A simple and quick method to isolate nuclear extracts from pupae of Drosophila melanogaster

Haruhisa Kawasaki, Susumu Hirose, Hitoshi Ueda

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Preparation of nuclear extracts is a critical step for biochemical identification of factors which function in the nuclei such as transcription factors. We have established a new method to prepare nuclear extracts from prepupae or pupae of Drosophila melanogaster. The method is simple and particularly useful for small-scale isolation from materials that are hard to get in large amounts such as specific tissues or animal bodies covered by pupal cases.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalCytotechnology
Volume49
Issue number1
DOIs
Publication statusPublished - Sep 2005

Keywords

  • DNA binding factor
  • Drosophila
  • Gel mobility shift assay
  • Metamorphosis
  • Nuclear extract

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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