 2°C. Adenosine in plasma could be accurately determined by the proposed method even without any pretreatments by deproteinizing. The adenosine levels with or without EDTA-treated normal human plasmas determined were 26.2 ± 7.26 and 100 ± 3.62 pmol/ml (Mean ± SEM), respectively. (KEY WORDS: adenosine, radioimmunoassay (RIA), dipyridamole, plasma adenosine level, EDTA, deoxycoformycin).

Original languageEnglish
Pages (from-to)501-519
Number of pages19
JournalJournal of Immunoassay
Volume12
Issue number4
DOIs
Publication statusPublished - Dec 1 1991
Externally publishedYes

Fingerprint

Adenosine
Radioimmunoassay
Pentostatin
Edetic Acid
Dipyridamole
Plasmas
Plasma (human)
Plasma Cells
Immunoassay
Serum Albumin
Immune Sera
Blood Cells
Assays
High Pressure Liquid Chromatography
Blood
Rabbits
Cells
Antibodies
Scanning electron microscopy

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Yamane, R., Nakamura, T., Matsuura, E., Ishige, H., Laboratory, I., Division, D., & Fujimoto, M. (1991). A simple and sensitive radioimmunoassay for adenosine. Journal of Immunoassay, 12(4), 501-519. https://doi.org/10.1080/01971529108053277

A simple and sensitive radioimmunoassay for adenosine. / Yamane, Ryota; Nakamura, Takeshi; Matsuura, Eiji; Ishige, Hideyuki; Laboratory, Immunology; Division, Diagnostics; Fujimoto, Masao.

In: Journal of Immunoassay, Vol. 12, No. 4, 01.12.1991, p. 501-519.

Research output: Contribution to journalArticle

Yamane, R, Nakamura, T, Matsuura, E, Ishige, H, Laboratory, I, Division, D & Fujimoto, M 1991, 'A simple and sensitive radioimmunoassay for adenosine', Journal of Immunoassay, vol. 12, no. 4, pp. 501-519. https://doi.org/10.1080/01971529108053277
Yamane R, Nakamura T, Matsuura E, Ishige H, Laboratory I, Division D et al. A simple and sensitive radioimmunoassay for adenosine. Journal of Immunoassay. 1991 Dec 1;12(4):501-519. https://doi.org/10.1080/01971529108053277
Yamane, Ryota ; Nakamura, Takeshi ; Matsuura, Eiji ; Ishige, Hideyuki ; Laboratory, Immunology ; Division, Diagnostics ; Fujimoto, Masao. / A simple and sensitive radioimmunoassay for adenosine. In: Journal of Immunoassay. 1991 ; Vol. 12, No. 4. pp. 501-519.
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