A simple and rapid method for analyzing the Monascus pigment-mediated degradation of mutagenic 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole by in-capillary micellar electrokinetic chromatography

Toshiro Watanabe, Tapan Kumar Mazumder, Akira Yamamoto, Shiro Nagai, Sakae Arimoto, Hikoya Hayatsu, Shigeru Terabe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A simple and rapid method is described for analyzing the Monascus pigment-mediated degradation of 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2(NHOH)). We used the in-capillary micellar electrokinetic chromatography (MEKC). During the electrophoresis, the mutagen and the pigment, due to their different migration velocities, mix for a certain period of time to interact, and then they are separated and quantified. Using this technique, we were able to demonstrate that Trp-P-2(NHOH) is degraded by the pigment. The degradation was pigment-dose dependent, and because the pigment was recovered unchanged, it was deduced that the pigment acted catalytically for the degradation. The entire MEKC procedure takes 8 min. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)75-83
Number of pages9
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume444
Issue number1
DOIs
Publication statusPublished - Jul 21 1999

Fingerprint

Monascus
Micellar Electrokinetic Capillary Chromatography
Mutagens
Electrophoresis
Chromatography
3-hydroxyamino-1-methyl-5H-pyrido(4,3-b)indole
3-amino-1-methyl-5H-pyrido(4,3-b)indole

Keywords

  • Antimutagenicity
  • Degradation of mutagen
  • Heterocyclic amine
  • Micellar electrokinetic chromatography
  • Monascus

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Genetics

Cite this

A simple and rapid method for analyzing the Monascus pigment-mediated degradation of mutagenic 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole by in-capillary micellar electrokinetic chromatography. / Watanabe, Toshiro; Mazumder, Tapan Kumar; Yamamoto, Akira; Nagai, Shiro; Arimoto, Sakae; Hayatsu, Hikoya; Terabe, Shigeru.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 444, No. 1, 21.07.1999, p. 75-83.

Research output: Contribution to journalArticle

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abstract = "A simple and rapid method is described for analyzing the Monascus pigment-mediated degradation of 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2(NHOH)). We used the in-capillary micellar electrokinetic chromatography (MEKC). During the electrophoresis, the mutagen and the pigment, due to their different migration velocities, mix for a certain period of time to interact, and then they are separated and quantified. Using this technique, we were able to demonstrate that Trp-P-2(NHOH) is degraded by the pigment. The degradation was pigment-dose dependent, and because the pigment was recovered unchanged, it was deduced that the pigment acted catalytically for the degradation. The entire MEKC procedure takes 8 min. Copyright (C) 1999 Elsevier Science B.V.",
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AU - Mazumder, Tapan Kumar

AU - Yamamoto, Akira

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AU - Arimoto, Sakae

AU - Hayatsu, Hikoya

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