TY - JOUR
T1 - A simple and rapid method for analyzing the Monascus pigment-mediated degradation of mutagenic 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole by in-capillary micellar electrokinetic chromatography
AU - Watanabe, Toshiro
AU - Mazumder, Tapan Kumar
AU - Yamamoto, Akira
AU - Nagai, Shiro
AU - Arimoto-Kobayashi, Sakae
AU - Hayatsu, Hikoya
AU - Terabe, Shigeru
PY - 1999/7/21
Y1 - 1999/7/21
N2 - A simple and rapid method is described for analyzing the Monascus pigment-mediated degradation of 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2(NHOH)). We used the in-capillary micellar electrokinetic chromatography (MEKC). During the electrophoresis, the mutagen and the pigment, due to their different migration velocities, mix for a certain period of time to interact, and then they are separated and quantified. Using this technique, we were able to demonstrate that Trp-P-2(NHOH) is degraded by the pigment. The degradation was pigment-dose dependent, and because the pigment was recovered unchanged, it was deduced that the pigment acted catalytically for the degradation. The entire MEKC procedure takes 8 min. Copyright (C) 1999 Elsevier Science B.V.
AB - A simple and rapid method is described for analyzing the Monascus pigment-mediated degradation of 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2(NHOH)). We used the in-capillary micellar electrokinetic chromatography (MEKC). During the electrophoresis, the mutagen and the pigment, due to their different migration velocities, mix for a certain period of time to interact, and then they are separated and quantified. Using this technique, we were able to demonstrate that Trp-P-2(NHOH) is degraded by the pigment. The degradation was pigment-dose dependent, and because the pigment was recovered unchanged, it was deduced that the pigment acted catalytically for the degradation. The entire MEKC procedure takes 8 min. Copyright (C) 1999 Elsevier Science B.V.
KW - Antimutagenicity
KW - Degradation of mutagen
KW - Heterocyclic amine
KW - Micellar electrokinetic chromatography
KW - Monascus
UR - http://www.scopus.com/inward/record.url?scp=0032799137&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032799137&partnerID=8YFLogxK
U2 - 10.1016/S1383-5718(99)00096-0
DO - 10.1016/S1383-5718(99)00096-0
M3 - Article
C2 - 10477341
AN - SCOPUS:0032799137
SN - 1383-5718
VL - 444
SP - 75
EP - 83
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
IS - 1
ER -