A simple and quick method to isolate nuclear extracts from pupae of Drosophila melanogaster

Haruhisa Kawasaki; Susumu Hirose; Hitoshi Ueda

doi:10.1007/s10616-005-5414-3

A simple and quick method to isolate nuclear extracts from pupae of Drosophila melanogaster

Haruhisa Kawasaki, Susumu Hirose, Hitoshi Ueda

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Preparation of nuclear extracts is a critical step for biochemical identification of factors which function in the nuclei such as transcription factors. We have established a new method to prepare nuclear extracts from prepupae or pupae of Drosophila melanogaster. The method is simple and particularly useful for small-scale isolation from materials that are hard to get in large amounts such as specific tissues or animal bodies covered by pupal cases.

Original language	English
Pages (from-to)	67-70
Number of pages	4
Journal	Cytotechnology
Volume	49
Issue number	1
DOIs	https://doi.org/10.1007/s10616-005-5414-3
Publication status	Published - Sept 2005

Keywords

DNA binding factor
Drosophila
Gel mobility shift assay
Metamorphosis
Nuclear extract

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Clinical Biochemistry
Cell Biology

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10.1007/s10616-005-5414-3

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AU - Hirose, Susumu

AU - Ueda, Hitoshi

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AB - Preparation of nuclear extracts is a critical step for biochemical identification of factors which function in the nuclei such as transcription factors. We have established a new method to prepare nuclear extracts from prepupae or pupae of Drosophila melanogaster. The method is simple and particularly useful for small-scale isolation from materials that are hard to get in large amounts such as specific tissues or animal bodies covered by pupal cases.

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KW - Gel mobility shift assay

KW - Metamorphosis

KW - Nuclear extract

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A simple and quick method to isolate nuclear extracts from pupae of Drosophila melanogaster

Abstract

Keywords

ASJC Scopus subject areas

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